New insights into the mycorrhizal Rhizoscyphus ericae aggregate: spatial structure and co-colonization of ectomycorrhizal and ericoid roots

Gwen-Aelle Grelet, David Johnson, Trude Vralstad, Ian J. Alexander, Ian C. Anderson

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

P>Fungi in the Rhizoscyphus ericae aggregate have been recovered from the roots of co-occurring ericaceous shrubs and ectomycorrhizal trees. However, to date, there is no evidence that the same individual genotypes colonize both hosts, and no information on the extent of the mycelial networks that might form.

Using spatially explicit core sampling, we isolated fungi from neighbouring Pinus sylvestris (ectomycorrhizal) and Vaccinium vitis-idaea (ericoid mycorrhizal) roots and applied intersimple sequence repeat (ISSR) typing to assess the occurrence and extent of shared genets.

Most isolates were identified as Meliniomyces variabilis, and isolates with identical ISSR profiles were obtained from neighbouring ericoid and ectomycorrhizal roots on a number of occasions. However, genet sizes were small (< 13 cm), and several genets were found in a single soil core. Genetic relatedness was independent of spatial separation at the scales investigated (< 43 m) and M. variabilis populations from sites 20 km apart were genetically indistinguishable.

We conclude that individual genets of M. variabilis can simultaneously colonize Scots pine and Vaccinium roots, but there is no evidence for the formation of large mycelial networks. Our data also suggest significant genotypic overlap between widely separated populations of this ubiquitous root-associated fungus.

Original languageEnglish
Pages (from-to)210-222
Number of pages13
JournalNew Phytologist
Volume188
Issue number1
DOIs
Publication statusPublished - 2010

Keywords

  • ericoid mycorrhizas
  • fungal genets
  • fungal networks
  • Meliniomyces
  • Rhizoscyphus ericae aggregate
  • endophyte phialocephala-fortinii
  • epacris-pulchella ericaceae
  • neighbor-joining method
  • direct DNA extraction
  • meliniomyces-variabilis
  • cenococcum-geophilum
  • autocorrelation analysis
  • microsatellite markers
  • piceirhiza-bicolorata
  • molecular diversity

Cite this

New insights into the mycorrhizal Rhizoscyphus ericae aggregate : spatial structure and co-colonization of ectomycorrhizal and ericoid roots. / Grelet, Gwen-Aelle; Johnson, David; Vralstad, Trude; Alexander, Ian J.; Anderson, Ian C.

In: New Phytologist, Vol. 188, No. 1, 2010, p. 210-222.

Research output: Contribution to journalArticle

Grelet, Gwen-Aelle ; Johnson, David ; Vralstad, Trude ; Alexander, Ian J. ; Anderson, Ian C. / New insights into the mycorrhizal Rhizoscyphus ericae aggregate : spatial structure and co-colonization of ectomycorrhizal and ericoid roots. In: New Phytologist. 2010 ; Vol. 188, No. 1. pp. 210-222.
@article{11d8953911eb4344a1f7ed6938f7d8bb,
title = "New insights into the mycorrhizal Rhizoscyphus ericae aggregate: spatial structure and co-colonization of ectomycorrhizal and ericoid roots",
abstract = "P>Fungi in the Rhizoscyphus ericae aggregate have been recovered from the roots of co-occurring ericaceous shrubs and ectomycorrhizal trees. However, to date, there is no evidence that the same individual genotypes colonize both hosts, and no information on the extent of the mycelial networks that might form.Using spatially explicit core sampling, we isolated fungi from neighbouring Pinus sylvestris (ectomycorrhizal) and Vaccinium vitis-idaea (ericoid mycorrhizal) roots and applied intersimple sequence repeat (ISSR) typing to assess the occurrence and extent of shared genets.Most isolates were identified as Meliniomyces variabilis, and isolates with identical ISSR profiles were obtained from neighbouring ericoid and ectomycorrhizal roots on a number of occasions. However, genet sizes were small (< 13 cm), and several genets were found in a single soil core. Genetic relatedness was independent of spatial separation at the scales investigated (< 43 m) and M. variabilis populations from sites 20 km apart were genetically indistinguishable.We conclude that individual genets of M. variabilis can simultaneously colonize Scots pine and Vaccinium roots, but there is no evidence for the formation of large mycelial networks. Our data also suggest significant genotypic overlap between widely separated populations of this ubiquitous root-associated fungus.",
keywords = "ericoid mycorrhizas, fungal genets, fungal networks, Meliniomyces, Rhizoscyphus ericae aggregate, endophyte phialocephala-fortinii, epacris-pulchella ericaceae, neighbor-joining method, direct DNA extraction, meliniomyces-variabilis, cenococcum-geophilum, autocorrelation analysis, microsatellite markers, piceirhiza-bicolorata, molecular diversity",
author = "Gwen-Aelle Grelet and David Johnson and Trude Vralstad and Alexander, {Ian J.} and Anderson, {Ian C.}",
year = "2010",
doi = "10.1111/j.1469-8137.2010.03353.x",
language = "English",
volume = "188",
pages = "210--222",
journal = "New Phytologist",
issn = "0028-646X",
publisher = "Wiley/Blackwell (10.1111)",
number = "1",

}

TY - JOUR

T1 - New insights into the mycorrhizal Rhizoscyphus ericae aggregate

T2 - spatial structure and co-colonization of ectomycorrhizal and ericoid roots

AU - Grelet, Gwen-Aelle

AU - Johnson, David

AU - Vralstad, Trude

AU - Alexander, Ian J.

AU - Anderson, Ian C.

PY - 2010

Y1 - 2010

N2 - P>Fungi in the Rhizoscyphus ericae aggregate have been recovered from the roots of co-occurring ericaceous shrubs and ectomycorrhizal trees. However, to date, there is no evidence that the same individual genotypes colonize both hosts, and no information on the extent of the mycelial networks that might form.Using spatially explicit core sampling, we isolated fungi from neighbouring Pinus sylvestris (ectomycorrhizal) and Vaccinium vitis-idaea (ericoid mycorrhizal) roots and applied intersimple sequence repeat (ISSR) typing to assess the occurrence and extent of shared genets.Most isolates were identified as Meliniomyces variabilis, and isolates with identical ISSR profiles were obtained from neighbouring ericoid and ectomycorrhizal roots on a number of occasions. However, genet sizes were small (< 13 cm), and several genets were found in a single soil core. Genetic relatedness was independent of spatial separation at the scales investigated (< 43 m) and M. variabilis populations from sites 20 km apart were genetically indistinguishable.We conclude that individual genets of M. variabilis can simultaneously colonize Scots pine and Vaccinium roots, but there is no evidence for the formation of large mycelial networks. Our data also suggest significant genotypic overlap between widely separated populations of this ubiquitous root-associated fungus.

AB - P>Fungi in the Rhizoscyphus ericae aggregate have been recovered from the roots of co-occurring ericaceous shrubs and ectomycorrhizal trees. However, to date, there is no evidence that the same individual genotypes colonize both hosts, and no information on the extent of the mycelial networks that might form.Using spatially explicit core sampling, we isolated fungi from neighbouring Pinus sylvestris (ectomycorrhizal) and Vaccinium vitis-idaea (ericoid mycorrhizal) roots and applied intersimple sequence repeat (ISSR) typing to assess the occurrence and extent of shared genets.Most isolates were identified as Meliniomyces variabilis, and isolates with identical ISSR profiles were obtained from neighbouring ericoid and ectomycorrhizal roots on a number of occasions. However, genet sizes were small (< 13 cm), and several genets were found in a single soil core. Genetic relatedness was independent of spatial separation at the scales investigated (< 43 m) and M. variabilis populations from sites 20 km apart were genetically indistinguishable.We conclude that individual genets of M. variabilis can simultaneously colonize Scots pine and Vaccinium roots, but there is no evidence for the formation of large mycelial networks. Our data also suggest significant genotypic overlap between widely separated populations of this ubiquitous root-associated fungus.

KW - ericoid mycorrhizas

KW - fungal genets

KW - fungal networks

KW - Meliniomyces

KW - Rhizoscyphus ericae aggregate

KW - endophyte phialocephala-fortinii

KW - epacris-pulchella ericaceae

KW - neighbor-joining method

KW - direct DNA extraction

KW - meliniomyces-variabilis

KW - cenococcum-geophilum

KW - autocorrelation analysis

KW - microsatellite markers

KW - piceirhiza-bicolorata

KW - molecular diversity

U2 - 10.1111/j.1469-8137.2010.03353.x

DO - 10.1111/j.1469-8137.2010.03353.x

M3 - Article

VL - 188

SP - 210

EP - 222

JO - New Phytologist

JF - New Phytologist

SN - 0028-646X

IS - 1

ER -