Nitric oxide synthesis in patients with infective gastroenteritis

P Forte, R S Dykhuizen, Eric Milne, A McKenzie, C C Smith, N Benjamin

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    Abstract

    Background-There is evidence that endogenous nitrate synthesis is notably increased in patients with infective gastroenteritis.

    Aims-To determine whether this is due to nitric oxide (NO) production via the L-arginine/NO pathway.

    Methods-Seven male patients with community acquired bacterial gastroenteritis and 15 healthy male volunteers participated in this study, All patients had stool culture positive infective gastroenteritis. A bolus of 200 mg L-[N-15](2)-arginine was administered intravenously after an overnight fast. Urine was collected for the next 36 hours. Urinary [N-15:N-14]nitrate ratio was assessed by dry combustion in an isotope ratio mass spectrometer.

    Results-Mean 36 hour total urinary nitrate excretion in the gastroenteritis group was 5157 (577) mu mol compared with 2594 (234) mu mol in the control group (p<0.001). Thirty six hour urinary [N-15]nitrate excretion was considerably higher in the gastroenteritis group compared with the control group (13782 (1665) versus 1698 (98) eta mol; p<0.001). These values represent 1.129 (0.139)% and 0.138 (0.007)% of [N-15]nitrogen administered (p<0.001), respectively. Corrected 36 hour urinary [N-15]nitrate excretion for urinary creatinine was also significantly higher in the patient compared with the control group (1934 (221) versus 303 (35) eta mol/mmol; p<0.001).

    Conclusion-Results show notably enhanced nitrate synthesis due to increased activity of the L-arginine/NO pathway in patients with infective gastroenteritis.

    Original languageEnglish
    Pages (from-to)355-361
    Number of pages7
    JournalGut
    Volume45
    Issue number3
    DOIs
    Publication statusPublished - Sep 1999

    Keywords

    • endothelium derived relaxing factor
    • L-[N-15](2)-arginine
    • nitrates
    • infection
    • diarrhoea
    • inflammatory bowel-disease
    • colon epithelial-cells
    • plasma nitrate concentration
    • active ulcerative-colitis
    • challenge in-vivo
    • synthase activity
    • L-arginine
    • bacterial translocation
    • Chrons-Disease
    • endotoxin challenge

    Cite this

    Forte, P., Dykhuizen, R. S., Milne, E., McKenzie, A., Smith, C. C., & Benjamin, N. (1999). Nitric oxide synthesis in patients with infective gastroenteritis. Gut, 45(3), 355-361. https://doi.org/10.1136/gut.45.3.355