Nitric oxide synthesis in patients with infective gastroenteritis

P Forte, R S Dykhuizen, Eric Milne, A McKenzie, C C Smith, N Benjamin

    Research output: Contribution to journalArticle

    26 Citations (Scopus)

    Abstract

    Background-There is evidence that endogenous nitrate synthesis is notably increased in patients with infective gastroenteritis.

    Aims-To determine whether this is due to nitric oxide (NO) production via the L-arginine/NO pathway.

    Methods-Seven male patients with community acquired bacterial gastroenteritis and 15 healthy male volunteers participated in this study, All patients had stool culture positive infective gastroenteritis. A bolus of 200 mg L-[N-15](2)-arginine was administered intravenously after an overnight fast. Urine was collected for the next 36 hours. Urinary [N-15:N-14]nitrate ratio was assessed by dry combustion in an isotope ratio mass spectrometer.

    Results-Mean 36 hour total urinary nitrate excretion in the gastroenteritis group was 5157 (577) mu mol compared with 2594 (234) mu mol in the control group (p<0.001). Thirty six hour urinary [N-15]nitrate excretion was considerably higher in the gastroenteritis group compared with the control group (13782 (1665) versus 1698 (98) eta mol; p<0.001). These values represent 1.129 (0.139)% and 0.138 (0.007)% of [N-15]nitrogen administered (p<0.001), respectively. Corrected 36 hour urinary [N-15]nitrate excretion for urinary creatinine was also significantly higher in the patient compared with the control group (1934 (221) versus 303 (35) eta mol/mmol; p<0.001).

    Conclusion-Results show notably enhanced nitrate synthesis due to increased activity of the L-arginine/NO pathway in patients with infective gastroenteritis.

    Original languageEnglish
    Pages (from-to)355-361
    Number of pages7
    JournalGut
    Volume45
    Issue number3
    DOIs
    Publication statusPublished - Sep 1999

    Keywords

    • endothelium derived relaxing factor
    • L-[N-15](2)-arginine
    • nitrates
    • infection
    • diarrhoea
    • inflammatory bowel-disease
    • colon epithelial-cells
    • plasma nitrate concentration
    • active ulcerative-colitis
    • challenge in-vivo
    • synthase activity
    • L-arginine
    • bacterial translocation
    • Chrons-Disease
    • endotoxin challenge

    Cite this

    Forte, P., Dykhuizen, R. S., Milne, E., McKenzie, A., Smith, C. C., & Benjamin, N. (1999). Nitric oxide synthesis in patients with infective gastroenteritis. Gut, 45(3), 355-361. https://doi.org/10.1136/gut.45.3.355

    Nitric oxide synthesis in patients with infective gastroenteritis. / Forte, P ; Dykhuizen, R S ; Milne, Eric; McKenzie, A ; Smith, C C ; Benjamin, N .

    In: Gut, Vol. 45, No. 3, 09.1999, p. 355-361.

    Research output: Contribution to journalArticle

    Forte, P, Dykhuizen, RS, Milne, E, McKenzie, A, Smith, CC & Benjamin, N 1999, 'Nitric oxide synthesis in patients with infective gastroenteritis', Gut, vol. 45, no. 3, pp. 355-361. https://doi.org/10.1136/gut.45.3.355
    Forte P, Dykhuizen RS, Milne E, McKenzie A, Smith CC, Benjamin N. Nitric oxide synthesis in patients with infective gastroenteritis. Gut. 1999 Sep;45(3):355-361. https://doi.org/10.1136/gut.45.3.355
    Forte, P ; Dykhuizen, R S ; Milne, Eric ; McKenzie, A ; Smith, C C ; Benjamin, N . / Nitric oxide synthesis in patients with infective gastroenteritis. In: Gut. 1999 ; Vol. 45, No. 3. pp. 355-361.
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    abstract = "Background-There is evidence that endogenous nitrate synthesis is notably increased in patients with infective gastroenteritis.Aims-To determine whether this is due to nitric oxide (NO) production via the L-arginine/NO pathway.Methods-Seven male patients with community acquired bacterial gastroenteritis and 15 healthy male volunteers participated in this study, All patients had stool culture positive infective gastroenteritis. A bolus of 200 mg L-[N-15](2)-arginine was administered intravenously after an overnight fast. Urine was collected for the next 36 hours. Urinary [N-15:N-14]nitrate ratio was assessed by dry combustion in an isotope ratio mass spectrometer.Results-Mean 36 hour total urinary nitrate excretion in the gastroenteritis group was 5157 (577) mu mol compared with 2594 (234) mu mol in the control group (p<0.001). Thirty six hour urinary [N-15]nitrate excretion was considerably higher in the gastroenteritis group compared with the control group (13782 (1665) versus 1698 (98) eta mol; p<0.001). These values represent 1.129 (0.139){\%} and 0.138 (0.007){\%} of [N-15]nitrogen administered (p<0.001), respectively. Corrected 36 hour urinary [N-15]nitrate excretion for urinary creatinine was also significantly higher in the patient compared with the control group (1934 (221) versus 303 (35) eta mol/mmol; p<0.001).Conclusion-Results show notably enhanced nitrate synthesis due to increased activity of the L-arginine/NO pathway in patients with infective gastroenteritis.",
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    author = "P Forte and Dykhuizen, {R S} and Eric Milne and A McKenzie and Smith, {C C} and N Benjamin",
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    T1 - Nitric oxide synthesis in patients with infective gastroenteritis

    AU - Forte, P

    AU - Dykhuizen, R S

    AU - Milne, Eric

    AU - McKenzie, A

    AU - Smith, C C

    AU - Benjamin, N

    PY - 1999/9

    Y1 - 1999/9

    N2 - Background-There is evidence that endogenous nitrate synthesis is notably increased in patients with infective gastroenteritis.Aims-To determine whether this is due to nitric oxide (NO) production via the L-arginine/NO pathway.Methods-Seven male patients with community acquired bacterial gastroenteritis and 15 healthy male volunteers participated in this study, All patients had stool culture positive infective gastroenteritis. A bolus of 200 mg L-[N-15](2)-arginine was administered intravenously after an overnight fast. Urine was collected for the next 36 hours. Urinary [N-15:N-14]nitrate ratio was assessed by dry combustion in an isotope ratio mass spectrometer.Results-Mean 36 hour total urinary nitrate excretion in the gastroenteritis group was 5157 (577) mu mol compared with 2594 (234) mu mol in the control group (p<0.001). Thirty six hour urinary [N-15]nitrate excretion was considerably higher in the gastroenteritis group compared with the control group (13782 (1665) versus 1698 (98) eta mol; p<0.001). These values represent 1.129 (0.139)% and 0.138 (0.007)% of [N-15]nitrogen administered (p<0.001), respectively. Corrected 36 hour urinary [N-15]nitrate excretion for urinary creatinine was also significantly higher in the patient compared with the control group (1934 (221) versus 303 (35) eta mol/mmol; p<0.001).Conclusion-Results show notably enhanced nitrate synthesis due to increased activity of the L-arginine/NO pathway in patients with infective gastroenteritis.

    AB - Background-There is evidence that endogenous nitrate synthesis is notably increased in patients with infective gastroenteritis.Aims-To determine whether this is due to nitric oxide (NO) production via the L-arginine/NO pathway.Methods-Seven male patients with community acquired bacterial gastroenteritis and 15 healthy male volunteers participated in this study, All patients had stool culture positive infective gastroenteritis. A bolus of 200 mg L-[N-15](2)-arginine was administered intravenously after an overnight fast. Urine was collected for the next 36 hours. Urinary [N-15:N-14]nitrate ratio was assessed by dry combustion in an isotope ratio mass spectrometer.Results-Mean 36 hour total urinary nitrate excretion in the gastroenteritis group was 5157 (577) mu mol compared with 2594 (234) mu mol in the control group (p<0.001). Thirty six hour urinary [N-15]nitrate excretion was considerably higher in the gastroenteritis group compared with the control group (13782 (1665) versus 1698 (98) eta mol; p<0.001). These values represent 1.129 (0.139)% and 0.138 (0.007)% of [N-15]nitrogen administered (p<0.001), respectively. Corrected 36 hour urinary [N-15]nitrate excretion for urinary creatinine was also significantly higher in the patient compared with the control group (1934 (221) versus 303 (35) eta mol/mmol; p<0.001).Conclusion-Results show notably enhanced nitrate synthesis due to increased activity of the L-arginine/NO pathway in patients with infective gastroenteritis.

    KW - endothelium derived relaxing factor

    KW - L-[N-15](2)-arginine

    KW - nitrates

    KW - infection

    KW - diarrhoea

    KW - inflammatory bowel-disease

    KW - colon epithelial-cells

    KW - plasma nitrate concentration

    KW - active ulcerative-colitis

    KW - challenge in-vivo

    KW - synthase activity

    KW - L-arginine

    KW - bacterial translocation

    KW - Chrons-Disease

    KW - endotoxin challenge

    U2 - 10.1136/gut.45.3.355

    DO - 10.1136/gut.45.3.355

    M3 - Article

    VL - 45

    SP - 355

    EP - 361

    JO - Gut

    JF - Gut

    SN - 0017-5749

    IS - 3

    ER -