Nitrous oxide production by the ectomycorrhizal fungi Paxillus involutus and Tylospora fibrillosa

Miranda T. Prendergast-Miller, Elizabeth M. Baggs, David Johnson

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Nitrous oxide (N2O) production by filamentous fungi has been demonstrated in pure culture and has been estimated indirectly in soils. However, it is unknown whether ectomycorrhizal fungi can also produce N2O. We demonstrate for the first time the ability of nitrogen (N)-tolerant ectomycorrhizal fungi (Paxillus involutus and Tylospora fibrillosa), found in forest soils under moderate to high rates of N deposition, to produce N2O from nitrate reduction. The N2O concentrations from the ectomycorrhizal fungal treatments after a 10-day pure culture experiment were 0.0117 +/- 0.00015 (P. involutus) and 0.0114 +/- 0.0003 (T. fibrillosa), and 0.0114 +/- 0.00043 mu mol N2O L-1 from a known fungal denitrifier (Fusarium lichenicola). No N2O was detected in the control treatment. Our results indicate the potential for these two N-tolerant ectomycorrhizal fungi to contribute to N2O production. Given that these species are abundant in many forest soils, the strength and regulation of fungal N2O production should now be verified in situ.

Original languageEnglish
Pages (from-to)31-35
Number of pages5
JournalFEMS Microbiology Letters
Volume316
Issue number1
Early online date14 Jan 2011
DOIs
Publication statusPublished - Mar 2011

Keywords

  • ectomycorrhiza
  • fungi
  • nitrous oxide
  • nitrate reduction
  • Paxillus involutus
  • Tylospora fibrillosa
  • fusarium-oxysporum
  • cylindrocarpon-tomkinense
  • pure culture
  • denitrification
  • nitrateq
  • forest
  • soil
  • growth
  • cytochrome-P-450
  • challenges

Cite this

Nitrous oxide production by the ectomycorrhizal fungi Paxillus involutus and Tylospora fibrillosa. / Prendergast-Miller, Miranda T.; Baggs, Elizabeth M.; Johnson, David.

In: FEMS Microbiology Letters, Vol. 316, No. 1, 03.2011, p. 31-35.

Research output: Contribution to journalArticle

Prendergast-Miller, Miranda T. ; Baggs, Elizabeth M. ; Johnson, David. / Nitrous oxide production by the ectomycorrhizal fungi Paxillus involutus and Tylospora fibrillosa. In: FEMS Microbiology Letters. 2011 ; Vol. 316, No. 1. pp. 31-35.
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abstract = "Nitrous oxide (N2O) production by filamentous fungi has been demonstrated in pure culture and has been estimated indirectly in soils. However, it is unknown whether ectomycorrhizal fungi can also produce N2O. We demonstrate for the first time the ability of nitrogen (N)-tolerant ectomycorrhizal fungi (Paxillus involutus and Tylospora fibrillosa), found in forest soils under moderate to high rates of N deposition, to produce N2O from nitrate reduction. The N2O concentrations from the ectomycorrhizal fungal treatments after a 10-day pure culture experiment were 0.0117 +/- 0.00015 (P. involutus) and 0.0114 +/- 0.0003 (T. fibrillosa), and 0.0114 +/- 0.00043 mu mol N2O L-1 from a known fungal denitrifier (Fusarium lichenicola). No N2O was detected in the control treatment. Our results indicate the potential for these two N-tolerant ectomycorrhizal fungi to contribute to N2O production. Given that these species are abundant in many forest soils, the strength and regulation of fungal N2O production should now be verified in situ.",
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