Oligonucleotide Probes That Detect Quantitatively Significant Groups of Butyrate-Producing Bacteria in Human Feces

Georgina Louise Hold, A. Schwiertz, R. I. Aminov, M. Blaut, Harry James Flint

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16S rRNA-targeted oligonucleotide probes were designed for butyrate-producing bacteria from human feces. Three new cluster-specific probes detected bacteria related to Roseburia intestinalis, Faecalibacterium prausnitzii, and Eubacterium hallii at mean populations of 2.3, 3.8, and 0.6%, respectively, in samples from 10 individuals. Additional species-level probes accounted for no more than 1%, with a mean of 7.7%, of the total human fecal microbiota identified as butyrate producers in this study. Bacteria related to E. hallii and the genera Roseburia and Faecalibacterium are therefore among the most abundant known butyrate-producing bacteria in human feces.

Original languageEnglish
Pages (from-to)4320-4324
Number of pages4
JournalApplied and Environmental Microbiology
Issue number7
Publication statusPublished - Jul 2003


  • 16S ribosomal-RNA
  • chain fatty-acids
  • human fecal samples
  • ulcerative-colitis
  • sequence-analysis
  • resistant starch
  • eubacterium SPP.
  • human gut
  • SP-NOV.
  • hybridization

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