Oligonucleotide Probes That Detect Quantitatively Significant Groups of Butyrate-Producing Bacteria in Human Feces

Georgina Louise Hold; A. Schwiertz; R. I. Aminov; M. Blaut; Harry James Flint

doi:10.1128/AEM.69.7.4320-4324.2003

Oligonucleotide Probes That Detect Quantitatively Significant Groups of Butyrate-Producing Bacteria in Human Feces

Georgina Louise Hold, A. Schwiertz, R. I. Aminov, M. Blaut, Harry James Flint

Research output: Contribution to journal › Article › peer-review

267 Citations (Scopus)

Abstract

16S rRNA-targeted oligonucleotide probes were designed for butyrate-producing bacteria from human feces. Three new cluster-specific probes detected bacteria related to Roseburia intestinalis, Faecalibacterium prausnitzii, and Eubacterium hallii at mean populations of 2.3, 3.8, and 0.6%, respectively, in samples from 10 individuals. Additional species-level probes accounted for no more than 1%, with a mean of 7.7%, of the total human fecal microbiota identified as butyrate producers in this study. Bacteria related to E. hallii and the genera Roseburia and Faecalibacterium are therefore among the most abundant known butyrate-producing bacteria in human feces.

Original language	English
Pages (from-to)	4320-4324
Number of pages	4
Journal	Applied and Environmental Microbiology
Volume	69
Issue number	7
DOIs	https://doi.org/10.1128/AEM.69.7.4320-4324.2003
Publication status	Published - Jul 2003

Keywords

16S ribosomal-RNA
chain fatty-acids
human fecal samples
ulcerative-colitis
sequence-analysis
resistant starch
eubacterium SPP.
human gut
SP-NOV.
hybridization

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title = "Oligonucleotide Probes That Detect Quantitatively Significant Groups of Butyrate-Producing Bacteria in Human Feces",

abstract = "16S rRNA-targeted oligonucleotide probes were designed for butyrate-producing bacteria from human feces. Three new cluster-specific probes detected bacteria related to Roseburia intestinalis, Faecalibacterium prausnitzii, and Eubacterium hallii at mean populations of 2.3, 3.8, and 0.6%, respectively, in samples from 10 individuals. Additional species-level probes accounted for no more than 1%, with a mean of 7.7%, of the total human fecal microbiota identified as butyrate producers in this study. Bacteria related to E. hallii and the genera Roseburia and Faecalibacterium are therefore among the most abundant known butyrate-producing bacteria in human feces.",

keywords = "16S ribosomal-RNA, chain fatty-acids, human fecal samples, ulcerative-colitis, sequence-analysis, resistant starch, eubacterium SPP., human gut, SP-NOV., hybridization",

author = "Hold, {Georgina Louise} and A. Schwiertz and Aminov, {R. I.} and M. Blaut and Flint, {Harry James}",

year = "2003",

month = jul,

doi = "10.1128/AEM.69.7.4320-4324.2003",

language = "English",

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journal = "Applied and Environmental Microbiology",

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publisher = "American Society for Microbiology",

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T1 - Oligonucleotide Probes That Detect Quantitatively Significant Groups of Butyrate-Producing Bacteria in Human Feces

AU - Hold, Georgina Louise

AU - Schwiertz, A.

AU - Aminov, R. I.

AU - Blaut, M.

AU - Flint, Harry James

PY - 2003/7

Y1 - 2003/7

N2 - 16S rRNA-targeted oligonucleotide probes were designed for butyrate-producing bacteria from human feces. Three new cluster-specific probes detected bacteria related to Roseburia intestinalis, Faecalibacterium prausnitzii, and Eubacterium hallii at mean populations of 2.3, 3.8, and 0.6%, respectively, in samples from 10 individuals. Additional species-level probes accounted for no more than 1%, with a mean of 7.7%, of the total human fecal microbiota identified as butyrate producers in this study. Bacteria related to E. hallii and the genera Roseburia and Faecalibacterium are therefore among the most abundant known butyrate-producing bacteria in human feces.

AB - 16S rRNA-targeted oligonucleotide probes were designed for butyrate-producing bacteria from human feces. Three new cluster-specific probes detected bacteria related to Roseburia intestinalis, Faecalibacterium prausnitzii, and Eubacterium hallii at mean populations of 2.3, 3.8, and 0.6%, respectively, in samples from 10 individuals. Additional species-level probes accounted for no more than 1%, with a mean of 7.7%, of the total human fecal microbiota identified as butyrate producers in this study. Bacteria related to E. hallii and the genera Roseburia and Faecalibacterium are therefore among the most abundant known butyrate-producing bacteria in human feces.

KW - 16S ribosomal-RNA

KW - chain fatty-acids

KW - human fecal samples

KW - ulcerative-colitis

KW - sequence-analysis

KW - resistant starch

KW - eubacterium SPP.

KW - human gut

KW - SP-NOV.

KW - hybridization

U2 - 10.1128/AEM.69.7.4320-4324.2003

DO - 10.1128/AEM.69.7.4320-4324.2003

M3 - Article

SN - 0099-2240

VL - 69

SP - 4320

EP - 4324

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 7

ER -

Oligonucleotide Probes That Detect Quantitatively Significant Groups of Butyrate-Producing Bacteria in Human Feces

Abstract

Keywords

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