Online stable isotope analysis of dissolved organic carbon size classes using size exclusion chromatography coupled to an isotope ratio mass spectrometer

Ashish Malik, Andrea Scheibe, P. A. Lokabharathi, Gerd Gleixner*

*Corresponding author for this work

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Stable isotopic content of dissolved organic carbon (δ13C- DOC) provides valuable information on its origin and fate. In an attempt to get additional insights into DOC cycling, we developed a method for δ13C measurement of DOC size classes by coupling high-performance liquid chromatography (HPLC)-size exclusion chromatography (SEC) to online isotope ratio mass spectrometry (IRMS). This represents a significant methodological contribution to DOC research. The interface was evaluated using various organic compounds, thoroughly tested with soil-water from a C3-C4 vegetation change experiment, and also applied to riverine and marine DOC. δ13C analysis of standard compounds resulted in excellent analytical precision (<0.3%). Chromatography resolved soil DOC into 3 fractions: high molecular weight (HMW; 0.4-10 kDa), low molecular weight (LMW; 50-400 Da), and retained (R) fraction. Sample reproducibility for measurement of δ13C-DOC size classes was ±0.25% for HMW fraction, ± 0.54% for LMW fraction, and ±1.3% for R fraction. The greater variance in δ13C values of the latter fractions was due to their lower concentrations. The limit of quantification (SD <0.6%) for each size fraction measured as a peak is 200 ng C (2 mg C/L). δ13C-DOC values obtained in SEC mode correlated significantly with those obtained without column in the μEA mode (p < 0.001, intercept 0.17%), which rules out SEC-associated isotopic effects or DOC loss. In the vegetation change experiment, fractions revealed a clear trend in plant contribution to DOC; those in deeper soils and smaller size fractions had less plant material. It was also demonstrated that the technique can be successfully applied to marine and riverine DOC without further sample pretreatment.

Original languageEnglish
Pages (from-to)10123-10129
Number of pages7
JournalEnvironmental Science and Technology
Volume46
Issue number18
DOIs
Publication statusPublished - 18 Sep 2012

Fingerprint

Size exclusion chromatography
Mass spectrometers
Organic carbon
Isotopes
dissolved organic carbon
chromatography
stable isotope
spectrometer
isotope
Soils
Molecular weight
High performance liquid chromatography
Chromatography
Organic compounds
Mass spectrometry
Experiments
Water
vegetation
analysis
liquid chromatography

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry

Cite this

Online stable isotope analysis of dissolved organic carbon size classes using size exclusion chromatography coupled to an isotope ratio mass spectrometer. / Malik, Ashish; Scheibe, Andrea; Lokabharathi, P. A.; Gleixner, Gerd.

In: Environmental Science and Technology, Vol. 46, No. 18, 18.09.2012, p. 10123-10129.

Research output: Contribution to journalArticle

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abstract = "Stable isotopic content of dissolved organic carbon (δ13C- DOC) provides valuable information on its origin and fate. In an attempt to get additional insights into DOC cycling, we developed a method for δ13C measurement of DOC size classes by coupling high-performance liquid chromatography (HPLC)-size exclusion chromatography (SEC) to online isotope ratio mass spectrometry (IRMS). This represents a significant methodological contribution to DOC research. The interface was evaluated using various organic compounds, thoroughly tested with soil-water from a C3-C4 vegetation change experiment, and also applied to riverine and marine DOC. δ13C analysis of standard compounds resulted in excellent analytical precision (<0.3{\%}). Chromatography resolved soil DOC into 3 fractions: high molecular weight (HMW; 0.4-10 kDa), low molecular weight (LMW; 50-400 Da), and retained (R) fraction. Sample reproducibility for measurement of δ13C-DOC size classes was ±0.25{\%} for HMW fraction, ± 0.54{\%} for LMW fraction, and ±1.3{\%} for R fraction. The greater variance in δ13C values of the latter fractions was due to their lower concentrations. The limit of quantification (SD <0.6{\%}) for each size fraction measured as a peak is 200 ng C (2 mg C/L). δ13C-DOC values obtained in SEC mode correlated significantly with those obtained without column in the μEA mode (p < 0.001, intercept 0.17{\%}), which rules out SEC-associated isotopic effects or DOC loss. In the vegetation change experiment, fractions revealed a clear trend in plant contribution to DOC; those in deeper soils and smaller size fractions had less plant material. It was also demonstrated that the technique can be successfully applied to marine and riverine DOC without further sample pretreatment.",
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note = "ACKNOWLEDGMENTS This project was funded by the Max-Planck-Gesellschaft. We acknowledge Deutsche Forschungsgemeinschaft (DFG) for the fellowship to A.M. in the research training group 1257 “Alteration and element mobility at microbe-mineral interface: that is part of the Jena School for Microbial Communication (JSMC). A.M. was also supported by the International Max Planck Research School for Global Biogeochemical Cycles (IMPRS-gBGC). We thank Steffen Ruehlow for technical assistance, Mamatha S.S. for providing marine water samples, and Andreas Schmalwasser for helpful insight. We also thank the anonymous reviewers for insightful comments that helped improve the manuscript.",
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N2 - Stable isotopic content of dissolved organic carbon (δ13C- DOC) provides valuable information on its origin and fate. In an attempt to get additional insights into DOC cycling, we developed a method for δ13C measurement of DOC size classes by coupling high-performance liquid chromatography (HPLC)-size exclusion chromatography (SEC) to online isotope ratio mass spectrometry (IRMS). This represents a significant methodological contribution to DOC research. The interface was evaluated using various organic compounds, thoroughly tested with soil-water from a C3-C4 vegetation change experiment, and also applied to riverine and marine DOC. δ13C analysis of standard compounds resulted in excellent analytical precision (<0.3%). Chromatography resolved soil DOC into 3 fractions: high molecular weight (HMW; 0.4-10 kDa), low molecular weight (LMW; 50-400 Da), and retained (R) fraction. Sample reproducibility for measurement of δ13C-DOC size classes was ±0.25% for HMW fraction, ± 0.54% for LMW fraction, and ±1.3% for R fraction. The greater variance in δ13C values of the latter fractions was due to their lower concentrations. The limit of quantification (SD <0.6%) for each size fraction measured as a peak is 200 ng C (2 mg C/L). δ13C-DOC values obtained in SEC mode correlated significantly with those obtained without column in the μEA mode (p < 0.001, intercept 0.17%), which rules out SEC-associated isotopic effects or DOC loss. In the vegetation change experiment, fractions revealed a clear trend in plant contribution to DOC; those in deeper soils and smaller size fractions had less plant material. It was also demonstrated that the technique can be successfully applied to marine and riverine DOC without further sample pretreatment.

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