Ontogeny of the expression of leptin and its receptor in the murine fetus and placenta

Nigel Hoggard, Leif Hunter, R.g. Lea, P Trayhurn, Julian Mercer

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Leptin is a 167-amino acid protein that is secreted from adipose cells and expressed in placental tissues. It is important nutritionally in the regulation of energy balance, but also has other functions such as a role in reproduction. To investigate the function of the leptin system in fetal development we examined, primarily by in-situ hybridization and immunohistochemistry, the expression (both mRNA and protein) of leptin and its receptor (including the signalling splice variant) in tissues from 11.5, 13.5, 16.5 and 18.5 d postcoitus murine fetuses and associated placentas. We detected leptin mRNA (at low levels) and protein predominantly in the cytotrophoblasts of the labyrinth part of the placenta, an area of nutrient exchange between the developing fetus and the placenta, and in the trophoblast giant cells situated in the junctional zone at the maternal interface. In addition, leptin was strongly expressed in the fetal cartilage-bone and at a lower level in the hair follicles, heart, and liver of the murine fetus at differing stages of development. The leptin receptor, including the signalling splice variant, was also identified in specific fetal tissues. The physiological importance of expression of both leptin and the leptin receptor (OB-R and OB-Rb) in the placenta remains to be determined. In addition, the high levels of expression of leptin and its receptor in discrete areas of the murine fetus suggest that leptin has a critical role in fetal development.
Original languageEnglish
Pages (from-to)317-26
Number of pages10
JournalBritish Journal of Nutrition
Volume83
Issue number3
DOIs
Publication statusPublished - 1 Mar 2000

Fingerprint

Leptin Receptors
Leptin
Placenta
Fetus
Trophoblasts
Fetal Development
Messenger RNA
Proteins
Hair Follicle
Inner Ear
Giant Cells
Cartilage
In Situ Hybridization
Reproduction
Immunohistochemistry
Mothers
Amino Acids
Bone and Bones
Food
Liver

Keywords

  • Animals
  • Carrier Proteins
  • Embryonic and Fetal Development
  • Female
  • Immunohistochemistry
  • In Situ Hybridization
  • Leptin
  • Mice
  • Placenta
  • Pregnancy
  • RNA, Messenger
  • Receptors, Cell Surface
  • Receptors, Leptin
  • Leptin
  • Fetal tissues

Cite this

Ontogeny of the expression of leptin and its receptor in the murine fetus and placenta. / Hoggard, Nigel; Hunter, Leif; Lea, R.g.; Trayhurn, P; Mercer, Julian.

In: British Journal of Nutrition, Vol. 83, No. 3, 01.03.2000, p. 317-26.

Research output: Contribution to journalArticle

@article{b446b24f603241bab55accbdc3322433,
title = "Ontogeny of the expression of leptin and its receptor in the murine fetus and placenta",
abstract = "Leptin is a 167-amino acid protein that is secreted from adipose cells and expressed in placental tissues. It is important nutritionally in the regulation of energy balance, but also has other functions such as a role in reproduction. To investigate the function of the leptin system in fetal development we examined, primarily by in-situ hybridization and immunohistochemistry, the expression (both mRNA and protein) of leptin and its receptor (including the signalling splice variant) in tissues from 11.5, 13.5, 16.5 and 18.5 d postcoitus murine fetuses and associated placentas. We detected leptin mRNA (at low levels) and protein predominantly in the cytotrophoblasts of the labyrinth part of the placenta, an area of nutrient exchange between the developing fetus and the placenta, and in the trophoblast giant cells situated in the junctional zone at the maternal interface. In addition, leptin was strongly expressed in the fetal cartilage-bone and at a lower level in the hair follicles, heart, and liver of the murine fetus at differing stages of development. The leptin receptor, including the signalling splice variant, was also identified in specific fetal tissues. The physiological importance of expression of both leptin and the leptin receptor (OB-R and OB-Rb) in the placenta remains to be determined. In addition, the high levels of expression of leptin and its receptor in discrete areas of the murine fetus suggest that leptin has a critical role in fetal development.",
keywords = "Animals, Carrier Proteins, Embryonic and Fetal Development, Female, Immunohistochemistry, In Situ Hybridization, Leptin, Mice, Placenta, Pregnancy, RNA, Messenger, Receptors, Cell Surface, Receptors, Leptin, Leptin , Fetal tissues",
author = "Nigel Hoggard and Leif Hunter and R.g. Lea and P Trayhurn and Julian Mercer",
year = "2000",
month = "3",
day = "1",
doi = "10.1017/S0007114500000398",
language = "English",
volume = "83",
pages = "317--26",
journal = "British Journal of Nutrition",
issn = "0007-1145",
publisher = "Cambridge Univ. Press.",
number = "3",

}

TY - JOUR

T1 - Ontogeny of the expression of leptin and its receptor in the murine fetus and placenta

AU - Hoggard, Nigel

AU - Hunter, Leif

AU - Lea, R.g.

AU - Trayhurn, P

AU - Mercer, Julian

PY - 2000/3/1

Y1 - 2000/3/1

N2 - Leptin is a 167-amino acid protein that is secreted from adipose cells and expressed in placental tissues. It is important nutritionally in the regulation of energy balance, but also has other functions such as a role in reproduction. To investigate the function of the leptin system in fetal development we examined, primarily by in-situ hybridization and immunohistochemistry, the expression (both mRNA and protein) of leptin and its receptor (including the signalling splice variant) in tissues from 11.5, 13.5, 16.5 and 18.5 d postcoitus murine fetuses and associated placentas. We detected leptin mRNA (at low levels) and protein predominantly in the cytotrophoblasts of the labyrinth part of the placenta, an area of nutrient exchange between the developing fetus and the placenta, and in the trophoblast giant cells situated in the junctional zone at the maternal interface. In addition, leptin was strongly expressed in the fetal cartilage-bone and at a lower level in the hair follicles, heart, and liver of the murine fetus at differing stages of development. The leptin receptor, including the signalling splice variant, was also identified in specific fetal tissues. The physiological importance of expression of both leptin and the leptin receptor (OB-R and OB-Rb) in the placenta remains to be determined. In addition, the high levels of expression of leptin and its receptor in discrete areas of the murine fetus suggest that leptin has a critical role in fetal development.

AB - Leptin is a 167-amino acid protein that is secreted from adipose cells and expressed in placental tissues. It is important nutritionally in the regulation of energy balance, but also has other functions such as a role in reproduction. To investigate the function of the leptin system in fetal development we examined, primarily by in-situ hybridization and immunohistochemistry, the expression (both mRNA and protein) of leptin and its receptor (including the signalling splice variant) in tissues from 11.5, 13.5, 16.5 and 18.5 d postcoitus murine fetuses and associated placentas. We detected leptin mRNA (at low levels) and protein predominantly in the cytotrophoblasts of the labyrinth part of the placenta, an area of nutrient exchange between the developing fetus and the placenta, and in the trophoblast giant cells situated in the junctional zone at the maternal interface. In addition, leptin was strongly expressed in the fetal cartilage-bone and at a lower level in the hair follicles, heart, and liver of the murine fetus at differing stages of development. The leptin receptor, including the signalling splice variant, was also identified in specific fetal tissues. The physiological importance of expression of both leptin and the leptin receptor (OB-R and OB-Rb) in the placenta remains to be determined. In addition, the high levels of expression of leptin and its receptor in discrete areas of the murine fetus suggest that leptin has a critical role in fetal development.

KW - Animals

KW - Carrier Proteins

KW - Embryonic and Fetal Development

KW - Female

KW - Immunohistochemistry

KW - In Situ Hybridization

KW - Leptin

KW - Mice

KW - Placenta

KW - Pregnancy

KW - RNA, Messenger

KW - Receptors, Cell Surface

KW - Receptors, Leptin

KW - Leptin

KW - Fetal tissues

U2 - 10.1017/S0007114500000398

DO - 10.1017/S0007114500000398

M3 - Article

VL - 83

SP - 317

EP - 326

JO - British Journal of Nutrition

JF - British Journal of Nutrition

SN - 0007-1145

IS - 3

ER -