Abstract
| Original language | English |
|---|---|
| Article number | e53855 |
| Number of pages | 8 |
| Journal | Journal of visualized experiments : JoVE |
| Volume | 110 |
| Early online date | 9 Mar 2016 |
| DOIs | |
| Publication status | Published - 5 Apr 2016 |
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Keywords
- lanceolate endings
- hair follicle
- mechanosensation
- ear
- skin
- mouse
- electrophysiology
Cite this
Optical Monitoring of Living Nerve Terminal Labeling in Hair Follicle Lanceolate Endings of the Ex Vivo Mouse Ear Skin. / Bewick, Guy S.; Banks, Robert W.
In: Journal of visualized experiments : JoVE, Vol. 110, e53855, 05.04.2016.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Optical Monitoring of Living Nerve Terminal Labeling in Hair Follicle Lanceolate Endings of the Ex Vivo Mouse Ear Skin
AU - Bewick, Guy S.
AU - Banks, Robert W.
N1 - The work was in part funded by UK Medical Research Council project grant G0601253 to G.S.B. and R.W.B.
PY - 2016/4/5
Y1 - 2016/4/5
N2 - A novel dissection and recording technique is described for optical monitoring staining and de-staining of lanceolate terminals surrounding hair follicles in the skin of the mouse pinna. The preparation is simple and relatively fast, reliably yielding extensive regions of multiple labeled units of living nerve terminals to study uptake and release of styryl pyridinium dyes extensively used in studies of vesicle recycling. Subdividing the preparations before labeling allows test vs. control comparisons in the same ear from a single individual. Helpful tips are given for improving the quality of the preparation, the labeling and the imaging parameters. This new system is suitable for assaying pharmacologically and mechanically-induced uptake and release of these vital dyes in lanceolate terminals in both wild-type and genetically modified animals. Examples of modulatory influences on labeling intensity are given.
AB - A novel dissection and recording technique is described for optical monitoring staining and de-staining of lanceolate terminals surrounding hair follicles in the skin of the mouse pinna. The preparation is simple and relatively fast, reliably yielding extensive regions of multiple labeled units of living nerve terminals to study uptake and release of styryl pyridinium dyes extensively used in studies of vesicle recycling. Subdividing the preparations before labeling allows test vs. control comparisons in the same ear from a single individual. Helpful tips are given for improving the quality of the preparation, the labeling and the imaging parameters. This new system is suitable for assaying pharmacologically and mechanically-induced uptake and release of these vital dyes in lanceolate terminals in both wild-type and genetically modified animals. Examples of modulatory influences on labeling intensity are given.
KW - lanceolate endings
KW - hair follicle
KW - mechanosensation
KW - ear
KW - skin
KW - mouse
KW - electrophysiology
U2 - 10.3791/53855
DO - 10.3791/53855
M3 - Article
VL - 110
JO - Journal of visualized experiments : JoVE
JF - Journal of visualized experiments : JoVE
SN - 1940-087X
M1 - e53855
ER -