Optical Monitoring of Living Nerve Terminal Labeling in Hair Follicle Lanceolate Endings of the Ex Vivo Mouse Ear Skin

Guy S. Bewick, Robert W. Banks

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Abstract

A novel dissection and recording technique is described for optical monitoring staining and de-staining of lanceolate terminals surrounding hair follicles in the skin of the mouse pinna. The preparation is simple and relatively fast, reliably yielding extensive regions of multiple labeled units of living nerve terminals to study uptake and release of styryl pyridinium dyes extensively used in studies of vesicle recycling. Subdividing the preparations before labeling allows test vs. control comparisons in the same ear from a single individual. Helpful tips are given for improving the quality of the preparation, the labeling and the imaging parameters. This new system is suitable for assaying pharmacologically and mechanically-induced uptake and release of these vital dyes in lanceolate terminals in both wild-type and genetically modified animals. Examples of modulatory influences on labeling intensity are given.
Original languageEnglish
Article numbere53855
Number of pages8
JournalJournal of visualized experiments : JoVE
Volume110
Early online date9 Mar 2016
DOIs
Publication statusPublished - 5 Apr 2016

Fingerprint

Hair Follicle
Labeling
Ear
Skin
Coloring Agents
Staining and Labeling
Genetically Modified Animals
Monitoring
Recycling
Dyes
Dissection
Animals
Imaging techniques

Keywords

  • lanceolate endings
  • hair follicle
  • mechanosensation
  • ear
  • skin
  • mouse
  • electrophysiology

Cite this

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title = "Optical Monitoring of Living Nerve Terminal Labeling in Hair Follicle Lanceolate Endings of the Ex Vivo Mouse Ear Skin",
abstract = "A novel dissection and recording technique is described for optical monitoring staining and de-staining of lanceolate terminals surrounding hair follicles in the skin of the mouse pinna. The preparation is simple and relatively fast, reliably yielding extensive regions of multiple labeled units of living nerve terminals to study uptake and release of styryl pyridinium dyes extensively used in studies of vesicle recycling. Subdividing the preparations before labeling allows test vs. control comparisons in the same ear from a single individual. Helpful tips are given for improving the quality of the preparation, the labeling and the imaging parameters. This new system is suitable for assaying pharmacologically and mechanically-induced uptake and release of these vital dyes in lanceolate terminals in both wild-type and genetically modified animals. Examples of modulatory influences on labeling intensity are given.",
keywords = "lanceolate endings, hair follicle, mechanosensation, ear, skin , mouse , electrophysiology",
author = "Bewick, {Guy S.} and Banks, {Robert W.}",
note = "The work was in part funded by UK Medical Research Council project grant G0601253 to G.S.B. and R.W.B.",
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T1 - Optical Monitoring of Living Nerve Terminal Labeling in Hair Follicle Lanceolate Endings of the Ex Vivo Mouse Ear Skin

AU - Bewick, Guy S.

AU - Banks, Robert W.

N1 - The work was in part funded by UK Medical Research Council project grant G0601253 to G.S.B. and R.W.B.

PY - 2016/4/5

Y1 - 2016/4/5

N2 - A novel dissection and recording technique is described for optical monitoring staining and de-staining of lanceolate terminals surrounding hair follicles in the skin of the mouse pinna. The preparation is simple and relatively fast, reliably yielding extensive regions of multiple labeled units of living nerve terminals to study uptake and release of styryl pyridinium dyes extensively used in studies of vesicle recycling. Subdividing the preparations before labeling allows test vs. control comparisons in the same ear from a single individual. Helpful tips are given for improving the quality of the preparation, the labeling and the imaging parameters. This new system is suitable for assaying pharmacologically and mechanically-induced uptake and release of these vital dyes in lanceolate terminals in both wild-type and genetically modified animals. Examples of modulatory influences on labeling intensity are given.

AB - A novel dissection and recording technique is described for optical monitoring staining and de-staining of lanceolate terminals surrounding hair follicles in the skin of the mouse pinna. The preparation is simple and relatively fast, reliably yielding extensive regions of multiple labeled units of living nerve terminals to study uptake and release of styryl pyridinium dyes extensively used in studies of vesicle recycling. Subdividing the preparations before labeling allows test vs. control comparisons in the same ear from a single individual. Helpful tips are given for improving the quality of the preparation, the labeling and the imaging parameters. This new system is suitable for assaying pharmacologically and mechanically-induced uptake and release of these vital dyes in lanceolate terminals in both wild-type and genetically modified animals. Examples of modulatory influences on labeling intensity are given.

KW - lanceolate endings

KW - hair follicle

KW - mechanosensation

KW - ear

KW - skin

KW - mouse

KW - electrophysiology

U2 - 10.3791/53855

DO - 10.3791/53855

M3 - Article

VL - 110

JO - Journal of visualized experiments : JoVE

JF - Journal of visualized experiments : JoVE

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ER -