Optimal combinations of acute phase proteins for detecting infectious disease in pigs

Peter M. H. Heegaard, Anders Stockmarr, Matilde Piñeiro, Rakel Carpintero, Fermin Lampreave, Fiona Margaret Campbell, P. David Eckersall, Mathilda J.M. Toussaint, Erik Gruys, Nanna Skall Sorensen

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    Abstract

    The acute phase protein (APP) response is an early systemic sign of disease, detected as substantial changes in APP serum concentrations and most disease states involving inflammatory reactions give rise to APP responses. To obtain a detailed picture of the general utility of porcine APPs to detect any disease with an inflammatory component seven porcine APPs were analysed in serum sampled at regular intervals in six different experimental challenge groups of pigs, including three bacterial (Actinobacillus pleuropneumoniae, Streptococcus suis, Mycoplasma hyosynoviae), one parasitic (Toxoplasma gondii) and one viral (porcine respiratory and reproductive syndrome virus) infection and one aseptic inflammation. Immunochemical analyses of seven APPs, four positive (C-reactive protein (CRP), haptoglobin (Hp), pig major acute phase protein (pigMAP) and serum amyloid A (SAA)) and three negative (albumin, transthyretin, and apolipoprotein A1 (apoA1)) were performed in the more than 400 serum samples constituting the serum panel. This was followed by advanced statistical treatment of the data using a multi-step procedure which included defining cut-off values and calculating detection probabilities for single APPs and for APP combinations. Combinations of APPs allowed the detection of disease more sensitively than any individual APP and the best three-protein combinations were CRP, apoA1, pigMAP and CRP, apoA1, Hp, respectively, closely followed by the two-protein combinations CRP, pigMAP and apoA1, pigMAP, respectively. For the practical use of such combinations, methodology is described for establishing individual APP threshold values, above which, for any APP in the combination, ongoing infection/inflammation is indicated.

    Original languageEnglish
    Pages (from-to)50
    JournalVeterinary Research
    Volume42
    Early online date1 Mar 2011
    DOIs
    Publication statusPublished - 17 Mar 2011

    Fingerprint

    acute phase proteins
    Acute-Phase Proteins
    infectious diseases
    Communicable Diseases
    Swine
    swine
    apolipoproteins
    C-reactive protein
    C-Reactive Protein
    Apolipoprotein A-I
    Acute-Phase Reaction
    Haptoglobins
    haptoglobins
    Protein C
    Serum
    Mycoplasma hyosynoviae
    inflammation
    Streptococcus suis
    Actinobacillus pleuropneumoniae
    Porcine respiratory and reproductive syndrome virus

    Cite this

    Heegaard, P. M. H., Stockmarr, A., Piñeiro, M., Carpintero, R., Lampreave, F., Campbell, F. M., ... Skall Sorensen, N. (2011). Optimal combinations of acute phase proteins for detecting infectious disease in pigs. Veterinary Research, 42, 50. https://doi.org/10.1186/1297-9716-42-50

    Optimal combinations of acute phase proteins for detecting infectious disease in pigs. / Heegaard, Peter M. H. ; Stockmarr, Anders ; Piñeiro, Matilde ; Carpintero, Rakel ; Lampreave, Fermin ; Campbell, Fiona Margaret; Eckersall, P. David ; Toussaint, Mathilda J.M. ; Gruys, Erik ; Skall Sorensen, Nanna .

    In: Veterinary Research, Vol. 42, 17.03.2011, p. 50.

    Research output: Contribution to journalArticle

    Heegaard, PMH, Stockmarr, A, Piñeiro, M, Carpintero, R, Lampreave, F, Campbell, FM, Eckersall, PD, Toussaint, MJM, Gruys, E & Skall Sorensen, N 2011, 'Optimal combinations of acute phase proteins for detecting infectious disease in pigs', Veterinary Research, vol. 42, pp. 50. https://doi.org/10.1186/1297-9716-42-50
    Heegaard, Peter M. H. ; Stockmarr, Anders ; Piñeiro, Matilde ; Carpintero, Rakel ; Lampreave, Fermin ; Campbell, Fiona Margaret ; Eckersall, P. David ; Toussaint, Mathilda J.M. ; Gruys, Erik ; Skall Sorensen, Nanna . / Optimal combinations of acute phase proteins for detecting infectious disease in pigs. In: Veterinary Research. 2011 ; Vol. 42. pp. 50.
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    AU - Lampreave, Fermin

    AU - Campbell, Fiona Margaret

    AU - Eckersall, P. David

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