Organization of butyrate synthetic genes in human colonic bacteria

phylogenetic conservation and horizontal gene transfer

Petra Louis (Corresponding Author), Sheila I. McCrae, Cédric Charrier, Harry James Flint

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Butyrate producers constitute an important bacterial group in the human large intestine. Butyryl-CoA is formed from two molecules of acetyl-CoA in a process resembling beta-oxidation in reverse. Three different arrangements of the six genes coding for this pathway have been found in low mol% G+C-content Gram-positive human colonic bacteria using DNA sequencing and degenerate PCR. Gene arrangements were strongly conserved within phylogenetic groups defined by 16S rRNA gene sequence relationships. In the case of one of the genes, encoding beta-hydroxybutyryl-CoA dehydrogenase, however, sequence relationships were strongly suggestive of horizontal gene transfer between lineages. The newly identified gene for butyryl-CoA CoA-transferase, which performs the final step in butyrate formation in most known human colonic bacteria, was not closely linked to these central pathway genes.

Original languageEnglish
Pages (from-to)240-247
Number of pages8
JournalFEMS Microbiology Letters
Volume269
Issue number2
Early online date15 Jan 2007
DOIs
Publication statusPublished - Apr 2007

Keywords

  • butyrate
  • human colonic microbiota
  • phylogeny
  • gene arrangement
  • human large-intestine
  • chain fatty-acids
  • clostridium-acetobutylicum
  • genome sequence
  • butyrivibrio-fibrisolvens
  • acetate utilization
  • human feces
  • fermentation
  • proposal
  • pathway

Cite this

Organization of butyrate synthetic genes in human colonic bacteria : phylogenetic conservation and horizontal gene transfer. / Louis, Petra (Corresponding Author); McCrae, Sheila I.; Charrier, Cédric; Flint, Harry James.

In: FEMS Microbiology Letters, Vol. 269, No. 2, 04.2007, p. 240-247.

Research output: Contribution to journalArticle

@article{e007e2cf58c9406abde8fc13fd6bd87b,
title = "Organization of butyrate synthetic genes in human colonic bacteria: phylogenetic conservation and horizontal gene transfer",
abstract = "Butyrate producers constitute an important bacterial group in the human large intestine. Butyryl-CoA is formed from two molecules of acetyl-CoA in a process resembling beta-oxidation in reverse. Three different arrangements of the six genes coding for this pathway have been found in low mol{\%} G+C-content Gram-positive human colonic bacteria using DNA sequencing and degenerate PCR. Gene arrangements were strongly conserved within phylogenetic groups defined by 16S rRNA gene sequence relationships. In the case of one of the genes, encoding beta-hydroxybutyryl-CoA dehydrogenase, however, sequence relationships were strongly suggestive of horizontal gene transfer between lineages. The newly identified gene for butyryl-CoA CoA-transferase, which performs the final step in butyrate formation in most known human colonic bacteria, was not closely linked to these central pathway genes.",
keywords = "butyrate, human colonic microbiota, phylogeny, gene arrangement, human large-intestine, chain fatty-acids, clostridium-acetobutylicum, genome sequence, butyrivibrio-fibrisolvens, acetate utilization, human feces, fermentation, proposal, pathway",
author = "Petra Louis and McCrae, {Sheila I.} and C{\'e}dric Charrier and Flint, {Harry James}",
year = "2007",
month = "4",
doi = "10.1111/j.1574-6968.2006.00629.x",
language = "English",
volume = "269",
pages = "240--247",
journal = "FEMS Microbiology Letters",
issn = "0378-1097",
publisher = "Oxford University Press",
number = "2",

}

TY - JOUR

T1 - Organization of butyrate synthetic genes in human colonic bacteria

T2 - phylogenetic conservation and horizontal gene transfer

AU - Louis, Petra

AU - McCrae, Sheila I.

AU - Charrier, Cédric

AU - Flint, Harry James

PY - 2007/4

Y1 - 2007/4

N2 - Butyrate producers constitute an important bacterial group in the human large intestine. Butyryl-CoA is formed from two molecules of acetyl-CoA in a process resembling beta-oxidation in reverse. Three different arrangements of the six genes coding for this pathway have been found in low mol% G+C-content Gram-positive human colonic bacteria using DNA sequencing and degenerate PCR. Gene arrangements were strongly conserved within phylogenetic groups defined by 16S rRNA gene sequence relationships. In the case of one of the genes, encoding beta-hydroxybutyryl-CoA dehydrogenase, however, sequence relationships were strongly suggestive of horizontal gene transfer between lineages. The newly identified gene for butyryl-CoA CoA-transferase, which performs the final step in butyrate formation in most known human colonic bacteria, was not closely linked to these central pathway genes.

AB - Butyrate producers constitute an important bacterial group in the human large intestine. Butyryl-CoA is formed from two molecules of acetyl-CoA in a process resembling beta-oxidation in reverse. Three different arrangements of the six genes coding for this pathway have been found in low mol% G+C-content Gram-positive human colonic bacteria using DNA sequencing and degenerate PCR. Gene arrangements were strongly conserved within phylogenetic groups defined by 16S rRNA gene sequence relationships. In the case of one of the genes, encoding beta-hydroxybutyryl-CoA dehydrogenase, however, sequence relationships were strongly suggestive of horizontal gene transfer between lineages. The newly identified gene for butyryl-CoA CoA-transferase, which performs the final step in butyrate formation in most known human colonic bacteria, was not closely linked to these central pathway genes.

KW - butyrate

KW - human colonic microbiota

KW - phylogeny

KW - gene arrangement

KW - human large-intestine

KW - chain fatty-acids

KW - clostridium-acetobutylicum

KW - genome sequence

KW - butyrivibrio-fibrisolvens

KW - acetate utilization

KW - human feces

KW - fermentation

KW - proposal

KW - pathway

U2 - 10.1111/j.1574-6968.2006.00629.x

DO - 10.1111/j.1574-6968.2006.00629.x

M3 - Article

VL - 269

SP - 240

EP - 247

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 0378-1097

IS - 2

ER -