Abstract
Butyrate producers constitute an important bacterial group in the human large intestine. Butyryl-CoA is formed from two molecules of acetyl-CoA in a process resembling beta-oxidation in reverse. Three different arrangements of the six genes coding for this pathway have been found in low mol% G+C-content Gram-positive human colonic bacteria using DNA sequencing and degenerate PCR. Gene arrangements were strongly conserved within phylogenetic groups defined by 16S rRNA gene sequence relationships. In the case of one of the genes, encoding beta-hydroxybutyryl-CoA dehydrogenase, however, sequence relationships were strongly suggestive of horizontal gene transfer between lineages. The newly identified gene for butyryl-CoA CoA-transferase, which performs the final step in butyrate formation in most known human colonic bacteria, was not closely linked to these central pathway genes.
| Original language | English |
|---|---|
| Pages (from-to) | 240-247 |
| Number of pages | 8 |
| Journal | FEMS Microbiology Letters |
| Volume | 269 |
| Issue number | 2 |
| Early online date | 15 Jan 2007 |
| DOIs | |
| Publication status | Published - Apr 2007 |
Keywords
- butyrate
- human colonic microbiota
- phylogeny
- gene arrangement
- human large-intestine
- chain fatty-acids
- clostridium-acetobutylicum
- genome sequence
- butyrivibrio-fibrisolvens
- acetate utilization
- human feces
- fermentation
- proposal
- pathway
Cite this
Organization of butyrate synthetic genes in human colonic bacteria : phylogenetic conservation and horizontal gene transfer. / Louis, Petra (Corresponding Author); McCrae, Sheila I.; Charrier, Cédric; Flint, Harry James.
In: FEMS Microbiology Letters, Vol. 269, No. 2, 04.2007, p. 240-247.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Organization of butyrate synthetic genes in human colonic bacteria
T2 - phylogenetic conservation and horizontal gene transfer
AU - Louis, Petra
AU - McCrae, Sheila I.
AU - Charrier, Cédric
AU - Flint, Harry James
PY - 2007/4
Y1 - 2007/4
N2 - Butyrate producers constitute an important bacterial group in the human large intestine. Butyryl-CoA is formed from two molecules of acetyl-CoA in a process resembling beta-oxidation in reverse. Three different arrangements of the six genes coding for this pathway have been found in low mol% G+C-content Gram-positive human colonic bacteria using DNA sequencing and degenerate PCR. Gene arrangements were strongly conserved within phylogenetic groups defined by 16S rRNA gene sequence relationships. In the case of one of the genes, encoding beta-hydroxybutyryl-CoA dehydrogenase, however, sequence relationships were strongly suggestive of horizontal gene transfer between lineages. The newly identified gene for butyryl-CoA CoA-transferase, which performs the final step in butyrate formation in most known human colonic bacteria, was not closely linked to these central pathway genes.
AB - Butyrate producers constitute an important bacterial group in the human large intestine. Butyryl-CoA is formed from two molecules of acetyl-CoA in a process resembling beta-oxidation in reverse. Three different arrangements of the six genes coding for this pathway have been found in low mol% G+C-content Gram-positive human colonic bacteria using DNA sequencing and degenerate PCR. Gene arrangements were strongly conserved within phylogenetic groups defined by 16S rRNA gene sequence relationships. In the case of one of the genes, encoding beta-hydroxybutyryl-CoA dehydrogenase, however, sequence relationships were strongly suggestive of horizontal gene transfer between lineages. The newly identified gene for butyryl-CoA CoA-transferase, which performs the final step in butyrate formation in most known human colonic bacteria, was not closely linked to these central pathway genes.
KW - butyrate
KW - human colonic microbiota
KW - phylogeny
KW - gene arrangement
KW - human large-intestine
KW - chain fatty-acids
KW - clostridium-acetobutylicum
KW - genome sequence
KW - butyrivibrio-fibrisolvens
KW - acetate utilization
KW - human feces
KW - fermentation
KW - proposal
KW - pathway
U2 - 10.1111/j.1574-6968.2006.00629.x
DO - 10.1111/j.1574-6968.2006.00629.x
M3 - Article
VL - 269
SP - 240
EP - 247
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
SN - 0378-1097
IS - 2
ER -