Phloem transport of arsenic species from flag leaf to grain during grain filling

Anne-Marie Carey, Gareth J. Norton, Claire Deacon, Kirk G. Scheckel, Enzo Lombi, Tracy Punshon, Mary Lou Guerinot, Antonio Lanzirotti, Matt Newville, Yongseong Choi, Adam H. Price, Andrew A. Meharg

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

Strategies to reduce arsenic (As) in rice grain, below concentrations that represent a serious human health concern, require that the mechanisms of As accumulation within grain be established. Therefore, retranslocation of As species from flag leaves into filling rice grain was investigated.

Arsenic species were delivered through cut flag leaves during grain fill. Spatial unloading within grains was investigated using synchrotron X-ray fluorescence (SXRF) microtomography. Additionally, the effect of germanic acid (a silicic acid analog) on grain As accumulation in arsenite-treated panicles was examined.

Dimethylarsinic acid (DMA) and monomethylarsonic acid (MMA) were extremely efficiently retranslocated from flag leaves to rice grain; arsenate was poorly retranslocated, and was rapidly reduced to arsenite within flag leaves; arsenite displayed no retranslocation. Within grains, DMA rapidly dispersed while MMA and inorganic As remained close to the entry point. Germanic acid addition did not affect grain As in arsenite-treated panicles. Three-dimensional SXRF microtomography gave further information on arsenite localization in the ovular vascular trace (OVT) of rice grains.

These results demonstrate that inorganic As is poorly remobilized, while organic species are readily remobilized, from leaves to grain. Stem translocation of inorganic As may not rely solely on silicic acid transporters.

Original languageEnglish
Pages (from-to)87-98
Number of pages12
JournalNew Phytologist
Volume192
Issue number1
Early online date10 Jun 2011
DOIs
Publication statusPublished - Oct 2011

Keywords

  • arsenic
  • grain filling
  • phloem
  • rice
  • translocation
  • rice cultivars
  • paddy rice
  • accumulation
  • speciation
  • plants
  • wheat
  • localization
  • silicon
  • barley

Cite this

Carey, A-M., Norton, G. J., Deacon, C., Scheckel, K. G., Lombi, E., Punshon, T., ... Meharg, A. A. (2011). Phloem transport of arsenic species from flag leaf to grain during grain filling. New Phytologist, 192(1), 87-98. https://doi.org/10.1111/j.1469-8137.2011.03789.x

Phloem transport of arsenic species from flag leaf to grain during grain filling. / Carey, Anne-Marie; Norton, Gareth J.; Deacon, Claire; Scheckel, Kirk G.; Lombi, Enzo; Punshon, Tracy; Guerinot, Mary Lou; Lanzirotti, Antonio; Newville, Matt; Choi, Yongseong; Price, Adam H.; Meharg, Andrew A.

In: New Phytologist, Vol. 192, No. 1, 10.2011, p. 87-98.

Research output: Contribution to journalArticle

Carey, A-M, Norton, GJ, Deacon, C, Scheckel, KG, Lombi, E, Punshon, T, Guerinot, ML, Lanzirotti, A, Newville, M, Choi, Y, Price, AH & Meharg, AA 2011, 'Phloem transport of arsenic species from flag leaf to grain during grain filling' New Phytologist, vol. 192, no. 1, pp. 87-98. https://doi.org/10.1111/j.1469-8137.2011.03789.x
Carey, Anne-Marie ; Norton, Gareth J. ; Deacon, Claire ; Scheckel, Kirk G. ; Lombi, Enzo ; Punshon, Tracy ; Guerinot, Mary Lou ; Lanzirotti, Antonio ; Newville, Matt ; Choi, Yongseong ; Price, Adam H. ; Meharg, Andrew A. / Phloem transport of arsenic species from flag leaf to grain during grain filling. In: New Phytologist. 2011 ; Vol. 192, No. 1. pp. 87-98.
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