Phospho-Tau Protein Expression in the Cell Cycle of SH-SY5Y Neuroblastoma Cells: A Morphological Study

Paola Flores-Rodríguez, Charles R. Harrington, Claude M. Wischik, Vanessa Ibarra-Bracamontes, Natanael Zarco, Araceli Navarrete, Alejandra Martínez-Maldonado, Parménides Guadarrama-Ortíz, Ignacio Villanueva-Fierro, Miguel Angel Ontiveros-Torres, George Perry, Alejandra D. Alonso, Benjamin Florán-Garduño, José Segovia (Corresponding Author), José Luna-Muñoz (Corresponding Author)

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Abstract

It has been reported that the main function of tau protein is to stabilize microtubules and promote the movement of organelles through the axon in neurons. In Alzheimer’s disease, tau protein is the major constituent of the paired helical filament, and it undergoes post-translational modifications including hyperphosphorylation and truncation. Whether other functions of tau protein are involved in Alzheimer’s disease is less clear. We used SH-SY5Y human neuroblastoma cells as an in vitro model to further study the functions of tau protein. We detected phosphorylated tau protein as small dense dots in the cell nucleus, which strongly colocalize with intranuclear speckle structures that were also labelled with an antibody to SC35, a protein involved in nuclear RNA splicing. We have shown further that tau protein, phosphorylated at the sites recognized by pT231, TG-3, and AD2 antibodies, is closely associated with cell division. Different functions may be characteristic of phosphorylation at specific sites. Our findings suggest that the presence of tau protein is involved in separation of sister chromatids in anaphase, and that tau protein also participates in maintaining the integrity of the DNA (pT231, prophase) and chromosomes during cell division (TG-3).
Original languageEnglish
Pages (from-to)631-645
Number of pages15
JournalJournal of Alzheimer's Disease
Volume71
Issue number2
Early online date14 Aug 2019
DOIs
Publication statusPublished - 17 Sep 2019

Keywords

  • Alzheimer’s disease
  • cell cycle
  • phospho-tau protein
  • SC35
  • SH-SY5Y
  • staurosporine

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    Flores-Rodríguez, P., Harrington, C. R., Wischik, C. M., Ibarra-Bracamontes, V., Zarco, N., Navarrete, A., Martínez-Maldonado, A., Guadarrama-Ortíz, P., Villanueva-Fierro, I., Ontiveros-Torres, M. A., Perry, G., Alonso, A. D., Florán-Garduño, B., Segovia, J., & Luna-Muñoz, J. (2019). Phospho-Tau Protein Expression in the Cell Cycle of SH-SY5Y Neuroblastoma Cells: A Morphological Study. Journal of Alzheimer's Disease, 71(2), 631-645. https://doi.org/10.3233/JAD-190155