Phospho-Tau Protein Expression in the Cell Cycle of SH-SY5Y Neuroblastoma Cells

A Morphological Study

Paola Flores-Rodríguez, Charles R. Harrington, Claude M. Wischik, Vanessa Ibarra-Bracamontes, Natanael Zarco, Araceli Navarrete, Alejandra Martínez-Maldonado, Parménides Guadarrama-Ortíz, Ignacio Villanueva-Fierro, Miguel Angel Ontiveros-Torres, George Perry, Alejandra D. Alonso, Benjamin Florán-Garduño, José Segovia (Corresponding Author), José Luna-Muñoz (Corresponding Author)

Research output: Contribution to journalArticle

Abstract

It has been reported that the main function of tau protein is to stabilize microtubules and promote the movement of organelles through the axon in neurons. In Alzheimer’s disease, tau protein is the major constituent of the paired helical filament, and it undergoes post-translational modifications including hyperphosphorylation and truncation. Whether other functions of tau protein are involved in Alzheimer’s disease is less clear. We used SH-SY5Y human neuroblastoma cells as an in vitro model to further study the functions of tau protein. We detected phosphorylated tau protein as small dense dots in the cell nucleus, which strongly colocalize with intranuclear speckle structures that were also labelled with an antibody to SC35, a protein involved in nuclear RNA splicing. We have shown further that tau protein, phosphorylated at the sites recognized by pT231, TG-3, and AD2 antibodies, is closely associated with cell division. Different functions may be characteristic of phosphorylation at specific sites. Our findings suggest that the presence of tau protein is involved in separation of sister chromatids in anaphase, and that tau protein also participates in maintaining the integrity of the DNA (pT231, prophase) and chromosomes during cell division (TG-3).
Original languageEnglish
Pages (from-to)631-645
Number of pages15
JournalJournal of Alzheimer's Disease
Volume71
Issue number2
Early online date14 Aug 2019
DOIs
Publication statusPublished - 17 Sep 2019

Fingerprint

tau Proteins
Neuroblastoma
Cell Cycle
Cell Division
Alzheimer Disease
RNA Splicing
Nuclear RNA
Prophase
Anaphase
Chromatids
Antibodies
Post Translational Protein Processing
Cell Nucleus
Microtubules
Organelles
Axons
Chromosomes
Phosphorylation
Neurons
DNA

Keywords

  • Alzheimer’s disease
  • cell cycle
  • phospho-tau protein
  • SC35
  • SH-SY5Y
  • staurosporine

Cite this

Phospho-Tau Protein Expression in the Cell Cycle of SH-SY5Y Neuroblastoma Cells : A Morphological Study. / Flores-Rodríguez, Paola; Harrington, Charles R.; Wischik, Claude M.; Ibarra-Bracamontes, Vanessa; Zarco, Natanael; Navarrete, Araceli; Martínez-Maldonado, Alejandra; Guadarrama-Ortíz, Parménides; Villanueva-Fierro, Ignacio; Ontiveros-Torres, Miguel Angel; Perry, George; Alonso, Alejandra D.; Florán-Garduño, Benjamin; Segovia, José (Corresponding Author); Luna-Muñoz, José (Corresponding Author).

In: Journal of Alzheimer's Disease, Vol. 71, No. 2, 17.09.2019, p. 631-645.

Research output: Contribution to journalArticle

Flores-Rodríguez, P, Harrington, CR, Wischik, CM, Ibarra-Bracamontes, V, Zarco, N, Navarrete, A, Martínez-Maldonado, A, Guadarrama-Ortíz, P, Villanueva-Fierro, I, Ontiveros-Torres, MA, Perry, G, Alonso, AD, Florán-Garduño, B, Segovia, J & Luna-Muñoz, J 2019, 'Phospho-Tau Protein Expression in the Cell Cycle of SH-SY5Y Neuroblastoma Cells: A Morphological Study', Journal of Alzheimer's Disease, vol. 71, no. 2, pp. 631-645. https://doi.org/10.3233/JAD-190155
Flores-Rodríguez, Paola ; Harrington, Charles R. ; Wischik, Claude M. ; Ibarra-Bracamontes, Vanessa ; Zarco, Natanael ; Navarrete, Araceli ; Martínez-Maldonado, Alejandra ; Guadarrama-Ortíz, Parménides ; Villanueva-Fierro, Ignacio ; Ontiveros-Torres, Miguel Angel ; Perry, George ; Alonso, Alejandra D. ; Florán-Garduño, Benjamin ; Segovia, José ; Luna-Muñoz, José. / Phospho-Tau Protein Expression in the Cell Cycle of SH-SY5Y Neuroblastoma Cells : A Morphological Study. In: Journal of Alzheimer's Disease. 2019 ; Vol. 71, No. 2. pp. 631-645.
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abstract = "It has been reported that the main function of tau protein is to stabilize microtubules and promote the movement of organelles through the axon in neurons. In Alzheimer’s disease, tau protein is the major constituent of the paired helical filament, and it undergoes post-translational modifications including hyperphosphorylation and truncation. Whether other functions of tau protein are involved in Alzheimer’s disease is less clear. We used SH-SY5Y human neuroblastoma cells as an in vitro model to further study the functions of tau protein. We detected phosphorylated tau protein as small dense dots in the cell nucleus, which strongly colocalize with intranuclear speckle structures that were also labelled with an antibody to SC35, a protein involved in nuclear RNA splicing. We have shown further that tau protein, phosphorylated at the sites recognized by pT231, TG-3, and AD2 antibodies, is closely associated with cell division. Different functions may be characteristic of phosphorylation at specific sites. Our findings suggest that the presence of tau protein is involved in separation of sister chromatids in anaphase, and that tau protein also participates in maintaining the integrity of the DNA (pT231, prophase) and chromosomes during cell division (TG-3).",
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author = "Paola Flores-Rodr{\'i}guez and Harrington, {Charles R.} and Wischik, {Claude M.} and Vanessa Ibarra-Bracamontes and Natanael Zarco and Araceli Navarrete and Alejandra Mart{\'i}nez-Maldonado and Parm{\'e}nides Guadarrama-Ort{\'i}z and Ignacio Villanueva-Fierro and Ontiveros-Torres, {Miguel Angel} and George Perry and Alonso, {Alejandra D.} and Benjamin Flor{\'a}n-Gardu{\~n}o and Jos{\'e} Segovia and Jos{\'e} Luna-Mu{\~n}oz",
note = "ACKNOWLEDGMENTS Authors want to express their gratitude to Dr. P. Davies (Albert Einstein College of Medicine, Bronx, NY, USA); Lester I. Binder † (North Western, Chicago, IL, USA) for the generous gift of mAbs (TG-3, Alz-50) and (Tau-1, Tau-5, Tau-7), respectively; Tec. Amparo Viramontes Pintos for the handling of the brain tissue; M. en C. Samadhi Moreno-Campuzano for her technical assistance; M en C.J. Iv{\'a}n G{\'a}lvan for his support in confocal microscopy, and the confocal microscopy unit of Laboratorio Nacional de Servicios Experimentales (LaNSE), CINVESTAV. We also want to express our gratitude to the Mexican families who donated brains of loved ones affected with Alzheimer’s disease, and made our research possible. This work is dedicated to the memory of Professor Dr. Jos{\'e} Ra{\'u}l Mena L{\'o}pez †. This work was financially supported by CONACyT grants, No. 142293 (to R.M.), 266492 (I.V-F), 239516 (J.S) and the Mancera-Res{\'e}ndiz family.",
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AU - Ibarra-Bracamontes, Vanessa

AU - Zarco, Natanael

AU - Navarrete, Araceli

AU - Martínez-Maldonado, Alejandra

AU - Guadarrama-Ortíz, Parménides

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