Phosphorylation regulates polarisation of chitin synthesis in Candida albicans

Megan D Lenardon, Sarah Milne, Hector M Mora-Montes, Florian Kaffarnik, Scott Peck, Alistair J P Brown, Carol A Munro, Neil A R Gow

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Abstract

The ability to undergo polarised cell growth is fundamental to the development of almost all walled organisms. Fungi are characterised by yeasts and moulds and both cellular forms have been studied extensively as tractable models of cell polarity. Chitin is a hallmark component of fungal cell walls. Chitin synthesis is essential for growth, viability and rescue from many conditions that impair cell wall integrity. In the polymorphic human pathogen Candida albicans, Chs3 synthesises the majority of chitin in the cell wall and is localised at the tips of growing buds and hyphae, and at the septum. An analysis of the C. albicans phospho-proteome revealed that Chs3 can be phosphorylated at S139. Mutation of this site showed that both phosphorylation and de-phosphorylation were required for the correct localisation and function of Chs3. The kinase Pkc1 was not required to target Chs3 to sites of polarised growth. This is the first report demonstrating an essential role for Chs phosphorylation in the polarised biosynthesis of fungal cell walls and suggests a new mechanism for the regulation of this class of glycosyl transferase enzymes.

Original languageEnglish
Pages (from-to)2199-2206
Number of pages8
JournalJournal of Cell Science
Volume123
Issue number13
Early online date8 Jun 2010
DOIs
Publication statusPublished - 1 Jul 2010

Fingerprint

Chitin
Candida albicans
Cell Wall
Phosphorylation
S 0139
Fungi
Growth
Fungal Structures
Cell Polarity
Hyphae
Proteome
Transferases
Phosphotransferases
Yeasts
Mutation
Enzymes

Keywords

  • chitin
  • cell wall
  • Candida Albicans
  • Chs3
  • Pkc1
  • phosphorylation
  • polarised growth

Cite this

Phosphorylation regulates polarisation of chitin synthesis in Candida albicans. / Lenardon, Megan D; Milne, Sarah; Mora-Montes, Hector M; Kaffarnik, Florian; Peck, Scott; Brown, Alistair J P; Munro, Carol A; Gow, Neil A R.

In: Journal of Cell Science, Vol. 123, No. 13, 01.07.2010, p. 2199-2206.

Research output: Contribution to journalArticle

Lenardon MD, Milne S, Mora-Montes HM, Kaffarnik F, Peck S, Brown AJP et al. Phosphorylation regulates polarisation of chitin synthesis in Candida albicans. Journal of Cell Science. 2010 Jul 1;123(13):2199-2206. https://doi.org/10.1242/jcs.060210
Lenardon, Megan D ; Milne, Sarah ; Mora-Montes, Hector M ; Kaffarnik, Florian ; Peck, Scott ; Brown, Alistair J P ; Munro, Carol A ; Gow, Neil A R. / Phosphorylation regulates polarisation of chitin synthesis in Candida albicans. In: Journal of Cell Science. 2010 ; Vol. 123, No. 13. pp. 2199-2206.
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