Plant nitrogen-use strategy as a driver of rhizosphere archaeal and bacterial ammonia oxidiser abundance

Cécile E Thion (Corresponding Author), Jessica D Poirel, Thomas Cornulier, Franciska T De Vries, Richard D Bardgett, James I Prosser

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The influence of plants on archaeal (AOA) and bacterial (AOB) ammonia oxidisers is poorly understood. Higher microbial activity in the rhizosphere, including organic nitrogen (N) mineralisation, may stimulate both groups, while ammonia uptake by plants may favour AOA, considered to prefer lower ammonia concentration. We therefore hypothesised (i) higher AOA and AOB abundances in the rhizosphere than bulk soil and (ii) that AOA are favoured over AOB in the rhizosphere of plants with an exploitative strategy and high N demand, especially (iii) during early growth, when plant N uptake is higher. These hypotheses were tested by growing 20 grassland plants, covering a spectrum of resource-use strategies, and determining AOA and AOB amoA gene abundances, rhizosphere and bulk soil characteristics and plant functional traits. Joint Bayesian mixed models indicated no increase in AO in the rhizosphere, but revealed that AOA were more abundant in the rhizosphere of exploitative plants, mostly grasses, and less abundant under conservative plants. In contrast, AOB abundance in the rhizosphere and bulk soil depended on pH, rather than plant traits. These findings provide a mechanistic basis for plant-ammonia oxidiser interactions and for links between plant functional traits and ammonia oxidiser ecology.

Original languageEnglish
Article numberfiw091
Number of pages11
JournalFEMS Microbiology Ecology
Volume92
Issue number7
Early online date28 Apr 2016
DOIs
Publication statusPublished - 1 Jul 2016

Fingerprint

Rhizosphere
Ammonia
Nitrogen
Soil
Bacterial Genes
Poaceae
Ecology
Joints

Keywords

  • ammonia-oxidising archaea
  • ammonia-oxidizing bacteria
  • grasslands
  • plant traits
  • resource-use strategy
  • rhizosphere effect

Cite this

Plant nitrogen-use strategy as a driver of rhizosphere archaeal and bacterial ammonia oxidiser abundance. / Thion, Cécile E (Corresponding Author); Poirel, Jessica D; Cornulier, Thomas; De Vries, Franciska T; Bardgett, Richard D; Prosser, James I.

In: FEMS Microbiology Ecology, Vol. 92, No. 7, fiw091, 01.07.2016.

Research output: Contribution to journalArticle

Thion, Cécile E ; Poirel, Jessica D ; Cornulier, Thomas ; De Vries, Franciska T ; Bardgett, Richard D ; Prosser, James I. / Plant nitrogen-use strategy as a driver of rhizosphere archaeal and bacterial ammonia oxidiser abundance. In: FEMS Microbiology Ecology. 2016 ; Vol. 92, No. 7.
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abstract = "The influence of plants on archaeal (AOA) and bacterial (AOB) ammonia oxidisers is poorly understood. Higher microbial activity in the rhizosphere, including organic nitrogen (N) mineralisation, may stimulate both groups, while ammonia uptake by plants may favour AOA, considered to prefer lower ammonia concentration. We therefore hypothesised (i) higher AOA and AOB abundances in the rhizosphere than bulk soil and (ii) that AOA are favoured over AOB in the rhizosphere of plants with an exploitative strategy and high N demand, especially (iii) during early growth, when plant N uptake is higher. These hypotheses were tested by growing 20 grassland plants, covering a spectrum of resource-use strategies, and determining AOA and AOB amoA gene abundances, rhizosphere and bulk soil characteristics and plant functional traits. Joint Bayesian mixed models indicated no increase in AO in the rhizosphere, but revealed that AOA were more abundant in the rhizosphere of exploitative plants, mostly grasses, and less abundant under conservative plants. In contrast, AOB abundance in the rhizosphere and bulk soil depended on pH, rather than plant traits. These findings provide a mechanistic basis for plant-ammonia oxidiser interactions and for links between plant functional traits and ammonia oxidiser ecology.",
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