Pore mutations of the Escherichia coli MscS channel affect desensitization but not ionic preference

Michelle D. Edwards, Wendy Bartlett, Ian R. Booth

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Mechanosensitive channels rescue bacterial cells from a fate of lysis when they transfer from a high- to low-osmolarity environment. Of three Escherichia coli mechanosensitive proteins studied to date, only MscS-Ec demonstrates a small anionic preference and a desensitized, nonconducting state under sustained pressure. Little is known about the mechanisms generating these distinctive properties. Eliminating the sole positive charge in the MscS-Ec pore region (Arg(88)) did not alter anionic preference. Adding positive charges at either end of the pore did not augment anionic preference, and placing negative charges within the pore did not diminish it. Thus, pore charges do not control this characteristic. However, from this analysis we identified mutations in the hinge region of the MscS-Ec pore helix (at Gly(113)) that profoundly affected ability of the channel to desensitize. Substitution with nonpolar (Ala, Pro) or polar (Asp, Arg, Ser) residues inhibited transition to the desensitized state. Interestingly, Gly(113) replaced with Met did not impede desensitization. Thus, although Gly is not specifically required at position 113, MscS desensitization is strongly influenced by the residue situated here. Mutations at residues further into the pore also regulated desensitization. Transition to this unique mechanosensitive channel state is discussed in terms of existing data.

Original languageEnglish
Pages (from-to)3003-3013
Number of pages11
JournalBiophysical Journal
Volume94
Issue number8
DOIs
Publication statusPublished - 15 Apr 2008

Keywords

  • small mechanosensitive channel
  • patch-clamp
  • membrane
  • pressure
  • helix
  • liposomes
  • mechanism
  • glycine
  • cells

Cite this

Pore mutations of the Escherichia coli MscS channel affect desensitization but not ionic preference. / Edwards, Michelle D.; Bartlett, Wendy; Booth, Ian R.

In: Biophysical Journal, Vol. 94, No. 8, 15.04.2008, p. 3003-3013.

Research output: Contribution to journalArticle

Edwards, Michelle D. ; Bartlett, Wendy ; Booth, Ian R. / Pore mutations of the Escherichia coli MscS channel affect desensitization but not ionic preference. In: Biophysical Journal. 2008 ; Vol. 94, No. 8. pp. 3003-3013.
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AB - Mechanosensitive channels rescue bacterial cells from a fate of lysis when they transfer from a high- to low-osmolarity environment. Of three Escherichia coli mechanosensitive proteins studied to date, only MscS-Ec demonstrates a small anionic preference and a desensitized, nonconducting state under sustained pressure. Little is known about the mechanisms generating these distinctive properties. Eliminating the sole positive charge in the MscS-Ec pore region (Arg(88)) did not alter anionic preference. Adding positive charges at either end of the pore did not augment anionic preference, and placing negative charges within the pore did not diminish it. Thus, pore charges do not control this characteristic. However, from this analysis we identified mutations in the hinge region of the MscS-Ec pore helix (at Gly(113)) that profoundly affected ability of the channel to desensitize. Substitution with nonpolar (Ala, Pro) or polar (Asp, Arg, Ser) residues inhibited transition to the desensitized state. Interestingly, Gly(113) replaced with Met did not impede desensitization. Thus, although Gly is not specifically required at position 113, MscS desensitization is strongly influenced by the residue situated here. Mutations at residues further into the pore also regulated desensitization. Transition to this unique mechanosensitive channel state is discussed in terms of existing data.

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