Posttranslational Modifications of Steroid Receptors: Phosphorylation

Dagmara McGuinness, Iain J McEwan

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The detection of phosphorylation status of proteins has become a critical component of the analysis of activity, localization, and turnover studies of most proteins, particularly for those involved in signaling. The androgen receptor is no exception to this rule with its localization, transcriptional activity, and interactions determined by a series of key phosphorylations on serine residues. Here we have presented a series of techniques for the investigation of the phosphorylation status and intracellular localization of the androgen receptor after hormone and growth factor stimulation of cells in culture (in vitro) and in prostate cancer tissue (in vivo). Modified methods for immunohistochemistry, immunoblotting and immunofluorescence detection with high efficacy for the measurement and monitoring of androgen receptor are presented here alongside examples of their use.

Original languageEnglish
Pages (from-to)105-117
Number of pages13
JournalMethods in Molecular Biology
Volume1443
DOIs
Publication statusPublished - 1 Jun 2016

Keywords

  • androgen receptor
  • phosphorylation
  • immunoprecipitation
  • immunohistochemistry
  • immunofluorescence
  • western blot

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