Potent antioxidant and genoprotective effects of boeravinone G, a rotenoid isolated from Boerhaavia diffusa

Gabriella Aviello, Jasna M Canadanovic-Brunet, Natasa Milic, Raffaele Capasso, Ernesto Fattorusso, Orazio Taglialatela-Scafati, Ines Fasolino, Angelo A Izzo, Francesca Borrelli

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

BACKGROUND AND AIMS: Free radicals are implicated in the aetiology of some gastrointestinal disorders such as gastric ulcer, colorectal cancer and inflammatory bowel disease. In the present study we investigated the antioxidant and genoprotective activity of some rotenoids (i.e. boeravinones) isolated from the roots of Boerhaavia diffusa, a plant used in the Ayurvedic medicine for the treatment of diseases affecting the gastrointestinal tract.

METHODS/PRINCIPAL FINDINGS: Antioxidant activity has been evaluated using both chemical (Electron Spin Resonance spectroscopy, ESR) and Caco-2 cells-based (TBARS and ROS) assays. DNA damage was evaluated by Comet assay, while pERK(1/2) and phospho-NF-kB p65 levels were estimated by western blot. Boeravinones G, D and H significantly reduced the signal intensity of ESR induced by hydroxyl radicals, suggesting a scavenging activity. Among rotenoids tested, boeravinone G exerted the most potent effect. Boeravinone G inhibited both TBARS and ROS formation induced by Fenton's reagent, increased SOD activity and reduced H(2)O(2)-induced DNA damage. Finally, boeravinone G reduced the levels of pERK(1) and phospho-NF-kB p65 (but not of pERK(2)) increased by Fenton's reagent.

CONCLUSIONS: It is concluded that boeravinone G exhibits an extraordinary potent antioxidant activity (significant effect in the nanomolar range). The MAP kinase and NF-kB pathways seem to be involved in the antioxidant effect of boeravinone G. Boeravinone G might be considered as lead compound for the development of drugs potentially useful against those pathologies whose aetiology is related to ROS-mediated injuries.

Original languageEnglish
Article numbere19628
JournalPloS ONE
Volume6
Issue number5
DOIs
Publication statusPublished - 20 May 2011

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Nyctaginaceae
rotenoids
transcription factor NF-kappa B
Antioxidants
electron paramagnetic resonance spectroscopy
antioxidants
thiobarbituric acid-reactive substances
DNA damage
etiology
antioxidant activity
NF-kappa B
Ayurvedic medicine
stomach ulcers
Electron spin resonance spectroscopy
inflammatory bowel disease
assays
hydroxyl radicals
digestive system diseases
colorectal neoplasms
mitogen-activated protein kinase

Keywords

  • Antioxidants
  • Blotting, Western
  • Caco-2 Cells
  • Cell Survival
  • DNA Damage
  • DNA Repair
  • Electron Spin Resonance Spectroscopy
  • Flavonoids
  • Humans
  • Hydroxides
  • Lipid Peroxidation
  • MAP Kinase Signaling System
  • Medicine, Ayurvedic
  • NF-kappa B
  • Nyctaginaceae
  • Phytotherapy
  • Plant Extracts
  • Plant Roots
  • Reactive Oxygen Species
  • Signal Transduction
  • Thiobarbituric Acid Reactive Substances
  • Journal Article
  • Research Support, Non-U.S. Gov't

Cite this

Aviello, G., Canadanovic-Brunet, J. M., Milic, N., Capasso, R., Fattorusso, E., Taglialatela-Scafati, O., ... Borrelli, F. (2011). Potent antioxidant and genoprotective effects of boeravinone G, a rotenoid isolated from Boerhaavia diffusa. PloS ONE, 6(5), [e19628]. https://doi.org/10.1371/journal.pone.0019628

Potent antioxidant and genoprotective effects of boeravinone G, a rotenoid isolated from Boerhaavia diffusa. / Aviello, Gabriella; Canadanovic-Brunet, Jasna M; Milic, Natasa; Capasso, Raffaele; Fattorusso, Ernesto; Taglialatela-Scafati, Orazio; Fasolino, Ines; Izzo, Angelo A; Borrelli, Francesca.

In: PloS ONE, Vol. 6, No. 5, e19628, 20.05.2011.

Research output: Contribution to journalArticle

Aviello, G, Canadanovic-Brunet, JM, Milic, N, Capasso, R, Fattorusso, E, Taglialatela-Scafati, O, Fasolino, I, Izzo, AA & Borrelli, F 2011, 'Potent antioxidant and genoprotective effects of boeravinone G, a rotenoid isolated from Boerhaavia diffusa' PloS ONE, vol. 6, no. 5, e19628. https://doi.org/10.1371/journal.pone.0019628
Aviello G, Canadanovic-Brunet JM, Milic N, Capasso R, Fattorusso E, Taglialatela-Scafati O et al. Potent antioxidant and genoprotective effects of boeravinone G, a rotenoid isolated from Boerhaavia diffusa. PloS ONE. 2011 May 20;6(5). e19628. https://doi.org/10.1371/journal.pone.0019628
Aviello, Gabriella ; Canadanovic-Brunet, Jasna M ; Milic, Natasa ; Capasso, Raffaele ; Fattorusso, Ernesto ; Taglialatela-Scafati, Orazio ; Fasolino, Ines ; Izzo, Angelo A ; Borrelli, Francesca. / Potent antioxidant and genoprotective effects of boeravinone G, a rotenoid isolated from Boerhaavia diffusa. In: PloS ONE. 2011 ; Vol. 6, No. 5.
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T1 - Potent antioxidant and genoprotective effects of boeravinone G, a rotenoid isolated from Boerhaavia diffusa

AU - Aviello, Gabriella

AU - Canadanovic-Brunet, Jasna M

AU - Milic, Natasa

AU - Capasso, Raffaele

AU - Fattorusso, Ernesto

AU - Taglialatela-Scafati, Orazio

AU - Fasolino, Ines

AU - Izzo, Angelo A

AU - Borrelli, Francesca

N1 - Funding: This work was supported by the Enrico ed Enrica Sovena Foundation. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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N2 - BACKGROUND AND AIMS: Free radicals are implicated in the aetiology of some gastrointestinal disorders such as gastric ulcer, colorectal cancer and inflammatory bowel disease. In the present study we investigated the antioxidant and genoprotective activity of some rotenoids (i.e. boeravinones) isolated from the roots of Boerhaavia diffusa, a plant used in the Ayurvedic medicine for the treatment of diseases affecting the gastrointestinal tract.METHODS/PRINCIPAL FINDINGS: Antioxidant activity has been evaluated using both chemical (Electron Spin Resonance spectroscopy, ESR) and Caco-2 cells-based (TBARS and ROS) assays. DNA damage was evaluated by Comet assay, while pERK(1/2) and phospho-NF-kB p65 levels were estimated by western blot. Boeravinones G, D and H significantly reduced the signal intensity of ESR induced by hydroxyl radicals, suggesting a scavenging activity. Among rotenoids tested, boeravinone G exerted the most potent effect. Boeravinone G inhibited both TBARS and ROS formation induced by Fenton's reagent, increased SOD activity and reduced H(2)O(2)-induced DNA damage. Finally, boeravinone G reduced the levels of pERK(1) and phospho-NF-kB p65 (but not of pERK(2)) increased by Fenton's reagent.CONCLUSIONS: It is concluded that boeravinone G exhibits an extraordinary potent antioxidant activity (significant effect in the nanomolar range). The MAP kinase and NF-kB pathways seem to be involved in the antioxidant effect of boeravinone G. Boeravinone G might be considered as lead compound for the development of drugs potentially useful against those pathologies whose aetiology is related to ROS-mediated injuries.

AB - BACKGROUND AND AIMS: Free radicals are implicated in the aetiology of some gastrointestinal disorders such as gastric ulcer, colorectal cancer and inflammatory bowel disease. In the present study we investigated the antioxidant and genoprotective activity of some rotenoids (i.e. boeravinones) isolated from the roots of Boerhaavia diffusa, a plant used in the Ayurvedic medicine for the treatment of diseases affecting the gastrointestinal tract.METHODS/PRINCIPAL FINDINGS: Antioxidant activity has been evaluated using both chemical (Electron Spin Resonance spectroscopy, ESR) and Caco-2 cells-based (TBARS and ROS) assays. DNA damage was evaluated by Comet assay, while pERK(1/2) and phospho-NF-kB p65 levels were estimated by western blot. Boeravinones G, D and H significantly reduced the signal intensity of ESR induced by hydroxyl radicals, suggesting a scavenging activity. Among rotenoids tested, boeravinone G exerted the most potent effect. Boeravinone G inhibited both TBARS and ROS formation induced by Fenton's reagent, increased SOD activity and reduced H(2)O(2)-induced DNA damage. Finally, boeravinone G reduced the levels of pERK(1) and phospho-NF-kB p65 (but not of pERK(2)) increased by Fenton's reagent.CONCLUSIONS: It is concluded that boeravinone G exhibits an extraordinary potent antioxidant activity (significant effect in the nanomolar range). The MAP kinase and NF-kB pathways seem to be involved in the antioxidant effect of boeravinone G. Boeravinone G might be considered as lead compound for the development of drugs potentially useful against those pathologies whose aetiology is related to ROS-mediated injuries.

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KW - Blotting, Western

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KW - Cell Survival

KW - DNA Damage

KW - DNA Repair

KW - Electron Spin Resonance Spectroscopy

KW - Flavonoids

KW - Humans

KW - Hydroxides

KW - Lipid Peroxidation

KW - MAP Kinase Signaling System

KW - Medicine, Ayurvedic

KW - NF-kappa B

KW - Nyctaginaceae

KW - Phytotherapy

KW - Plant Extracts

KW - Plant Roots

KW - Reactive Oxygen Species

KW - Signal Transduction

KW - Thiobarbituric Acid Reactive Substances

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

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JO - PloS ONE

JF - PloS ONE

SN - 1932-6203

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ER -