Preferential distribution of long chain polyunsaturated fatty acids in phospatidyl ethanolamine fraction of Guinea pig alveolar apical membranes

S Mukhopadhyay, R Muimo, Fiona Margaret Campbell, Margaret Jane Gordon, A S Monaghan, Asim K Dutta-Roy

Research output: Contribution to journalArticle

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Abstract

We investigated the fatty acid distribution in guinea pig alveolar apical membranes at different developmental stages. Fatty acid composition of the purified membranes isolated from guinea pig fetuses (at 65 day, term = 68 day), neonates (day 1) and adult males was determined. The levels of arachidonic acid (AA) and docosahexaenoic acid (DHA) were higher in the adult guinea pig alveolar apical membrane phosphatidylethanolamine (PE) fraction (9.3 +/- 2.2 and 2.9 +/- 1.0%, respectively) while in other phospholipids (PL) fractions their levels were low or absent(P < 0.01), Furthermore, levels of AA and DHA in the PE fraction of apical membrane increased significantly from fetal (6.6 +/- 3.0 and 0.8 +/- 0.4%, respectively) to neonatal life (10.3 +/- 1.5 and 3.0 +/- 0.8%, respectively). Increase in the level of DHA (almost four-fold) was much more pronounced than that of AA (P < 0.05), As forguinea pig alveolar membranes, EPA and AA were mostly present in the PE fraction in pulmonary adenocarcinoma derived cells (A549 cells), a parallel model of type II pneumocytes, with the levels of AA around three-fold greater than that of EPA, Binding of radiolabelled fatty acids to A549 cells showed no significant differences between the maximum uptake achieved for different fatty acids (AA,1.7 +/- 0.2, EPA, 2.3 +/- 0.3, LA, 1.7 +/- 0.2, OA, 2.0 +/- 0.2 nmol/mg protein, P > 0.5). Once the fatty acids were taken up by these cells AA was mostly identifiable in the monoacylglycerol (MAG) fraction, whereas EPA was equally distributed between the MAG and PL fractions. Oleic acid was mainly present in the triglyceride (TAG) fraction whereas LA was evenly distributed between the TAG, MAG, and PL fractions. Our data demonstrate a preferential distribution of AA and DHA in PE fractions of alveolar apical membranes during development. (C) 2000 Harcourt Publishers Ltd.

Original languageEnglish
Pages (from-to)341-348
Number of pages8
JournalProstaglandins, Leukotrienes and Essential Fatty Acids
Volume62
Issue number6
DOIs
Publication statusPublished - Jun 2000

Keywords

  • CHORIOCARCINOMA BEWO CELLS
  • ARACHIDONIC-ACID
  • FETAL LUNG
  • G-PROTEINS
  • DOCOSAHEXAENOIC ACID
  • HUMAN-NEUTROPHILS
  • BINDING PROTEINS
  • PLASMA-MEMBRANE
  • II PNEUMOCYTES
  • TRANSPORT
  • choriocarcinoma bewo cells
  • arachidonic-acid
  • fetal lung
  • G-proteins
  • docosahexaenoic acid
  • human neutrophils
  • binding proteins
  • plasma-membrane
  • II pneumocytes
  • transport

Cite this

Preferential distribution of long chain polyunsaturated fatty acids in phospatidyl ethanolamine fraction of Guinea pig alveolar apical membranes. / Mukhopadhyay, S ; Muimo, R ; Campbell, Fiona Margaret; Gordon, Margaret Jane; Monaghan, A S ; Dutta-Roy, Asim K.

In: Prostaglandins, Leukotrienes and Essential Fatty Acids, Vol. 62, No. 6, 06.2000, p. 341-348.

Research output: Contribution to journalArticle

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abstract = "We investigated the fatty acid distribution in guinea pig alveolar apical membranes at different developmental stages. Fatty acid composition of the purified membranes isolated from guinea pig fetuses (at 65 day, term = 68 day), neonates (day 1) and adult males was determined. The levels of arachidonic acid (AA) and docosahexaenoic acid (DHA) were higher in the adult guinea pig alveolar apical membrane phosphatidylethanolamine (PE) fraction (9.3 +/- 2.2 and 2.9 +/- 1.0{\%}, respectively) while in other phospholipids (PL) fractions their levels were low or absent(P < 0.01), Furthermore, levels of AA and DHA in the PE fraction of apical membrane increased significantly from fetal (6.6 +/- 3.0 and 0.8 +/- 0.4{\%}, respectively) to neonatal life (10.3 +/- 1.5 and 3.0 +/- 0.8{\%}, respectively). Increase in the level of DHA (almost four-fold) was much more pronounced than that of AA (P < 0.05), As forguinea pig alveolar membranes, EPA and AA were mostly present in the PE fraction in pulmonary adenocarcinoma derived cells (A549 cells), a parallel model of type II pneumocytes, with the levels of AA around three-fold greater than that of EPA, Binding of radiolabelled fatty acids to A549 cells showed no significant differences between the maximum uptake achieved for different fatty acids (AA,1.7 +/- 0.2, EPA, 2.3 +/- 0.3, LA, 1.7 +/- 0.2, OA, 2.0 +/- 0.2 nmol/mg protein, P > 0.5). Once the fatty acids were taken up by these cells AA was mostly identifiable in the monoacylglycerol (MAG) fraction, whereas EPA was equally distributed between the MAG and PL fractions. Oleic acid was mainly present in the triglyceride (TAG) fraction whereas LA was evenly distributed between the TAG, MAG, and PL fractions. Our data demonstrate a preferential distribution of AA and DHA in PE fractions of alveolar apical membranes during development. (C) 2000 Harcourt Publishers Ltd.",
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AU - Mukhopadhyay, S

AU - Muimo, R

AU - Campbell, Fiona Margaret

AU - Gordon, Margaret Jane

AU - Monaghan, A S

AU - Dutta-Roy, Asim K

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N2 - We investigated the fatty acid distribution in guinea pig alveolar apical membranes at different developmental stages. Fatty acid composition of the purified membranes isolated from guinea pig fetuses (at 65 day, term = 68 day), neonates (day 1) and adult males was determined. The levels of arachidonic acid (AA) and docosahexaenoic acid (DHA) were higher in the adult guinea pig alveolar apical membrane phosphatidylethanolamine (PE) fraction (9.3 +/- 2.2 and 2.9 +/- 1.0%, respectively) while in other phospholipids (PL) fractions their levels were low or absent(P < 0.01), Furthermore, levels of AA and DHA in the PE fraction of apical membrane increased significantly from fetal (6.6 +/- 3.0 and 0.8 +/- 0.4%, respectively) to neonatal life (10.3 +/- 1.5 and 3.0 +/- 0.8%, respectively). Increase in the level of DHA (almost four-fold) was much more pronounced than that of AA (P < 0.05), As forguinea pig alveolar membranes, EPA and AA were mostly present in the PE fraction in pulmonary adenocarcinoma derived cells (A549 cells), a parallel model of type II pneumocytes, with the levels of AA around three-fold greater than that of EPA, Binding of radiolabelled fatty acids to A549 cells showed no significant differences between the maximum uptake achieved for different fatty acids (AA,1.7 +/- 0.2, EPA, 2.3 +/- 0.3, LA, 1.7 +/- 0.2, OA, 2.0 +/- 0.2 nmol/mg protein, P > 0.5). Once the fatty acids were taken up by these cells AA was mostly identifiable in the monoacylglycerol (MAG) fraction, whereas EPA was equally distributed between the MAG and PL fractions. Oleic acid was mainly present in the triglyceride (TAG) fraction whereas LA was evenly distributed between the TAG, MAG, and PL fractions. Our data demonstrate a preferential distribution of AA and DHA in PE fractions of alveolar apical membranes during development. (C) 2000 Harcourt Publishers Ltd.

AB - We investigated the fatty acid distribution in guinea pig alveolar apical membranes at different developmental stages. Fatty acid composition of the purified membranes isolated from guinea pig fetuses (at 65 day, term = 68 day), neonates (day 1) and adult males was determined. The levels of arachidonic acid (AA) and docosahexaenoic acid (DHA) were higher in the adult guinea pig alveolar apical membrane phosphatidylethanolamine (PE) fraction (9.3 +/- 2.2 and 2.9 +/- 1.0%, respectively) while in other phospholipids (PL) fractions their levels were low or absent(P < 0.01), Furthermore, levels of AA and DHA in the PE fraction of apical membrane increased significantly from fetal (6.6 +/- 3.0 and 0.8 +/- 0.4%, respectively) to neonatal life (10.3 +/- 1.5 and 3.0 +/- 0.8%, respectively). Increase in the level of DHA (almost four-fold) was much more pronounced than that of AA (P < 0.05), As forguinea pig alveolar membranes, EPA and AA were mostly present in the PE fraction in pulmonary adenocarcinoma derived cells (A549 cells), a parallel model of type II pneumocytes, with the levels of AA around three-fold greater than that of EPA, Binding of radiolabelled fatty acids to A549 cells showed no significant differences between the maximum uptake achieved for different fatty acids (AA,1.7 +/- 0.2, EPA, 2.3 +/- 0.3, LA, 1.7 +/- 0.2, OA, 2.0 +/- 0.2 nmol/mg protein, P > 0.5). Once the fatty acids were taken up by these cells AA was mostly identifiable in the monoacylglycerol (MAG) fraction, whereas EPA was equally distributed between the MAG and PL fractions. Oleic acid was mainly present in the triglyceride (TAG) fraction whereas LA was evenly distributed between the TAG, MAG, and PL fractions. Our data demonstrate a preferential distribution of AA and DHA in PE fractions of alveolar apical membranes during development. (C) 2000 Harcourt Publishers Ltd.

KW - CHORIOCARCINOMA BEWO CELLS

KW - ARACHIDONIC-ACID

KW - FETAL LUNG

KW - G-PROTEINS

KW - DOCOSAHEXAENOIC ACID

KW - HUMAN-NEUTROPHILS

KW - BINDING PROTEINS

KW - PLASMA-MEMBRANE

KW - II PNEUMOCYTES

KW - TRANSPORT

KW - choriocarcinoma bewo cells

KW - arachidonic-acid

KW - fetal lung

KW - G-proteins

KW - docosahexaenoic acid

KW - human neutrophils

KW - binding proteins

KW - plasma-membrane

KW - II pneumocytes

KW - transport

U2 - 10.1054/plef.2000.0164

DO - 10.1054/plef.2000.0164

M3 - Article

VL - 62

SP - 341

EP - 348

JO - Prostaglandins, Leukotrienes and Essential Fatty Acids

JF - Prostaglandins, Leukotrienes and Essential Fatty Acids

SN - 0952-3278

IS - 6

ER -