Preferential uptake of long chain polyunsaturated fatty acids by isolated human placental membranes

Fiona Margaret Campbell, Margaret Jane Gordon, A K DuttaRoy

    Research output: Contribution to journalArticle

    89 Citations (Scopus)

    Abstract

    Fatty acid uptake by the placenta is thought to be a carrier-mediated process, however the mechanism by which long chain polyunsaturated fatty acids (LCPUFA) are preferentially accumulated from the maternal circulation to the fetal tissues is still unclear. To examine the role of the placenta in this process, binding of four different radiolabelled fatty acids ([C-14]oleate, [C-14]linoleate, [C-14]a-linolenate and [C-14]arachidonate) to human placental membranes was studied. Binding of fatty acid was found to be time- and temperature dependent. At equilibrium, the total binding of oleate was highest (5.1 +/- 0.1 nmoles/mg protein) followed by linoleate (2.8 +/- 0.31 nmoles/mg protein) and arachidonate (2.06 +/- 0.4 nmoles/mg protein) and alpha-linolenate binding was lowest (0.5 + 0.1 nmoles/mg protein). However, oleate had the lowest specific binding (37% of the total binding) whereas arachidonate had the highest specific binding (similar to 86% of the total binding) followed by linoleate and a-linolenate (62%, and 69% of the total binding, respectively). Binding of each [C-14] fatty acid was also assessed in the presence of 20-fold excess of other unlabelled ligands. Binding sites seem to have preference for the binding of [C-14] fatty acids in the following order: arachidonic acid >>> linoleic acid >> a-linolenic acid >>>>> oleic acid, whereas BSP and a-tocopherol did not show any competition with any of the [C-14] fatty acids. These data suggest that the fatty acid binding sites in placental membranes are specific for the fatty acids but that they have heterogeneous affinities. Trans fatty acids (elaidic and linoelaidic acids) also competed very strongly for the [C-14] fatty acid binding. Polyclonal antiserum raised against placental FABP(pm) inhibited binding of these [C-14]fatty acids but with variable degrees of inhibition; EFA/LCPUFA binding was much more than that of oleate. Our data suggest that EFA/LCPUFA bound to albumin are preferentially transported by human placental membranes and that the placental FABP(pm) may be involved in the sequestration of EFA/LCPUFA by the placenta.

    Original languageEnglish
    Pages (from-to)77-83
    Number of pages7
    JournalMolecular & Cellular Biochemistry
    Volume155
    Issue number1
    DOIs
    Publication statusPublished - 9 Feb 1996

    Keywords

    • human placenta
    • fatty acid uptake
    • essential fatty acids
    • plasma membrane fatty acid-binding protein (FABP(pm))
    • long chain polyunsaturated fatty acids
    • fetal development
    • RAT-BRAIN
    • METABOLISM
    • LIPIDS
    • DOCOSAHEXAENOATE
    • TRIGLYCERIDES
    • PREGNANCY
    • TRANSPORT
    • PROTEIN
    • INFANTS
    • MOTHERS
    • rat-brain
    • metabolism
    • lipids
    • docosahexaenoate
    • trigylcerides
    • pregnancy
    • transport
    • protein
    • infants
    • mothers

    Cite this

    Preferential uptake of long chain polyunsaturated fatty acids by isolated human placental membranes. / Campbell, Fiona Margaret; Gordon, Margaret Jane; DuttaRoy, A K .

    In: Molecular & Cellular Biochemistry, Vol. 155, No. 1, 09.02.1996, p. 77-83.

    Research output: Contribution to journalArticle

    @article{de537a756a20417fb2752361981f962d,
    title = "Preferential uptake of long chain polyunsaturated fatty acids by isolated human placental membranes",
    abstract = "Fatty acid uptake by the placenta is thought to be a carrier-mediated process, however the mechanism by which long chain polyunsaturated fatty acids (LCPUFA) are preferentially accumulated from the maternal circulation to the fetal tissues is still unclear. To examine the role of the placenta in this process, binding of four different radiolabelled fatty acids ([C-14]oleate, [C-14]linoleate, [C-14]a-linolenate and [C-14]arachidonate) to human placental membranes was studied. Binding of fatty acid was found to be time- and temperature dependent. At equilibrium, the total binding of oleate was highest (5.1 +/- 0.1 nmoles/mg protein) followed by linoleate (2.8 +/- 0.31 nmoles/mg protein) and arachidonate (2.06 +/- 0.4 nmoles/mg protein) and alpha-linolenate binding was lowest (0.5 + 0.1 nmoles/mg protein). However, oleate had the lowest specific binding (37{\%} of the total binding) whereas arachidonate had the highest specific binding (similar to 86{\%} of the total binding) followed by linoleate and a-linolenate (62{\%}, and 69{\%} of the total binding, respectively). Binding of each [C-14] fatty acid was also assessed in the presence of 20-fold excess of other unlabelled ligands. Binding sites seem to have preference for the binding of [C-14] fatty acids in the following order: arachidonic acid >>> linoleic acid >> a-linolenic acid >>>>> oleic acid, whereas BSP and a-tocopherol did not show any competition with any of the [C-14] fatty acids. These data suggest that the fatty acid binding sites in placental membranes are specific for the fatty acids but that they have heterogeneous affinities. Trans fatty acids (elaidic and linoelaidic acids) also competed very strongly for the [C-14] fatty acid binding. Polyclonal antiserum raised against placental FABP(pm) inhibited binding of these [C-14]fatty acids but with variable degrees of inhibition; EFA/LCPUFA binding was much more than that of oleate. Our data suggest that EFA/LCPUFA bound to albumin are preferentially transported by human placental membranes and that the placental FABP(pm) may be involved in the sequestration of EFA/LCPUFA by the placenta.",
    keywords = "human placenta, fatty acid uptake, essential fatty acids, plasma membrane fatty acid-binding protein (FABP(pm)), long chain polyunsaturated fatty acids, fetal development, RAT-BRAIN, METABOLISM, LIPIDS, DOCOSAHEXAENOATE, TRIGLYCERIDES, PREGNANCY, TRANSPORT, PROTEIN, INFANTS, MOTHERS, rat-brain, metabolism, lipids, docosahexaenoate, trigylcerides, pregnancy, transport, protein, infants, mothers",
    author = "Campbell, {Fiona Margaret} and Gordon, {Margaret Jane} and DuttaRoy, {A K}",
    year = "1996",
    month = "2",
    day = "9",
    doi = "10.1007/BF00714336",
    language = "English",
    volume = "155",
    pages = "77--83",
    journal = "Molecular & Cellular Biochemistry",
    issn = "0300-8177",
    publisher = "Springer Netherlands",
    number = "1",

    }

    TY - JOUR

    T1 - Preferential uptake of long chain polyunsaturated fatty acids by isolated human placental membranes

    AU - Campbell, Fiona Margaret

    AU - Gordon, Margaret Jane

    AU - DuttaRoy, A K

    PY - 1996/2/9

    Y1 - 1996/2/9

    N2 - Fatty acid uptake by the placenta is thought to be a carrier-mediated process, however the mechanism by which long chain polyunsaturated fatty acids (LCPUFA) are preferentially accumulated from the maternal circulation to the fetal tissues is still unclear. To examine the role of the placenta in this process, binding of four different radiolabelled fatty acids ([C-14]oleate, [C-14]linoleate, [C-14]a-linolenate and [C-14]arachidonate) to human placental membranes was studied. Binding of fatty acid was found to be time- and temperature dependent. At equilibrium, the total binding of oleate was highest (5.1 +/- 0.1 nmoles/mg protein) followed by linoleate (2.8 +/- 0.31 nmoles/mg protein) and arachidonate (2.06 +/- 0.4 nmoles/mg protein) and alpha-linolenate binding was lowest (0.5 + 0.1 nmoles/mg protein). However, oleate had the lowest specific binding (37% of the total binding) whereas arachidonate had the highest specific binding (similar to 86% of the total binding) followed by linoleate and a-linolenate (62%, and 69% of the total binding, respectively). Binding of each [C-14] fatty acid was also assessed in the presence of 20-fold excess of other unlabelled ligands. Binding sites seem to have preference for the binding of [C-14] fatty acids in the following order: arachidonic acid >>> linoleic acid >> a-linolenic acid >>>>> oleic acid, whereas BSP and a-tocopherol did not show any competition with any of the [C-14] fatty acids. These data suggest that the fatty acid binding sites in placental membranes are specific for the fatty acids but that they have heterogeneous affinities. Trans fatty acids (elaidic and linoelaidic acids) also competed very strongly for the [C-14] fatty acid binding. Polyclonal antiserum raised against placental FABP(pm) inhibited binding of these [C-14]fatty acids but with variable degrees of inhibition; EFA/LCPUFA binding was much more than that of oleate. Our data suggest that EFA/LCPUFA bound to albumin are preferentially transported by human placental membranes and that the placental FABP(pm) may be involved in the sequestration of EFA/LCPUFA by the placenta.

    AB - Fatty acid uptake by the placenta is thought to be a carrier-mediated process, however the mechanism by which long chain polyunsaturated fatty acids (LCPUFA) are preferentially accumulated from the maternal circulation to the fetal tissues is still unclear. To examine the role of the placenta in this process, binding of four different radiolabelled fatty acids ([C-14]oleate, [C-14]linoleate, [C-14]a-linolenate and [C-14]arachidonate) to human placental membranes was studied. Binding of fatty acid was found to be time- and temperature dependent. At equilibrium, the total binding of oleate was highest (5.1 +/- 0.1 nmoles/mg protein) followed by linoleate (2.8 +/- 0.31 nmoles/mg protein) and arachidonate (2.06 +/- 0.4 nmoles/mg protein) and alpha-linolenate binding was lowest (0.5 + 0.1 nmoles/mg protein). However, oleate had the lowest specific binding (37% of the total binding) whereas arachidonate had the highest specific binding (similar to 86% of the total binding) followed by linoleate and a-linolenate (62%, and 69% of the total binding, respectively). Binding of each [C-14] fatty acid was also assessed in the presence of 20-fold excess of other unlabelled ligands. Binding sites seem to have preference for the binding of [C-14] fatty acids in the following order: arachidonic acid >>> linoleic acid >> a-linolenic acid >>>>> oleic acid, whereas BSP and a-tocopherol did not show any competition with any of the [C-14] fatty acids. These data suggest that the fatty acid binding sites in placental membranes are specific for the fatty acids but that they have heterogeneous affinities. Trans fatty acids (elaidic and linoelaidic acids) also competed very strongly for the [C-14] fatty acid binding. Polyclonal antiserum raised against placental FABP(pm) inhibited binding of these [C-14]fatty acids but with variable degrees of inhibition; EFA/LCPUFA binding was much more than that of oleate. Our data suggest that EFA/LCPUFA bound to albumin are preferentially transported by human placental membranes and that the placental FABP(pm) may be involved in the sequestration of EFA/LCPUFA by the placenta.

    KW - human placenta

    KW - fatty acid uptake

    KW - essential fatty acids

    KW - plasma membrane fatty acid-binding protein (FABP(pm))

    KW - long chain polyunsaturated fatty acids

    KW - fetal development

    KW - RAT-BRAIN

    KW - METABOLISM

    KW - LIPIDS

    KW - DOCOSAHEXAENOATE

    KW - TRIGLYCERIDES

    KW - PREGNANCY

    KW - TRANSPORT

    KW - PROTEIN

    KW - INFANTS

    KW - MOTHERS

    KW - rat-brain

    KW - metabolism

    KW - lipids

    KW - docosahexaenoate

    KW - trigylcerides

    KW - pregnancy

    KW - transport

    KW - protein

    KW - infants

    KW - mothers

    U2 - 10.1007/BF00714336

    DO - 10.1007/BF00714336

    M3 - Article

    VL - 155

    SP - 77

    EP - 83

    JO - Molecular & Cellular Biochemistry

    JF - Molecular & Cellular Biochemistry

    SN - 0300-8177

    IS - 1

    ER -