Preparation of total RNA

Alistair J. P. Brown*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)

8 Citations (Scopus)

Abstract

RNA analysis is central to a detailed understanding of gene expression. The expression of many nuclear genes from a wide range of organisms has been studied at the protein level using reporter genes (lacZ, for example), and changes in expression levels in response to specific stimuli are frequently presumed to be mediated at the transcriptional level. However, there are an increasing number of examples of fungal and vertebrate genes that are post-transcriptionally regulated. It is therefore prudent to investigate expression at the RNA and protein levels. This chapter will discuss the isolation of total RNA from yeast. The reader should refer to ref. (1) for a detailed description of the methods for preparation of ribosomal and small RNAs from yeast.
Original languageEnglish
Title of host publicationYeast Protocols
EditorsI H Evans
Place of PublicationClifton, N.J.
PublisherSpringer
Pages269-276
Number of pages8
ISBN (Electronic)978-1-59259-540-2
ISBN (Print)978-0-89603-319-1
DOIs
Publication statusPublished - 25 Oct 1996

Publication series

NameMethods in molecular biology
PublisherHumana Press
Volume53
ISSN (Print)1064-3745

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  • Cite this

    Brown, A. J. P. (1996). Preparation of total RNA. In I. H. Evans (Ed.), Yeast Protocols (pp. 269-276). (Methods in molecular biology; Vol. 53). Springer . https://doi.org/10.1385/0-89603-319-8:269