Pretreatment of Miscanthus× giganteus using Aqueous Ammonia with Hydrogen Peroxide to Increase Enzymatic Hydrolysis to Sugars

Guangren Yu, Waheed Afzal, Fuxin Yang, Sasisanker Padmanabhan, Zhongguo Liu, Hongxue Xie, Mahmoud Abdel Shafy, Alexis T Bell, John M Prausnitz

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

BACKGROUND
Miscanthus×giganteus (M.×giganteus) is a potential source for bioethanol or other useful products. Pretreatment of lignocellulosic biomass is an essential step prior to enzymatic hydrolysis to sugars and fermentation to bioethanol.

RESULTS
In this work, a one-step process uses aqueous ammonia with or without hydrogen peroxide; a proposed two-step process uses aqueous ammonia in the first step and hydrogen peroxide in the second step. In the two-step process, overall 89.5% lignin is removed. The pretreated biomass is followed by using cellulase and β-glucosidase to convert cellulose and hemicellulose from the recovered solid to fermentable sugars. The conversion of cellulose to glucose is 90.2% and to xylose is 73.4%. Characterization data are obtained for the recovered solid using scanning electron microscopy (SEM), attenuated total reflection-infrared spectroscopy (ATR-IR), and X-ray diffraction (XRD) for better understanding of the two-step process.

CONCLUSION
Results from the two-step process using aqueous ammonia and hydrogen peroxide separately are much better than those from the one-step process for removing lignin and for enhancing conversion to sugars by enzymatic hydrolysis. © 2013 Society of Chemical Industry
Original languageEnglish
Pages (from-to)698-706
Number of pages9
JournalJournal of Chemical Technology & Biotechnology
Volume89
Issue number5
DOIs
Publication statusPublished - May 2014

Fingerprint

Enzymatic hydrolysis
Ammonia
Hydrogen peroxide
Sugars
hydrogen peroxide
Hydrogen Peroxide
hydrolysis
sugar
Bioethanol
Hydrolysis
ammonia
Lignin
Cellulose
Biomass
lignin
cellulose
Glucosidases
Xylose
Cellulase
biomass

Keywords

  • Miscanthus×giganteus
  • pretreatment
  • aqueous ammonia
  • hydrogen peroxide
  • enzymatic hydrolysis

Cite this

Pretreatment of Miscanthus× giganteus using Aqueous Ammonia with Hydrogen Peroxide to Increase Enzymatic Hydrolysis to Sugars. / Yu, Guangren; Afzal, Waheed; Yang, Fuxin; Padmanabhan, Sasisanker; Liu, Zhongguo; Xie, Hongxue; Shafy, Mahmoud Abdel; Bell, Alexis T; Prausnitz, John M.

In: Journal of Chemical Technology & Biotechnology, Vol. 89, No. 5, 05.2014, p. 698-706.

Research output: Contribution to journalArticle

Yu, Guangren ; Afzal, Waheed ; Yang, Fuxin ; Padmanabhan, Sasisanker ; Liu, Zhongguo ; Xie, Hongxue ; Shafy, Mahmoud Abdel ; Bell, Alexis T ; Prausnitz, John M. / Pretreatment of Miscanthus× giganteus using Aqueous Ammonia with Hydrogen Peroxide to Increase Enzymatic Hydrolysis to Sugars. In: Journal of Chemical Technology & Biotechnology. 2014 ; Vol. 89, No. 5. pp. 698-706.
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abstract = "BACKGROUNDMiscanthus×giganteus (M.×giganteus) is a potential source for bioethanol or other useful products. Pretreatment of lignocellulosic biomass is an essential step prior to enzymatic hydrolysis to sugars and fermentation to bioethanol.RESULTSIn this work, a one-step process uses aqueous ammonia with or without hydrogen peroxide; a proposed two-step process uses aqueous ammonia in the first step and hydrogen peroxide in the second step. In the two-step process, overall 89.5{\%} lignin is removed. The pretreated biomass is followed by using cellulase and β-glucosidase to convert cellulose and hemicellulose from the recovered solid to fermentable sugars. The conversion of cellulose to glucose is 90.2{\%} and to xylose is 73.4{\%}. Characterization data are obtained for the recovered solid using scanning electron microscopy (SEM), attenuated total reflection-infrared spectroscopy (ATR-IR), and X-ray diffraction (XRD) for better understanding of the two-step process.CONCLUSIONResults from the two-step process using aqueous ammonia and hydrogen peroxide separately are much better than those from the one-step process for removing lignin and for enhancing conversion to sugars by enzymatic hydrolysis. {\circledC} 2013 Society of Chemical Industry",
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author = "Guangren Yu and Waheed Afzal and Fuxin Yang and Sasisanker Padmanabhan and Zhongguo Liu and Hongxue Xie and Shafy, {Mahmoud Abdel} and Bell, {Alexis T} and Prausnitz, {John M}",
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AU - Afzal, Waheed

AU - Yang, Fuxin

AU - Padmanabhan, Sasisanker

AU - Liu, Zhongguo

AU - Xie, Hongxue

AU - Shafy, Mahmoud Abdel

AU - Bell, Alexis T

AU - Prausnitz, John M

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N2 - BACKGROUNDMiscanthus×giganteus (M.×giganteus) is a potential source for bioethanol or other useful products. Pretreatment of lignocellulosic biomass is an essential step prior to enzymatic hydrolysis to sugars and fermentation to bioethanol.RESULTSIn this work, a one-step process uses aqueous ammonia with or without hydrogen peroxide; a proposed two-step process uses aqueous ammonia in the first step and hydrogen peroxide in the second step. In the two-step process, overall 89.5% lignin is removed. The pretreated biomass is followed by using cellulase and β-glucosidase to convert cellulose and hemicellulose from the recovered solid to fermentable sugars. The conversion of cellulose to glucose is 90.2% and to xylose is 73.4%. Characterization data are obtained for the recovered solid using scanning electron microscopy (SEM), attenuated total reflection-infrared spectroscopy (ATR-IR), and X-ray diffraction (XRD) for better understanding of the two-step process.CONCLUSIONResults from the two-step process using aqueous ammonia and hydrogen peroxide separately are much better than those from the one-step process for removing lignin and for enhancing conversion to sugars by enzymatic hydrolysis. © 2013 Society of Chemical Industry

AB - BACKGROUNDMiscanthus×giganteus (M.×giganteus) is a potential source for bioethanol or other useful products. Pretreatment of lignocellulosic biomass is an essential step prior to enzymatic hydrolysis to sugars and fermentation to bioethanol.RESULTSIn this work, a one-step process uses aqueous ammonia with or without hydrogen peroxide; a proposed two-step process uses aqueous ammonia in the first step and hydrogen peroxide in the second step. In the two-step process, overall 89.5% lignin is removed. The pretreated biomass is followed by using cellulase and β-glucosidase to convert cellulose and hemicellulose from the recovered solid to fermentable sugars. The conversion of cellulose to glucose is 90.2% and to xylose is 73.4%. Characterization data are obtained for the recovered solid using scanning electron microscopy (SEM), attenuated total reflection-infrared spectroscopy (ATR-IR), and X-ray diffraction (XRD) for better understanding of the two-step process.CONCLUSIONResults from the two-step process using aqueous ammonia and hydrogen peroxide separately are much better than those from the one-step process for removing lignin and for enhancing conversion to sugars by enzymatic hydrolysis. © 2013 Society of Chemical Industry

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