Production of NO, N2O and N2 by extracted soil bacteria, regulation by NO2- and O2 concentrations

Nicholas Morley, Elizabeth M. Baggs, Peter Dorsch, Lars Bakken

Research output: Contribution to journalArticle

108 Citations (Scopus)

Abstract

The oxygen control of denitrification and its emission of NO/N2O/N-2 was investigated by incubation of Nycodenz-extracted soil bacteria in an incubation robot which monitors O-2, NO, N2O and N-2 concentrations (in He+O-2 atmosphere). Two consecutive incubations were undertaken to determine (1) the regulation of denitrification by O-2 and NO2- during respiratory O-2 depletion and (2) the effects of re-exposure to O-2 of cultures with fully expressed denitrification proteome. Early denitrification was only detected (as NO and N2O) at <= 80 mu M O-2 in treatments with NO2-, and the rates were three orders of magnitude lower than the rates observed after oxygen depletion (with N-2 as the primary product). When re-exposed to O-2, the cultures continued to denitrify (8-55% of the rates during the foregoing anoxic phase), but its main product was N2O. The N2O reductase activity recovered as oxygen was being depleted. The results suggest that expression of the denitrifying proteome may result in significant subsequent aerobic denitrification, and this has profound implications for the understanding and modelling of denitrification and N2O emission. Short anoxic spells caused by transient flooding during rainfall, could lead to subsequent unbalanced aerobic denitrification, in which N2O is a major end product.

Original languageEnglish
Pages (from-to)102-112
Number of pages11
JournalFEMS Microbiology Ecology
Volume65
Issue number1
Early online date28 Jun 2008
DOIs
Publication statusPublished - Jul 2008

Keywords

  • aerobic denitrification
  • oxygen
  • nitrite
  • nitrous oxide
  • nitric oxide
  • dinitrogen
  • ammonia-oxidizing bacteria
  • nitrous-oxide production
  • thiosphaera-pantotropha
  • paracoccus-denitrificans
  • denitrifying bacteria
  • pseudomonas-stutzeri
  • anaerobic conditions
  • continuous-culture

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