Project rheingold: Rapid biosensor analysis of soil and groundwater toxicity

LA Glover*, H Weitz, HL Kuan, S Sousa, K Killham, E Bar, R Henkler

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A toxicity survey can provide invaluable information prior to managed bioremediation of industrially contaminated sites. Furthermore, toxicity monitoring is often required during site remediation to monitor progress. Conventional toxicity assessment involves analytical techniques such as spectrometry, chromatography (HPLC and GC), CC-mass spectrometry and atomic absorption techniques for the determination of heavy metals. These techniques are powerful and sensitive to ppm and ppb levels. However, the ability to detect a compound does not provide information regarding the biological effects of that compound, its persistence in the environment, or information that can be employed in the design of a remediation strategy, lux-marked bacterial biosensors have been found to be rapid and sensitive indicators of toxicity of a wide range of organic and inorganic pollutants. This paper reports the use of lux-marked Escherichia coli (selected as a general indicator) and lux-marked pseudomonads (selected for site relevance) to assess the toxicity of soils and groundwater at a BTEX contaminated site. This approach, coupled with a regime of sample manipulation, allows the determination of the nature of the toxicity as well as providing information on which to base a remediation strategy.

Original languageEnglish
Title of host publicationIn Situ Bioremediation of Petroleum Hydrocarbon and Other Organic Compounds
EditorsBC Alleman, A Leeson
Place of PublicationColumbus
PublisherBattelle Press
Pages369-374
Number of pages6
ISBN (Print)1-57477-076-4
Publication statusPublished - 1999
Event5th International In Situ and On-Site Bioremediation Symposium - SAN DIEGO, Canada
Duration: 19 Apr 199922 Apr 1999

Conference

Conference5th International In Situ and On-Site Bioremediation Symposium
CountryCanada
CitySAN DIEGO
Period19/04/9922/04/99

Keywords

  • PSEUDOMONAS-FLUORESCENS
  • LUMINESCENCE

Cite this

Glover, LA., Weitz, H., Kuan, HL., Sousa, S., Killham, K., Bar, E., & Henkler, R. (1999). Project rheingold: Rapid biosensor analysis of soil and groundwater toxicity. In BC. Alleman, & A. Leeson (Eds.), In Situ Bioremediation of Petroleum Hydrocarbon and Other Organic Compounds (pp. 369-374). Columbus: Battelle Press.

Project rheingold: Rapid biosensor analysis of soil and groundwater toxicity. / Glover, LA; Weitz, H; Kuan, HL; Sousa, S; Killham, K; Bar, E; Henkler, R.

In Situ Bioremediation of Petroleum Hydrocarbon and Other Organic Compounds. ed. / BC Alleman; A Leeson. Columbus : Battelle Press, 1999. p. 369-374.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Glover, LA, Weitz, H, Kuan, HL, Sousa, S, Killham, K, Bar, E & Henkler, R 1999, Project rheingold: Rapid biosensor analysis of soil and groundwater toxicity. in BC Alleman & A Leeson (eds), In Situ Bioremediation of Petroleum Hydrocarbon and Other Organic Compounds. Battelle Press, Columbus, pp. 369-374, 5th International In Situ and On-Site Bioremediation Symposium, SAN DIEGO, Canada, 19/04/99.
Glover LA, Weitz H, Kuan HL, Sousa S, Killham K, Bar E et al. Project rheingold: Rapid biosensor analysis of soil and groundwater toxicity. In Alleman BC, Leeson A, editors, In Situ Bioremediation of Petroleum Hydrocarbon and Other Organic Compounds. Columbus: Battelle Press. 1999. p. 369-374
Glover, LA ; Weitz, H ; Kuan, HL ; Sousa, S ; Killham, K ; Bar, E ; Henkler, R. / Project rheingold: Rapid biosensor analysis of soil and groundwater toxicity. In Situ Bioremediation of Petroleum Hydrocarbon and Other Organic Compounds. editor / BC Alleman ; A Leeson. Columbus : Battelle Press, 1999. pp. 369-374
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AU - Sousa, S

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AU - Bar, E

AU - Henkler, R

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N2 - A toxicity survey can provide invaluable information prior to managed bioremediation of industrially contaminated sites. Furthermore, toxicity monitoring is often required during site remediation to monitor progress. Conventional toxicity assessment involves analytical techniques such as spectrometry, chromatography (HPLC and GC), CC-mass spectrometry and atomic absorption techniques for the determination of heavy metals. These techniques are powerful and sensitive to ppm and ppb levels. However, the ability to detect a compound does not provide information regarding the biological effects of that compound, its persistence in the environment, or information that can be employed in the design of a remediation strategy, lux-marked bacterial biosensors have been found to be rapid and sensitive indicators of toxicity of a wide range of organic and inorganic pollutants. This paper reports the use of lux-marked Escherichia coli (selected as a general indicator) and lux-marked pseudomonads (selected for site relevance) to assess the toxicity of soils and groundwater at a BTEX contaminated site. This approach, coupled with a regime of sample manipulation, allows the determination of the nature of the toxicity as well as providing information on which to base a remediation strategy.

AB - A toxicity survey can provide invaluable information prior to managed bioremediation of industrially contaminated sites. Furthermore, toxicity monitoring is often required during site remediation to monitor progress. Conventional toxicity assessment involves analytical techniques such as spectrometry, chromatography (HPLC and GC), CC-mass spectrometry and atomic absorption techniques for the determination of heavy metals. These techniques are powerful and sensitive to ppm and ppb levels. However, the ability to detect a compound does not provide information regarding the biological effects of that compound, its persistence in the environment, or information that can be employed in the design of a remediation strategy, lux-marked bacterial biosensors have been found to be rapid and sensitive indicators of toxicity of a wide range of organic and inorganic pollutants. This paper reports the use of lux-marked Escherichia coli (selected as a general indicator) and lux-marked pseudomonads (selected for site relevance) to assess the toxicity of soils and groundwater at a BTEX contaminated site. This approach, coupled with a regime of sample manipulation, allows the determination of the nature of the toxicity as well as providing information on which to base a remediation strategy.

KW - PSEUDOMONAS-FLUORESCENS

KW - LUMINESCENCE

M3 - Conference contribution

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BT - In Situ Bioremediation of Petroleum Hydrocarbon and Other Organic Compounds

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A2 - Leeson, A

PB - Battelle Press

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