Protective immunity against pulmonary cryptococcosis is associated with STAT1-mediated classical macrophage activation

Sarah E. Hardison, Gina Herrera, Mattie L. Young, Camaron R. Hole, Karen L. Wozniak, Floyd L. Wormley*

*Corresponding author for this work

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Experimental pulmonary Cryptococcus neoformans infection in BALB/c mice is associated with polarized Th2-type cytokine production, alternative macrophage activation, and severe bronchopneumonia. In contrast, pulmonary infection with a C. neoformans strain that secretes IFN-γ, H99γ, elicits Th1-type cytokine production and classical macrophage activation. Additionally, mice infected with H99γ resolve the acute infection and are subsequently protected against challenge with wild-type C. neoformans. The present study characterizes macrophage activation during the protective response to wild-type C. neoformans in mice previously immunized with H99γ. We observed increased pulmonary Th1-type cytokine production in lung homogenates and classical macrophage activation as evidenced by enhanced expression of inducible NO synthase in the lungs of H99γ-immunized mice compared with mice given a nonprotective immunization with heat-killed C. neoformans (HKCn). Furthermore, macrophages isolated from H99γ-immunized mice on day 7 postchallenge and cultured in vitro were fungistatic against C. neoformans, whereas cryptococcal growth was uncontrolled within macrophages from HKCn-immunized mice. Th2-type cytokine production and induction of alternatively activated macrophages were also observed in lungs of HKCn-immunized mice during rechallenge. Gene expression arrays showed that classical macrophage activation during challenge infection in H99γ-immunized mice was associated with induction of the transcription factor STAT1 and its downstream targets IFN regulatory factor-1, suppressor of cytokine signaling-1, CXCL9, and CXCL10. These studies demonstrate that protective responses to C. neoformans challenge in immunized mice include classical macrophage activation and enhanced macrophage fungistasis of C. neoformans yeasts. Finally, the classical activation phenotype of protective anticryptococcal macrophages is likely mediated via STAT1 signal transduction pathways.
Original languageEnglish
Pages (from-to)4060-4068
Number of pages9
JournalThe Journal of Immunology
Volume189
Issue number8
Early online date14 Sep 2012
DOIs
Publication statusPublished - 15 Oct 2012

Keywords

  • animals
  • cells, cultured
  • cryptococcosis
  • Cryptococcus neoformans
  • disease resistance
  • female
  • immunophenotyping
  • lung diseases, fungal
  • macrophage activation
  • mice
  • mice, inbred BALB C
  • STAT1 transcription factor
  • signal transduction

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