Proteomic changes in the crucian carp brain during exposure to anoxia

Richard W Smith, Phil Cash, Stian Ellefsen, Goran E Nilsson

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

During exposure to anoxia, the crucian carp brain is able to maintain normal overall protein synthesis rates. However, it is not known if there are alterations in the synthesis or expression of specific proteins. This investigation addresses this issue by comparing the normoxic and anoxic brain proteome. Nine proteins were found to be reduced by anoxia. Reductions in the glycolytic pathway proteins creatine kinase, fructose biphosphate aldolase, glyceraldehyde-3-phosphate dehydrogenase, triosephosphate isomerase and lactate dehydrogenase reflect the reduced production and requirement for adenosine tri-phosphate during anoxia. In terms of neural protection, voltage-dependent anion channel, a protein associated with neuronal apoptosis, was reduced, along with gefiltin, a protein associated with the subsequent need for neuronal repair. Additionally the expression of proteins associated with neural degeneration and impaired cognitive function also declined; dihydropyrimidinase-like protein-3 and vesicle amine transport protein-1. One protein was found to be increased by anoxia; pre-proependymin, the precursor to ependymin. Ependymin fulfils multiple roles in neural plasticity, memory formation and learning, neuron growth and regeneration, and is able to reverse the possibility of apoptosis, thus further protecting the anoxic brain
Original languageEnglish
Pages (from-to)2217-2229
Number of pages13
JournalProteomics
Volume9
Issue number8
Early online date25 Mar 2009
DOIs
Publication statusPublished - 8 Apr 2009

Fingerprint

Carps
Proteomics
Brain
Proteins
dihydropyrimidinase
Voltage-Dependent Anion Channels
Triose-Phosphate Isomerase
Apoptosis
Fructose-Bisphosphate Aldolase
Glyceraldehyde-3-Phosphate Dehydrogenases
Neuronal Plasticity
Adenine Nucleotides
Hypoxia
Proteome
Creatine Kinase
L-Lactate Dehydrogenase
Protein Kinases
Cognition
Amines
Regeneration

Keywords

  • proteomics
  • brain
  • crucian carp
  • cognitive function
  • ependymin
  • glycolytic pathway
  • neural degeneration
  • neuronal apoptosis

Cite this

Proteomic changes in the crucian carp brain during exposure to anoxia. / Smith, Richard W; Cash, Phil; Ellefsen, Stian; Nilsson, Goran E.

In: Proteomics, Vol. 9, No. 8, 08.04.2009, p. 2217-2229.

Research output: Contribution to journalArticle

Smith, Richard W ; Cash, Phil ; Ellefsen, Stian ; Nilsson, Goran E. / Proteomic changes in the crucian carp brain during exposure to anoxia. In: Proteomics. 2009 ; Vol. 9, No. 8. pp. 2217-2229.
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