Proteomic profiling of liver from Atlantic salmon (Salmo salar) fed genetically modified soy compared to the near-isogenic non-GM Line

Nini H Sissener, Samuel A M Martin, Phillip Cash, Ernst M Hevrøy, Monica Sanden, Gro-Ingunn Hemre

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The aim of this study was to investigate potential differences in liver protein expression of Atlantic salmon fed genetically modified (GM) Roundup Ready(R) soy at a high inclusion level (25% inclusion, constituting 21% of crude protein in the diet) for 7 months or a compositionally similar non-GM diet. The liver was selected as the target organ due to its importance in the general metabolism, and 2D gel electrophoresis used as a screening tool. Samples from 12 individual fish from each diet group were evaluated. Of a total of 781 analysed protein spots, only 36 were significantly different by ANOVA (p <0.05) in abundance between the diet groups. All these spots had low fold differences (1.2-1.6) and high false discovery rate (q = 0.44), indicating minor differences in liver protein synthesis between fish fed GM and non-GM soy. Additionally, low fold differences were observed. Four protein spots were analyzed by liquid chromatography tandem mass spectrometry and identified using a combination of online searches in NCBI and searches in an inhouse database containing salmonid expressed sequence tags and contigs. Follow-up on these proteins by real-time polymerase chain reaction did not identify differences at the transcriptional level.
Original languageEnglish
Pages (from-to)273-281
Number of pages9
JournalMarine Biotechnology
Volume12
Issue number3
Early online date17 Jul 2009
DOIs
Publication statusPublished - Jun 2010

Fingerprint

Salmo salar
proteomics
Proteomics
liver protein
liver
protein
Liver
diet
Diet
Proteins
glyphosate
protein synthesis
proteins
Fishes
expressed sequence tags
fish feeds
gel electrophoresis
liquid chromatography
fold
quantitative polymerase chain reaction

Keywords

  • Atlantic salmon
  • biotechnology
  • genetic modification
  • liver
  • roundup ready
  • soybean

Cite this

Proteomic profiling of liver from Atlantic salmon (Salmo salar) fed genetically modified soy compared to the near-isogenic non-GM Line. / Sissener, Nini H; Martin, Samuel A M; Cash, Phillip; Hevrøy, Ernst M; Sanden, Monica; Hemre, Gro-Ingunn.

In: Marine Biotechnology, Vol. 12, No. 3, 06.2010, p. 273-281.

Research output: Contribution to journalArticle

Sissener, Nini H ; Martin, Samuel A M ; Cash, Phillip ; Hevrøy, Ernst M ; Sanden, Monica ; Hemre, Gro-Ingunn. / Proteomic profiling of liver from Atlantic salmon (Salmo salar) fed genetically modified soy compared to the near-isogenic non-GM Line. In: Marine Biotechnology. 2010 ; Vol. 12, No. 3. pp. 273-281.
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