Quantification of late blight resistance of potato using transgenic Phytophthora infestans expressing beta-glucuronidase.

S Kamoun, West Pvan, F Govers

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

A transgenic Phytophthora infestans strain that constitutively produces and secretes β-glucuronidase (GUS) was used in detached leaf assays to quantify the levels of resistance to late blight in potato cultivars Surprise, Irene, Pimpernel, Alpha and Bintje. Four days after inoculation levels of GUS activity were determined in infected leaves. Significant differences between the various cultivars were observed. Discrimination between resistant and susceptible cultivars was possible based solely on levels of GUS activity. Regression analysis revealed a positive correlation between in planta GUS levels and field resistance expressed as Area under Disease Progress Curve (ADPC).
Original languageEnglish
Pages (from-to)521-525
Number of pages5
JournalEuropean journal of plant pathology.
Volume104
Issue number5
DOIs
Publication statusPublished - Jul 1998

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Phytophthora infestans
beta-glucuronidase
genetically modified organisms
potatoes
cultivars
Plantae
leaves
regression analysis
assays

Cite this

Quantification of late blight resistance of potato using transgenic Phytophthora infestans expressing beta-glucuronidase. / Kamoun, S; Pvan, West; Govers, F.

In: European journal of plant pathology., Vol. 104, No. 5, 07.1998, p. 521-525.

Research output: Contribution to journalArticle

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abstract = "A transgenic Phytophthora infestans strain that constitutively produces and secretes β-glucuronidase (GUS) was used in detached leaf assays to quantify the levels of resistance to late blight in potato cultivars Surprise, Irene, Pimpernel, Alpha and Bintje. Four days after inoculation levels of GUS activity were determined in infected leaves. Significant differences between the various cultivars were observed. Discrimination between resistant and susceptible cultivars was possible based solely on levels of GUS activity. Regression analysis revealed a positive correlation between in planta GUS levels and field resistance expressed as Area under Disease Progress Curve (ADPC).",
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