Quantification of late blight resistance of potato using transgenic Phytophthora infestans expressing beta-glucuronidase.

S Kamoun; P van West; F Govers

doi:10.1023/a:1008698620906

Quantification of late blight resistance of potato using transgenic Phytophthora infestans expressing beta-glucuronidase.

S Kamoun, P van West, F Govers

Medical Sciences

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27 Citations (Scopus)

Abstract

A transgenic Phytophthora infestans strain that constitutively produces and secretes β-glucuronidase (GUS) was used in detached leaf assays to quantify the levels of resistance to late blight in potato cultivars Surprise, Irene, Pimpernel, Alpha and Bintje. Four days after inoculation levels of GUS activity were determined in infected leaves. Significant differences between the various cultivars were observed. Discrimination between resistant and susceptible cultivars was possible based solely on levels of GUS activity. Regression analysis revealed a positive correlation between in planta GUS levels and field resistance expressed as Area under Disease Progress Curve (ADPC).

Original language	English
Pages (from-to)	521-525
Number of pages	5
Journal	European journal of plant pathology.
Volume	104
Issue number	5
DOIs	https://doi.org/10.1023/a:1008698620906
Publication status	Published - Jul 1998

Bibliographical note

Acknowledgements
We thank Howard Judelson for giving us plasmids pHAMT35G and pTH209, Leontine Colon for providing us with the potato cultivars, John van’t Klooster
for help with the fluorometric assay, Wim Blok for help with the figures and Pierre de Wit for critically reading the manuscript. This work was supported by the European Commission, project AIR2-CT94-1263. P. van West was supported by NWO-SLW project 805-22.461.

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@article{32694eddfa994205afa552ae7d77eb1d,

title = "Quantification of late blight resistance of potato using transgenic Phytophthora infestans expressing beta-glucuronidase.",

abstract = "A transgenic Phytophthora infestans strain that constitutively produces and secretes β-glucuronidase (GUS) was used in detached leaf assays to quantify the levels of resistance to late blight in potato cultivars Surprise, Irene, Pimpernel, Alpha and Bintje. Four days after inoculation levels of GUS activity were determined in infected leaves. Significant differences between the various cultivars were observed. Discrimination between resistant and susceptible cultivars was possible based solely on levels of GUS activity. Regression analysis revealed a positive correlation between in planta GUS levels and field resistance expressed as Area under Disease Progress Curve (ADPC).",

author = "S Kamoun and {van West}, P and F Govers",

note = "Acknowledgements We thank Howard Judelson for giving us plasmids pHAMT35G and pTH209, Leontine Colon for providing us with the potato cultivars, John van{\textquoteright}t Klooster for help with the fluorometric assay, Wim Blok for help with the figures and Pierre de Wit for critically reading the manuscript. This work was supported by the European Commission, project AIR2-CT94-1263. P. van West was supported by NWO-SLW project 805-22.461. ",

year = "1998",

month = jul,

doi = "10.1023/a:1008698620906",

language = "English",

volume = "104",

pages = "521--525",

journal = "European journal of plant pathology.",

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T1 - Quantification of late blight resistance of potato using transgenic Phytophthora infestans expressing beta-glucuronidase.

AU - Kamoun, S

AU - van West, P

AU - Govers, F

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PY - 1998/7

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N2 - A transgenic Phytophthora infestans strain that constitutively produces and secretes β-glucuronidase (GUS) was used in detached leaf assays to quantify the levels of resistance to late blight in potato cultivars Surprise, Irene, Pimpernel, Alpha and Bintje. Four days after inoculation levels of GUS activity were determined in infected leaves. Significant differences between the various cultivars were observed. Discrimination between resistant and susceptible cultivars was possible based solely on levels of GUS activity. Regression analysis revealed a positive correlation between in planta GUS levels and field resistance expressed as Area under Disease Progress Curve (ADPC).

AB - A transgenic Phytophthora infestans strain that constitutively produces and secretes β-glucuronidase (GUS) was used in detached leaf assays to quantify the levels of resistance to late blight in potato cultivars Surprise, Irene, Pimpernel, Alpha and Bintje. Four days after inoculation levels of GUS activity were determined in infected leaves. Significant differences between the various cultivars were observed. Discrimination between resistant and susceptible cultivars was possible based solely on levels of GUS activity. Regression analysis revealed a positive correlation between in planta GUS levels and field resistance expressed as Area under Disease Progress Curve (ADPC).

UR - http://europepmc.org/abstract/AGR/IND21637822

U2 - 10.1023/a:1008698620906

DO - 10.1023/a:1008698620906

M3 - Article

VL - 104

SP - 521

EP - 525

JO - European journal of plant pathology.

JF - European journal of plant pathology.

IS - 5

ER -

Quantification of late blight resistance of potato using transgenic Phytophthora infestans expressing beta-glucuronidase.

Abstract

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