Rapid selection of anti-hapten antibodies isolated from synthetic and semi-synthetic antibody phage display libraries expressed in Escherichia coli

Gillian Louise Strachan, J. McElhiney, Matthew Ross Drever, F. McIntosh, L. A. Lawton, Andrew Justin Radcliffe Porter

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Antibody phage display libraries (Griffin and Tomlinson I) displaying antibody genes and maintained and amplified in Escherichia coli were used to isolate antibodies to the hapten target microcystin LR (1000 Da) conjugated to either bovine serum albumin or keyhole limpet haemocyanin. In competition enzyme-linked immunosorbent assay, bacterially expressed antibodies selected via the Griffin library showed at least 300 times greater sensitivity than those isolated from the Tomlinson library, for free microcystin. Bacterially expressed phage antibody libraries provide a rapid and relatively easy route for the selection of monoclonal antibodies specific for even the most difficult of antigenic targets such as free haptens. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

Original languageEnglish
Pages (from-to)257-261
Number of pages4
JournalFEMS Microbiology Letters
Volume210
Issue number2
DOIs
Publication statusPublished - 2002

Keywords

  • phage display
  • hapten
  • antibody fragment
  • immunoassay
  • MICROCYSTINS
  • FRAGMENTS
  • QUANTIFICATION

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