Regeneration of corneal cells and nerves in an implanted collagen corneal substitute

C. R. McLaughlin, P. Fagerholm, L. Muzakare, N. Lagali, John Vincent Forrester, Lucia Kuffova, M. A. Rafat, Y. Liu, N. Shinozaki, S. G. Vascotto, R. Munger, M. Griffith

Research output: Contribution to journalArticle

24 Citations (Scopus)
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Abstract

Purpose: Our objective was to evaluate promotion of tissue regeneration by extracellular matrix (ECM) mimics, by using corneal implantation as a model system.

Methods: Carbodiimide cross-linked porcine type I collagen was molded into appropriate corneal dimensions to serve as substitutes for natural corneal ECM. These were implanted into corneas of mini-pigs after removal of the host tissue, and tracked over 12 months, by clinical examination, slit-lamp biomicroscopy, in vivo confocal microscopy, topography, and esthesiometry. Histopathology and tensile strength testing were performed at the end of 12 months. Other samples were biotin labeled and implanted into mice to evaluate matrix remodeling.

Results: The implants promoted regeneration of corneal cells, nerves, and the tear film while retaining optical clarity. Mechanical testing data were consistent with stable, seamless host-graft integration in regenerated corneas, which were as robust as the untreated fellow corneas. Biotin conjugation is an effective method for tracking the implant within the host tissue.

Conclusions: We show that a simple ECM mimetic can promote regeneration of corneal cells and nerves. Gradual turnover of matrix material as part of the natural remodeling process allowed for stable integration with host tissue and restoration of mechanical properties of the organ. The simplicity in fabrication and shown functionality shows potential for ECM substitutes in future clinical applications.

Original languageEnglish
Pages (from-to)580-589
Number of pages9
JournalCornea
Volume27
Issue number5
DOIs
Publication statusPublished - Jun 2008

Keywords

  • cornea
  • extracellular matrix
  • collagen
  • nerve regeneration
  • mechanical proper-ties
  • hydrogel
  • vivo confocal microscopy
  • biomechanical properties
  • porcine cornea
  • full-thickness
  • matrix
  • membrane
  • grafts
  • mouse
  • model

Cite this

McLaughlin, C. R., Fagerholm, P., Muzakare, L., Lagali, N., Forrester, J. V., Kuffova, L., ... Griffith, M. (2008). Regeneration of corneal cells and nerves in an implanted collagen corneal substitute. Cornea, 27(5), 580-589. https://doi.org/10.1097/ICO.0b013e3181658408

Regeneration of corneal cells and nerves in an implanted collagen corneal substitute. / McLaughlin, C. R.; Fagerholm, P.; Muzakare, L.; Lagali, N.; Forrester, John Vincent; Kuffova, Lucia; Rafat, M. A.; Liu, Y.; Shinozaki, N.; Vascotto, S. G.; Munger, R.; Griffith, M.

In: Cornea, Vol. 27, No. 5, 06.2008, p. 580-589.

Research output: Contribution to journalArticle

McLaughlin, CR, Fagerholm, P, Muzakare, L, Lagali, N, Forrester, JV, Kuffova, L, Rafat, MA, Liu, Y, Shinozaki, N, Vascotto, SG, Munger, R & Griffith, M 2008, 'Regeneration of corneal cells and nerves in an implanted collagen corneal substitute', Cornea, vol. 27, no. 5, pp. 580-589. https://doi.org/10.1097/ICO.0b013e3181658408
McLaughlin CR, Fagerholm P, Muzakare L, Lagali N, Forrester JV, Kuffova L et al. Regeneration of corneal cells and nerves in an implanted collagen corneal substitute. Cornea. 2008 Jun;27(5):580-589. https://doi.org/10.1097/ICO.0b013e3181658408
McLaughlin, C. R. ; Fagerholm, P. ; Muzakare, L. ; Lagali, N. ; Forrester, John Vincent ; Kuffova, Lucia ; Rafat, M. A. ; Liu, Y. ; Shinozaki, N. ; Vascotto, S. G. ; Munger, R. ; Griffith, M. / Regeneration of corneal cells and nerves in an implanted collagen corneal substitute. In: Cornea. 2008 ; Vol. 27, No. 5. pp. 580-589.
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AU - Kuffova, Lucia

AU - Rafat, M. A.

AU - Liu, Y.

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