Relaxometric investigations addressing the determination of intracellular water lifetime: a novel tumour biomarker of general applicability

Maria Rosaria Ruggiero; Simona Baroni; Silvio Aime; Simonetta Geninatti Crich

doi:10.1080/00268976.2018.1527045

Relaxometric investigations addressing the determination of intracellular water lifetime: a novel tumour biomarker of general applicability

Maria Rosaria Ruggiero, Simona Baroni, Silvio Aime, Simonetta Geninatti Crich^* (Corresponding Author)

^*Corresponding author for this work

Applied Medicine

Research output: Contribution to journal › Article › peer-review

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Abstract

¹ H Fast-Field Cycling NMR relaxometry is proposed as a powerful method to investigate tumour cell metabolism by measuring changes in cell water content and mobility across the cellular membrane. Measurements of intracellular water residence time in cultured cells were carried out by measuring T ₁ at fixed field (0.2 T) after the addition of a paramagnetic Gd complex (Prohance) at different concentrations in the external medium. Investigations on tumour cells (mammary adenocarcinoma TS/A) grown in normo- or hypoxic conditions or suspended in ‘hypo-osmotic’ solutions allowed us to demonstrate that both hypoxic and hypo-osmotic conditions cause a marked increase in water mobility as assessed by the elongation of T ₁ . Conversely, the metabolic change caused by glutamine (an aminoacid essential for tumour growth) deprivation caused a water mobility decrease (shorter T ₁ ). These findings suggest that T ₁ measurements at low and variable magnetic field strengths, giving access to the assessment of intracellular water lifetime, can provide important information about tumour cell metabolism in real time and non-invasively.

Original language	English
Pages (from-to)	968-974
Number of pages	7
Journal	Molecular Physics
Volume	117
Issue number	7-8
DOIs	https://doi.org/10.1080/00268976.2018.1527045
Publication status	Published - 25 Sept 2018

Bibliographical note

This project has received funding from the European Union’s Horizon 2020 (H2020 Health) research and innovation programme under grant agreement No 668119 (project ‘IDentIFY’). This article is based upon work from COST Action EURELAX, CA15209, supported by COST (European Cooperation in Science and Technology).

Keywords

intracellular water lifetime
Nuclear Magnetic Resonance Profiles (NMRD)
relaxometry
tumour hypoxia
tumour metabolism

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10.1080/00268976.2018.1527045Licence: CC BY

Ruggiero_etal_MP_Relaxometric_investigations_VOR
© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Final published version, 1.92 MBLicence: CC BY

Cite this

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title = "Relaxometric investigations addressing the determination of intracellular water lifetime: a novel tumour biomarker of general applicability",

abstract = "1 H Fast-Field Cycling NMR relaxometry is proposed as a powerful method to investigate tumour cell metabolism by measuring changes in cell water content and mobility across the cellular membrane. Measurements of intracellular water residence time in cultured cells were carried out by measuring T 1 at fixed field (0.2 T) after the addition of a paramagnetic Gd complex (Prohance) at different concentrations in the external medium. Investigations on tumour cells (mammary adenocarcinoma TS/A) grown in normo- or hypoxic conditions or suspended in {\textquoteleft}hypo-osmotic{\textquoteright} solutions allowed us to demonstrate that both hypoxic and hypo-osmotic conditions cause a marked increase in water mobility as assessed by the elongation of T 1 . Conversely, the metabolic change caused by glutamine (an aminoacid essential for tumour growth) deprivation caused a water mobility decrease (shorter T 1 ). These findings suggest that T 1 measurements at low and variable magnetic field strengths, giving access to the assessment of intracellular water lifetime, can provide important information about tumour cell metabolism in real time and non-invasively. ",

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N2 - 1 H Fast-Field Cycling NMR relaxometry is proposed as a powerful method to investigate tumour cell metabolism by measuring changes in cell water content and mobility across the cellular membrane. Measurements of intracellular water residence time in cultured cells were carried out by measuring T 1 at fixed field (0.2 T) after the addition of a paramagnetic Gd complex (Prohance) at different concentrations in the external medium. Investigations on tumour cells (mammary adenocarcinoma TS/A) grown in normo- or hypoxic conditions or suspended in ‘hypo-osmotic’ solutions allowed us to demonstrate that both hypoxic and hypo-osmotic conditions cause a marked increase in water mobility as assessed by the elongation of T 1 . Conversely, the metabolic change caused by glutamine (an aminoacid essential for tumour growth) deprivation caused a water mobility decrease (shorter T 1 ). These findings suggest that T 1 measurements at low and variable magnetic field strengths, giving access to the assessment of intracellular water lifetime, can provide important information about tumour cell metabolism in real time and non-invasively.

AB - 1 H Fast-Field Cycling NMR relaxometry is proposed as a powerful method to investigate tumour cell metabolism by measuring changes in cell water content and mobility across the cellular membrane. Measurements of intracellular water residence time in cultured cells were carried out by measuring T 1 at fixed field (0.2 T) after the addition of a paramagnetic Gd complex (Prohance) at different concentrations in the external medium. Investigations on tumour cells (mammary adenocarcinoma TS/A) grown in normo- or hypoxic conditions or suspended in ‘hypo-osmotic’ solutions allowed us to demonstrate that both hypoxic and hypo-osmotic conditions cause a marked increase in water mobility as assessed by the elongation of T 1 . Conversely, the metabolic change caused by glutamine (an aminoacid essential for tumour growth) deprivation caused a water mobility decrease (shorter T 1 ). These findings suggest that T 1 measurements at low and variable magnetic field strengths, giving access to the assessment of intracellular water lifetime, can provide important information about tumour cell metabolism in real time and non-invasively.

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Relaxometric investigations addressing the determination of intracellular water lifetime: a novel tumour biomarker of general applicability

Abstract

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Keywords

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