Restricted patterns of Hoxd10 and Hoxd11 set segmental differences in motoneuron subtype complement in the lumbosacral spinal cord

Mala Misra, Veeral Shah, Ellen Carpenter, Peter McCaffery, Cynthia Lance-Jones

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

During normal vertebrate development, Hoxd10 and Hoxd11 are expressed by differentiating motoneurons in restricted patterns along the rostrocaudal axis of the lumbosacral (LS) spinal cord. To assess the roles of these genes in the attainment of motoneuron subtypes characteristic of LS subdomains, we examined subtype complement after overexpression of Hoxd10 or Hoxd11 in the embryonic chick LS cord and in a Hoxd10 loss-of-function mouse embryo. Data presented here provide evidence that Hoxd10 defines the position of the lateral motor column (LMC) as a whole and, in rostral LS segments, specifically promotes the development of motoneurons of the lateral subdivision of the lateral motor column (LMCl). In contrast, Hoxd11 appears to impart a caudal and medial LMC (LMCm) identity to some motoneurons and molecular profiles suggestive of a suppression of LMC development in others. We also provide evidence that Hoxd11 suppresses the expression of Hoxd10 and the retinoic acid synthetic enzyme, retinaldehyde dehydrogenase 2 (RALDH2). In a normal chick embryo, Hoxd10 and RALDH2 are expressed throughout the LS region at early stages of motoneuron differentiation but their levels decline in Hoxd11-expressing caudal LS segments that ultimately contain few LMCl motoneurons. We hypothesize that one of the roles played by Hoxd11 is to modulate Hoxd10 and local retinoic acid levels and thus, perhaps define the caudal boundaries of the LMC and its subtype complement.
Original languageEnglish
Pages (from-to)54-72
Number of pages19
JournalDevelopmental Biology
Volume330
Issue number1
DOIs
Publication statusPublished - 1 Jun 2009

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Motor Neurons
Spinal Cord
Retinaldehyde
Tretinoin
Oxidoreductases
Lumbosacral Region
Chick Embryo
Vertebrates
Embryonic Structures
Enzymes
Genes

Keywords

  • neural tube development
  • LIM genes
  • RALDH2
  • lateral motor column
  • pattern formation
  • fate determination
  • motor-neuron identity
  • peripheral nervous-system
  • progenitor-cell identity
  • lim homeobox genes
  • targeted disruption
  • retinoic acid
  • homeodomain proteins
  • pool identity
  • retinaldehyde dehydrogenase-2
  • transcriptional codes

Cite this

Restricted patterns of Hoxd10 and Hoxd11 set segmental differences in motoneuron subtype complement in the lumbosacral spinal cord. / Misra, Mala; Shah, Veeral; Carpenter, Ellen; McCaffery, Peter; Lance-Jones, Cynthia.

In: Developmental Biology, Vol. 330, No. 1, 01.06.2009, p. 54-72.

Research output: Contribution to journalArticle

Misra, Mala ; Shah, Veeral ; Carpenter, Ellen ; McCaffery, Peter ; Lance-Jones, Cynthia. / Restricted patterns of Hoxd10 and Hoxd11 set segmental differences in motoneuron subtype complement in the lumbosacral spinal cord. In: Developmental Biology. 2009 ; Vol. 330, No. 1. pp. 54-72.
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T1 - Restricted patterns of Hoxd10 and Hoxd11 set segmental differences in motoneuron subtype complement in the lumbosacral spinal cord

AU - Misra, Mala

AU - Shah, Veeral

AU - Carpenter, Ellen

AU - McCaffery, Peter

AU - Lance-Jones, Cynthia

PY - 2009/6/1

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N2 - During normal vertebrate development, Hoxd10 and Hoxd11 are expressed by differentiating motoneurons in restricted patterns along the rostrocaudal axis of the lumbosacral (LS) spinal cord. To assess the roles of these genes in the attainment of motoneuron subtypes characteristic of LS subdomains, we examined subtype complement after overexpression of Hoxd10 or Hoxd11 in the embryonic chick LS cord and in a Hoxd10 loss-of-function mouse embryo. Data presented here provide evidence that Hoxd10 defines the position of the lateral motor column (LMC) as a whole and, in rostral LS segments, specifically promotes the development of motoneurons of the lateral subdivision of the lateral motor column (LMCl). In contrast, Hoxd11 appears to impart a caudal and medial LMC (LMCm) identity to some motoneurons and molecular profiles suggestive of a suppression of LMC development in others. We also provide evidence that Hoxd11 suppresses the expression of Hoxd10 and the retinoic acid synthetic enzyme, retinaldehyde dehydrogenase 2 (RALDH2). In a normal chick embryo, Hoxd10 and RALDH2 are expressed throughout the LS region at early stages of motoneuron differentiation but their levels decline in Hoxd11-expressing caudal LS segments that ultimately contain few LMCl motoneurons. We hypothesize that one of the roles played by Hoxd11 is to modulate Hoxd10 and local retinoic acid levels and thus, perhaps define the caudal boundaries of the LMC and its subtype complement.

AB - During normal vertebrate development, Hoxd10 and Hoxd11 are expressed by differentiating motoneurons in restricted patterns along the rostrocaudal axis of the lumbosacral (LS) spinal cord. To assess the roles of these genes in the attainment of motoneuron subtypes characteristic of LS subdomains, we examined subtype complement after overexpression of Hoxd10 or Hoxd11 in the embryonic chick LS cord and in a Hoxd10 loss-of-function mouse embryo. Data presented here provide evidence that Hoxd10 defines the position of the lateral motor column (LMC) as a whole and, in rostral LS segments, specifically promotes the development of motoneurons of the lateral subdivision of the lateral motor column (LMCl). In contrast, Hoxd11 appears to impart a caudal and medial LMC (LMCm) identity to some motoneurons and molecular profiles suggestive of a suppression of LMC development in others. We also provide evidence that Hoxd11 suppresses the expression of Hoxd10 and the retinoic acid synthetic enzyme, retinaldehyde dehydrogenase 2 (RALDH2). In a normal chick embryo, Hoxd10 and RALDH2 are expressed throughout the LS region at early stages of motoneuron differentiation but their levels decline in Hoxd11-expressing caudal LS segments that ultimately contain few LMCl motoneurons. We hypothesize that one of the roles played by Hoxd11 is to modulate Hoxd10 and local retinoic acid levels and thus, perhaps define the caudal boundaries of the LMC and its subtype complement.

KW - neural tube development

KW - LIM genes

KW - RALDH2

KW - lateral motor column

KW - pattern formation

KW - fate determination

KW - motor-neuron identity

KW - peripheral nervous-system

KW - progenitor-cell identity

KW - lim homeobox genes

KW - targeted disruption

KW - retinoic acid

KW - homeodomain proteins

KW - pool identity

KW - retinaldehyde dehydrogenase-2

KW - transcriptional codes

U2 - 10.1016/j.ydbio.2009.03.009

DO - 10.1016/j.ydbio.2009.03.009

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VL - 330

SP - 54

EP - 72

JO - Developmental Biology

JF - Developmental Biology

SN - 0012-1606

IS - 1

ER -