RNA-sequencing elucidates the regulation of behavioural transitions associated with mating in honey bee queens

Fabio Manfredini; Mark J F Brown; Vanina Vergoz; Ben Oldroyd

doi:10.1186/s12864-015-1750-7

RNA-sequencing elucidates the regulation of behavioural transitions associated with mating in honey bee queens

Fabio Manfredini (Corresponding Author), Mark J F Brown (Corresponding Author), Vanina Vergoz, Ben Oldroyd

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Abstract

Mating is a complex process, which is frequently associated with behavioural and physiological changes. However, understanding of the genetic underpinnings of these changes is limited. Honey bees are both a model system in behavioural genomics, and the dominant managed pollinator of human crops; consequently understanding the mating process has both pure and applied value. We used next-generation transcriptomics to probe changes in gene expression in the brains of honey bee queens, as they transition from virgin to mated reproductive status. In addition, we used CO 2 -narcosis, which induces oviposition without mating, to isolate the process of reproductive maturation.

Original language	English
Article number	563
Number of pages	13
Journal	BMC Genomics
Volume	16
DOIs	https://doi.org/10.1186/s12864-015-1750-7
Publication status	Published - 31 Jul 2015

Keywords

Apis mellifera
Australian honey bees
Behaviour
Brain
Mating
Carbon dioxide
Double necrosis
Transcriptomics
RNAseq
Gene expression

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10.1186/s12864-015-1750-7Licence: CC BY

Manfredini_BMCG_RNA_VOR
© 2015 Manfredini et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Final published version, 1.59 MBLicence: CC BY

https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?token=oludywiunlwblgd&acc=GSE65833Licence: Unspecified

Fabio Manfredini
- Biological Sciences, Aberdeen Centre For Environmental Sustainability - Lecturer
Person: Academic

Cite this

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title = "RNA-sequencing elucidates the regulation of behavioural transitions associated with mating in honey bee queens",

abstract = "Mating is a complex process, which is frequently associated with behavioural and physiological changes. However, understanding of the genetic underpinnings of these changes is limited. Honey bees are both a model system in behavioural genomics, and the dominant managed pollinator of human crops; consequently understanding the mating process has both pure and applied value. We used next-generation transcriptomics to probe changes in gene expression in the brains of honey bee queens, as they transition from virgin to mated reproductive status. In addition, we used CO 2 -narcosis, which induces oviposition without mating, to isolate the process of reproductive maturation.",

keywords = "Apis mellifera, Australian honey bees, Behaviour, Brain, Mating, Carbon dioxide, Double necrosis, Transcriptomics, RNAseq, Gene expression",

author = "Fabio Manfredini and Brown, {Mark J F} and Vanina Vergoz and Ben Oldroyd",

note = "This study was funded by a BBSRC ISIS grant BB/J019453/1, a Royal Holloway Research Strategy Fund Grant, and a Leverhulme Grant F/07537/AK to MJFB. BPO was supported by Australian Research Council Discovery grants DP150100151 and DP120101915. FM was supported by a Marie Curie International Incoming Fellowship FP7-PEOPLE-2013-IIF-625487 to MJFB. We would like to thank Dave Galbraight (Penn State) and Alberto Paccanaro (RHUL) for support with analysis of RNAseq data and four anonymous reviewers for providing thoughtful insights that helped to improve the manuscript. ",

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AU - Manfredini, Fabio

AU - Brown, Mark J F

AU - Vergoz, Vanina

AU - Oldroyd, Ben

N1 - This study was funded by a BBSRC ISIS grant BB/J019453/1, a Royal Holloway Research Strategy Fund Grant, and a Leverhulme Grant F/07537/AK to MJFB. BPO was supported by Australian Research Council Discovery grants DP150100151 and DP120101915. FM was supported by a Marie Curie International Incoming Fellowship FP7-PEOPLE-2013-IIF-625487 to MJFB. We would like to thank Dave Galbraight (Penn State) and Alberto Paccanaro (RHUL) for support with analysis of RNAseq data and four anonymous reviewers for providing thoughtful insights that helped to improve the manuscript.

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N2 - Mating is a complex process, which is frequently associated with behavioural and physiological changes. However, understanding of the genetic underpinnings of these changes is limited. Honey bees are both a model system in behavioural genomics, and the dominant managed pollinator of human crops; consequently understanding the mating process has both pure and applied value. We used next-generation transcriptomics to probe changes in gene expression in the brains of honey bee queens, as they transition from virgin to mated reproductive status. In addition, we used CO 2 -narcosis, which induces oviposition without mating, to isolate the process of reproductive maturation.

AB - Mating is a complex process, which is frequently associated with behavioural and physiological changes. However, understanding of the genetic underpinnings of these changes is limited. Honey bees are both a model system in behavioural genomics, and the dominant managed pollinator of human crops; consequently understanding the mating process has both pure and applied value. We used next-generation transcriptomics to probe changes in gene expression in the brains of honey bee queens, as they transition from virgin to mated reproductive status. In addition, we used CO 2 -narcosis, which induces oviposition without mating, to isolate the process of reproductive maturation.

KW - Apis mellifera

KW - Australian honey bees

KW - Behaviour

KW - Brain

KW - Mating

KW - Carbon dioxide

KW - Double necrosis

KW - Transcriptomics

KW - RNAseq

KW - Gene expression

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JO - BMC Genomics

JF - BMC Genomics

SN - 1471-2164

M1 - 563

ER -

RNA-sequencing elucidates the regulation of behavioural transitions associated with mating in honey bee queens

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Fabio Manfredini

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