Robo2 is required for Slit-mediated intraretinal axon guidance

Hannah Thompson, William Andrews, John G Parnavelas, Lynda Erskine

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The developing optic pathway has proven one of the most informative model systems for studying mechanisms of axon guidance. The first step in this process is the directed extension of retinal ganglion cell (RGC) axons within the optic fibre layer (OFL) of the retina towards their exit point from the eye, the optic disc. Previously, we have shown that the inhibitory guidance molecules, Slit1 and Slit2, regulate two distinct aspects of intraretinal axon guidance in a region-specific manner. Using knockout mice, we have found that both of these guidance activities are mediated via Robo2. Of the four vertebrate Robos, only Robo1 and Robo2 are expressed by RGCs. In mice lacking robo1 intraretinal axon guidance occurs normally. However, in mice lacking robo2 RGC axons make qualitatively and quantitatively identical intraretinal pathfinding errors to those reported previously in Slit mutants. This demonstrates clearly that, as in other regions of the optic pathway, Robo2 is the major receptor required for intraretinal axon guidance. Furthermore, the results suggest strongly that redundancy with other guidance signals rather than different receptor utilisation is the most likely explanation for the regional specificity of Slit function during intraretinal axon pathfinding.
Original languageEnglish
Pages (from-to)418-426
Number of pages9
JournalDevelopmental Biology
Volume335
Issue number2
Early online date25 Sep 2009
DOIs
Publication statusPublished - 15 Nov 2009

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Retinal Ganglion Cells
Axons
Optic Disk
Knockout Mice
Vertebrates
Retina
Axon Guidance

Keywords

  • animals
  • axons
  • cell polarity
  • in situ hybridization
  • intercellular signaling peptides and proteins
  • mice
  • mice, inbred C57BL
  • nerve tissue proteins
  • receptors, immunologic
  • retinal ganglion cells
  • robo
  • slit
  • axon guidance
  • retinal ganglion cell
  • growth cone
  • retina
  • visual system
  • development

Cite this

Robo2 is required for Slit-mediated intraretinal axon guidance. / Thompson, Hannah; Andrews, William; Parnavelas, John G; Erskine, Lynda.

In: Developmental Biology, Vol. 335, No. 2, 15.11.2009, p. 418-426.

Research output: Contribution to journalArticle

Thompson, Hannah ; Andrews, William ; Parnavelas, John G ; Erskine, Lynda. / Robo2 is required for Slit-mediated intraretinal axon guidance. In: Developmental Biology. 2009 ; Vol. 335, No. 2. pp. 418-426.
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