Root suberin forms an extracellular barrier that affects water relations and mineral nutrition in Arabidopsis

Ivan Baxter, Prashant S Hosmani, Ana Rus, Brett Lahner, Justin O Borevitz, Balasubramaniam Muthukumar, Michael V Mickelbart, Lukas Schreiber, Rochus B Franke, David E Salt

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Abstract

Though central to our understanding of how roots perform their vital function of scavenging water and solutes from the soil, no direct genetic evidence currently exists to support the foundational model that suberin acts to form a chemical barrier limiting the extracellular, or apoplastic, transport of water and solutes in plant roots. Using the newly characterized enhanced suberin1 (esb1) mutant, we established a connection in Arabidopsis thaliana between suberin in the root and both water movement through the plant and solute accumulation in the shoot. Esb1 mutants, characterized by increased root suberin, were found to have reduced day time transpiration rates and increased water-use efficiency during their vegetative growth period. Furthermore, these changes in suberin and water transport were associated with decreases in the accumulation of Ca, Mn, and Zn and increases in the accumulation of Na, S, K, As, Se, and Mo in the shoot. Here, we present direct genetic evidence establishing that suberin in the roots plays a critical role in controlling both water and mineral ion uptake and transport to the leaves. The changes observed in the elemental accumulation in leaves are also interpreted as evidence that a significant component of the radial root transport of Ca, Mn, and Zn occurs in the apoplast.

Original languageEnglish
Article numbere1000492
Number of pages12
JournalPLoS Genetics
Volume5
Issue number5
DOIs
Publication statusPublished - 22 May 2009

Keywords

  • radial hydraulic conductivity
  • Zea-Mays L.
  • apoplastic transport
  • chemical-composition
  • plant-roots
  • identification
  • biosynthesis
  • exodermis
  • acid
  • gene

Cite this

Baxter, I., Hosmani, P. S., Rus, A., Lahner, B., Borevitz, J. O., Muthukumar, B., Mickelbart, M. V., Schreiber, L., Franke, R. B., & Salt, D. E. (2009). Root suberin forms an extracellular barrier that affects water relations and mineral nutrition in Arabidopsis. PLoS Genetics, 5(5), [e1000492]. https://doi.org/10.1371/journal.pgen.1000492