TY - JOUR
T1 - Separation and concentration of bacteria with immobilized antibody fragments
AU - Molloy, P.
AU - Brydon, L.
AU - Porter, A. J.
AU - Harris, W. J.
PY - 1995/1/1
Y1 - 1995/1/1
N2 - P. MOLLOY, L. BRYDON, A.J, PORTER AND W.J. HARRIS. 1995. New methods to quantitatively remove bacteria from food and water samples are required to meet modern safety standards. The recent development of techniques to make Fab/Fv/scFv fragments in bacteria has provided the opportunity to exploit antibodies as specialized chemicals for affinity removal technologies. Single‐chain fragments against Pseudomonas aeruginosa have been expressed in Escherichia coli, purified via a fused poly‐histidine tail and immobilized upon polystyrene beads. The resulting immunoaffinity columns have been shown to effectively remove greater than 90% of an applied 10 million bacteria after a single passage through the column. Column material in the absence of single‐chain retained less than 10% of the bacteria. Pseudomonas were also removed from milk, mixed bacterial cultures and when present at low cell densities.
AB - P. MOLLOY, L. BRYDON, A.J, PORTER AND W.J. HARRIS. 1995. New methods to quantitatively remove bacteria from food and water samples are required to meet modern safety standards. The recent development of techniques to make Fab/Fv/scFv fragments in bacteria has provided the opportunity to exploit antibodies as specialized chemicals for affinity removal technologies. Single‐chain fragments against Pseudomonas aeruginosa have been expressed in Escherichia coli, purified via a fused poly‐histidine tail and immobilized upon polystyrene beads. The resulting immunoaffinity columns have been shown to effectively remove greater than 90% of an applied 10 million bacteria after a single passage through the column. Column material in the absence of single‐chain retained less than 10% of the bacteria. Pseudomonas were also removed from milk, mixed bacterial cultures and when present at low cell densities.
UR - http://www.scopus.com/inward/record.url?scp=0028965190&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2672.1995.tb03418.x
DO - 10.1111/j.1365-2672.1995.tb03418.x
M3 - Article
C2 - 7744720
AN - SCOPUS:0028965190
SN - 0021-8847
VL - 78
SP - 359
EP - 365
JO - Journal of Applied Bacteriology
JF - Journal of Applied Bacteriology
IS - 4
ER -