Separation and concentration of bacteria with immobilized antibody fragments

P. Molloy, L. Brydon, A. J. Porter, W. J. Harris*

*Corresponding author for this work

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

P. MOLLOY, L. BRYDON, A.J, PORTER AND W.J. HARRIS. 1995. New methods to quantitatively remove bacteria from food and water samples are required to meet modern safety standards. The recent development of techniques to make Fab/Fv/scFv fragments in bacteria has provided the opportunity to exploit antibodies as specialized chemicals for affinity removal technologies. Single‐chain fragments against Pseudomonas aeruginosa have been expressed in Escherichia coli, purified via a fused poly‐histidine tail and immobilized upon polystyrene beads. The resulting immunoaffinity columns have been shown to effectively remove greater than 90% of an applied 10 million bacteria after a single passage through the column. Column material in the absence of single‐chain retained less than 10% of the bacteria. Pseudomonas were also removed from milk, mixed bacterial cultures and when present at low cell densities.

Original languageEnglish
Pages (from-to)359-365
Number of pages7
JournalJournal of Applied Bacteriology
Volume78
Issue number4
DOIs
Publication statusPublished - 1 Jan 1995

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

Fingerprint Dive into the research topics of 'Separation and concentration of bacteria with immobilized antibody fragments'. Together they form a unique fingerprint.

  • Cite this