Site-directed genome modification: nucleic acid and protein modules for targeted integration and gene correction

Andreas F Kolb, C J Coates, J M Kaminski, J B Summers, A D Miller, D J Segal

    Research output: Contribution to journalLiterature review

    38 Citations (Scopus)

    Abstract

    A variety of technological advances in recent years have made permanent genetic manipulation of an organism a technical possibility. As the details of natural biological processes for genome modification are elucidated, the enzymes catalyzing these events (transposases, recombinases, integrases and DNA repair enzymes) are being harnessed or modified for the purpose of intentional gene modification. Targeted integration and gene repair can be mediated by the DNA-targeting specificity inherent to a particular enzyme, or rely on user-designed specificities. Integration sites can be defined by using DNA base-pairing or protein-DNA interaction as a means of targeting. This review will describe recent progress in the development of 'user-targetable' systems, particularly highlighting the application of custom DNA-binding proteins or nucleic acid homology to confer specificity..

    Original languageEnglish
    Pages (from-to)399-406
    Number of pages8
    JournalTrends in Biotechnology
    Volume23
    Issue number8
    Early online date27 Jun 2005
    DOIs
    Publication statusPublished - Aug 2005

    Keywords

    • zinc-finger nucleases
    • immunodeficiency-virus type-1
    • group-II introns
    • triplex-forming oligonucleotides
    • DNA-binding proteins
    • transcription factors
    • in-vivo
    • mammalian-cells
    • homologous recombination
    • chimeric nucleases

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