Solid and Liquid Obesogenic Diets Induce Obesity and Counter-Regulatory Changes in Hypothalamic Gene Expression in Juvenile Sprague-Dawley Rats

Zoë A. Archer, Jeremie Corneloup, D. Vernon Rayner, Perry Barrett, Kim-Marie Moar, Julian Mercer

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Contemporary foods and beverages that constitute the diets of adults and children almost certainly contribute to the obesity problem. To develop a model of childhood obesity, we examined the effects of feeding juvenile rats 2 solid diets, either alone or in combination [nonpurified control diet (C), high-energy (HE), or C+HE] with or without the liquid supplement Ensure (EN). Rats were fed C until 4 wk of age and then were assigned to 1 of 6 weight-matched groups that were fed C, HE, C+HE, C+EN, HE+EN, or C+HE+EN for 5 wk. EN accelerated weight gain and increased energy intake and adiposity irrespective of the solid diet consumed. Serum leptin concentrations were increased after the consumption of all diets when compared with C rats, but there was dissociation between leptin levels and adiposity. The type of solid diet had no effect on the expression of a panel of hypothalamic genes except for glutamate-decarboxylase-67. EN decreased mRNA for agouti-related peptide and neuropeptide Y in the arcuate nucleus and DYN in the paraventricular nucleus. Dynorphin and CART mRNA were decreased in the supraoptic retrochiasmatic nucleus. The reduction in orexigenic signaling in the hypothalamus suggests that overconsumption of EN is sensed by the hypothalamus but that any initiated physiological responses fail to compensate effectively and may be negated or overwhelmed by other systems. Providing diets in solid and liquid form, with choice, mimics more closely the human environment. Understanding the interactions between these diets and peripheral and central energy balance systems could be crucial in unraveling the events underlying human obesity and its early development.

Original languageEnglish
Pages (from-to)1483-1490
Number of pages8
JournalThe Journal of Nutrition
Volume137
Issue number6
Publication statusPublished - 1 Jun 2007

Keywords

  • high-energy diet
  • body-weight
  • messenger-RNA
  • food-intake
  • neronal activation
  • induced hyperphagia
  • childhood obesity
  • resistant rats
  • leptin
  • balance

Cite this

Solid and Liquid Obesogenic Diets Induce Obesity and Counter-Regulatory Changes in Hypothalamic Gene Expression in Juvenile Sprague-Dawley Rats. / Archer, Zoë A.; Corneloup, Jeremie; Rayner, D. Vernon; Barrett, Perry; Moar, Kim-Marie; Mercer, Julian.

In: The Journal of Nutrition, Vol. 137, No. 6, 01.06.2007, p. 1483-1490.

Research output: Contribution to journalArticle

@article{f328d6b1c1774556a743abeb36eaa093,
title = "Solid and Liquid Obesogenic Diets Induce Obesity and Counter-Regulatory Changes in Hypothalamic Gene Expression in Juvenile Sprague-Dawley Rats",
abstract = "Contemporary foods and beverages that constitute the diets of adults and children almost certainly contribute to the obesity problem. To develop a model of childhood obesity, we examined the effects of feeding juvenile rats 2 solid diets, either alone or in combination [nonpurified control diet (C), high-energy (HE), or C+HE] with or without the liquid supplement Ensure (EN). Rats were fed C until 4 wk of age and then were assigned to 1 of 6 weight-matched groups that were fed C, HE, C+HE, C+EN, HE+EN, or C+HE+EN for 5 wk. EN accelerated weight gain and increased energy intake and adiposity irrespective of the solid diet consumed. Serum leptin concentrations were increased after the consumption of all diets when compared with C rats, but there was dissociation between leptin levels and adiposity. The type of solid diet had no effect on the expression of a panel of hypothalamic genes except for glutamate-decarboxylase-67. EN decreased mRNA for agouti-related peptide and neuropeptide Y in the arcuate nucleus and DYN in the paraventricular nucleus. Dynorphin and CART mRNA were decreased in the supraoptic retrochiasmatic nucleus. The reduction in orexigenic signaling in the hypothalamus suggests that overconsumption of EN is sensed by the hypothalamus but that any initiated physiological responses fail to compensate effectively and may be negated or overwhelmed by other systems. Providing diets in solid and liquid form, with choice, mimics more closely the human environment. Understanding the interactions between these diets and peripheral and central energy balance systems could be crucial in unraveling the events underlying human obesity and its early development.",
keywords = "high-energy diet, body-weight, messenger-RNA, food-intake, neronal activation, induced hyperphagia, childhood obesity, resistant rats, leptin, balance",
author = "Archer, {Zo{\"e} A.} and Jeremie Corneloup and Rayner, {D. Vernon} and Perry Barrett and Kim-Marie Moar and Julian Mercer",
year = "2007",
month = "6",
day = "1",
language = "English",
volume = "137",
pages = "1483--1490",
journal = "The Journal of Nutrition",
issn = "0022-3166",
publisher = "American Society for Nutrition",
number = "6",

}

TY - JOUR

T1 - Solid and Liquid Obesogenic Diets Induce Obesity and Counter-Regulatory Changes in Hypothalamic Gene Expression in Juvenile Sprague-Dawley Rats

AU - Archer, Zoë A.

AU - Corneloup, Jeremie

AU - Rayner, D. Vernon

AU - Barrett, Perry

AU - Moar, Kim-Marie

AU - Mercer, Julian

PY - 2007/6/1

Y1 - 2007/6/1

N2 - Contemporary foods and beverages that constitute the diets of adults and children almost certainly contribute to the obesity problem. To develop a model of childhood obesity, we examined the effects of feeding juvenile rats 2 solid diets, either alone or in combination [nonpurified control diet (C), high-energy (HE), or C+HE] with or without the liquid supplement Ensure (EN). Rats were fed C until 4 wk of age and then were assigned to 1 of 6 weight-matched groups that were fed C, HE, C+HE, C+EN, HE+EN, or C+HE+EN for 5 wk. EN accelerated weight gain and increased energy intake and adiposity irrespective of the solid diet consumed. Serum leptin concentrations were increased after the consumption of all diets when compared with C rats, but there was dissociation between leptin levels and adiposity. The type of solid diet had no effect on the expression of a panel of hypothalamic genes except for glutamate-decarboxylase-67. EN decreased mRNA for agouti-related peptide and neuropeptide Y in the arcuate nucleus and DYN in the paraventricular nucleus. Dynorphin and CART mRNA were decreased in the supraoptic retrochiasmatic nucleus. The reduction in orexigenic signaling in the hypothalamus suggests that overconsumption of EN is sensed by the hypothalamus but that any initiated physiological responses fail to compensate effectively and may be negated or overwhelmed by other systems. Providing diets in solid and liquid form, with choice, mimics more closely the human environment. Understanding the interactions between these diets and peripheral and central energy balance systems could be crucial in unraveling the events underlying human obesity and its early development.

AB - Contemporary foods and beverages that constitute the diets of adults and children almost certainly contribute to the obesity problem. To develop a model of childhood obesity, we examined the effects of feeding juvenile rats 2 solid diets, either alone or in combination [nonpurified control diet (C), high-energy (HE), or C+HE] with or without the liquid supplement Ensure (EN). Rats were fed C until 4 wk of age and then were assigned to 1 of 6 weight-matched groups that were fed C, HE, C+HE, C+EN, HE+EN, or C+HE+EN for 5 wk. EN accelerated weight gain and increased energy intake and adiposity irrespective of the solid diet consumed. Serum leptin concentrations were increased after the consumption of all diets when compared with C rats, but there was dissociation between leptin levels and adiposity. The type of solid diet had no effect on the expression of a panel of hypothalamic genes except for glutamate-decarboxylase-67. EN decreased mRNA for agouti-related peptide and neuropeptide Y in the arcuate nucleus and DYN in the paraventricular nucleus. Dynorphin and CART mRNA were decreased in the supraoptic retrochiasmatic nucleus. The reduction in orexigenic signaling in the hypothalamus suggests that overconsumption of EN is sensed by the hypothalamus but that any initiated physiological responses fail to compensate effectively and may be negated or overwhelmed by other systems. Providing diets in solid and liquid form, with choice, mimics more closely the human environment. Understanding the interactions between these diets and peripheral and central energy balance systems could be crucial in unraveling the events underlying human obesity and its early development.

KW - high-energy diet

KW - body-weight

KW - messenger-RNA

KW - food-intake

KW - neronal activation

KW - induced hyperphagia

KW - childhood obesity

KW - resistant rats

KW - leptin

KW - balance

M3 - Article

VL - 137

SP - 1483

EP - 1490

JO - The Journal of Nutrition

JF - The Journal of Nutrition

SN - 0022-3166

IS - 6

ER -