Soluble Dectin-1 as a tool to detect beta-glucans

Lisa M. Graham, S. Vicky Tsoni, Janet Anne Willment, David L. Williams, Philip R. Taylor, Siamon Gordon, Kevin M Dennehy, Gordon Douglas Brown

Research output: Contribution to journalArticlepeer-review

92 Citations (Scopus)


Beta-glucans are structural components of fungal cell walls which are involved in the immune recognition of fungal pathogens and possess beneficial immunomodulatory activities in isolated form. Here we have developed a soluble chimeric form of the major mammalian beta-glucan receptor, Dectin-1, and demonstrate its application for the detection and characterisation of soluble and insoluble beta-glucans, including fungal particles, using ELISA, flow cytometric and fluorescence-based microscopy assays.
Original languageEnglish
Pages (from-to)164-169
Number of pages6
JournalJournal of Immunological Methods
Issue number1-2
Early online date23 Jun 2006
Publication statusPublished - Jul 2006


  • Antibody Specificity
  • Cell Line
  • Cell Wall
  • Enzyme-Linked Immunosorbent Assay
  • Flow Cytometry
  • Fungal Proteins
  • Glucans
  • Humans
  • Membrane Proteins
  • Microscopy, Fluorescence
  • Nerve Tissue Proteins
  • Recombinant Fusion Proteins
  • Saccharomyces cerevisiae
  • Solubility
  • beta-Glucans


Dive into the research topics of 'Soluble Dectin-1 as a tool to detect beta-glucans'. Together they form a unique fingerprint.

Cite this