Soluble Dectin-1 as a tool to detect beta-glucans

Lisa M.  Graham; S. Vicky Tsoni; Janet Anne Willment; David L. Williams; Philip R. Taylor; Siamon Gordon; Kevin M Dennehy; Gordon Douglas Brown

doi:10.1016/j.jim.2006.05.013

Soluble Dectin-1 as a tool to detect beta-glucans

Lisa M. Graham, S. Vicky Tsoni, Janet Anne Willment, David L. Williams, Philip R. Taylor, Siamon Gordon, Kevin M Dennehy, Gordon Douglas Brown

Applied Medicine

Research output: Contribution to journal › Article › peer-review

99 Citations (Scopus)

Abstract

Beta-glucans are structural components of fungal cell walls which are involved in the immune recognition of fungal pathogens and possess beneficial immunomodulatory activities in isolated form. Here we have developed a soluble chimeric form of the major mammalian beta-glucan receptor, Dectin-1, and demonstrate its application for the detection and characterisation of soluble and insoluble beta-glucans, including fungal particles, using ELISA, flow cytometric and fluorescence-based microscopy assays.

Original language	English
Pages (from-to)	164-169
Number of pages	6
Journal	Journal of Immunological Methods
Volume	314
Issue number	1-2
Early online date	23 Jun 2006
DOIs	https://doi.org/10.1016/j.jim.2006.05.013
Publication status	Published - Jul 2006

Keywords

Antibody Specificity
Cell Line
Cell Wall
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Fungal Proteins
Glucans
Humans
Membrane Proteins
Microscopy, Fluorescence
Nerve Tissue Proteins
Recombinant Fusion Proteins
Saccharomyces cerevisiae
Solubility
beta-Glucans

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title = "Soluble Dectin-1 as a tool to detect beta-glucans",

abstract = "Beta-glucans are structural components of fungal cell walls which are involved in the immune recognition of fungal pathogens and possess beneficial immunomodulatory activities in isolated form. Here we have developed a soluble chimeric form of the major mammalian beta-glucan receptor, Dectin-1, and demonstrate its application for the detection and characterisation of soluble and insoluble beta-glucans, including fungal particles, using ELISA, flow cytometric and fluorescence-based microscopy assays.",

keywords = "Antibody Specificity, Cell Line, Cell Wall, Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Fungal Proteins, Glucans, Humans, Membrane Proteins, Microscopy, Fluorescence, Nerve Tissue Proteins, Recombinant Fusion Proteins, Saccharomyces cerevisiae, Solubility, beta-Glucans",

author = "Graham, {Lisa M.} and Tsoni, {S. Vicky} and Willment, {Janet Anne} and Williams, {David L.} and Taylor, {Philip R.} and Siamon Gordon and Dennehy, {Kevin M} and Brown, {Gordon Douglas}",

year = "2006",

month = jul,

doi = "10.1016/j.jim.2006.05.013",

language = "English",

volume = "314",

pages = "164--169",

journal = "Journal of Immunological Methods",

issn = "0022-1759",

publisher = "Elsevier",

number = "1-2",

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TY - JOUR

T1 - Soluble Dectin-1 as a tool to detect beta-glucans

AU - Graham, Lisa M.

AU - Tsoni, S. Vicky

AU - Willment, Janet Anne

AU - Williams, David L.

AU - Taylor, Philip R.

AU - Gordon, Siamon

AU - Dennehy, Kevin M

AU - Brown, Gordon Douglas

PY - 2006/7

Y1 - 2006/7

N2 - Beta-glucans are structural components of fungal cell walls which are involved in the immune recognition of fungal pathogens and possess beneficial immunomodulatory activities in isolated form. Here we have developed a soluble chimeric form of the major mammalian beta-glucan receptor, Dectin-1, and demonstrate its application for the detection and characterisation of soluble and insoluble beta-glucans, including fungal particles, using ELISA, flow cytometric and fluorescence-based microscopy assays.

AB - Beta-glucans are structural components of fungal cell walls which are involved in the immune recognition of fungal pathogens and possess beneficial immunomodulatory activities in isolated form. Here we have developed a soluble chimeric form of the major mammalian beta-glucan receptor, Dectin-1, and demonstrate its application for the detection and characterisation of soluble and insoluble beta-glucans, including fungal particles, using ELISA, flow cytometric and fluorescence-based microscopy assays.

KW - Antibody Specificity

KW - Cell Line

KW - Cell Wall

KW - Enzyme-Linked Immunosorbent Assay

KW - Flow Cytometry

KW - Fungal Proteins

KW - Glucans

KW - Humans

KW - Membrane Proteins

KW - Microscopy, Fluorescence

KW - Nerve Tissue Proteins

KW - Recombinant Fusion Proteins

KW - Saccharomyces cerevisiae

KW - Solubility

KW - beta-Glucans

U2 - 10.1016/j.jim.2006.05.013

DO - 10.1016/j.jim.2006.05.013

M3 - Article

C2 - 16844139

SN - 0022-1759

VL - 314

SP - 164

EP - 169

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

IS - 1-2

ER -

Soluble Dectin-1 as a tool to detect beta-glucans

Abstract

Keywords

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