Soluble Dectin-1 as a tool to detect beta-glucans

Lisa M. Graham, S. Vicky Tsoni, Janet Anne Willment, David L. Williams, Philip R. Taylor, Siamon Gordon, Kevin M Dennehy, Gordon Douglas Brown

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Abstract

Beta-glucans are structural components of fungal cell walls which are involved in the immune recognition of fungal pathogens and possess beneficial immunomodulatory activities in isolated form. Here we have developed a soluble chimeric form of the major mammalian beta-glucan receptor, Dectin-1, and demonstrate its application for the detection and characterisation of soluble and insoluble beta-glucans, including fungal particles, using ELISA, flow cytometric and fluorescence-based microscopy assays.
Original languageEnglish
Pages (from-to)164-169
Number of pages6
JournalJournal of Immunological Methods
Volume314
Issue number1-2
Early online date23 Jun 2006
DOIs
Publication statusPublished - Jul 2006

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Keywords

  • Antibody Specificity
  • Cell Line
  • Cell Wall
  • Enzyme-Linked Immunosorbent Assay
  • Flow Cytometry
  • Fungal Proteins
  • Glucans
  • Humans
  • Membrane Proteins
  • Microscopy, Fluorescence
  • Nerve Tissue Proteins
  • Recombinant Fusion Proteins
  • Saccharomyces cerevisiae
  • Solubility
  • beta-Glucans

Cite this

Graham, L. M., Tsoni, S. V., Willment, J. A., Williams, D. L., Taylor, P. R., Gordon, S., Dennehy, K. M., & Brown, G. D. (2006). Soluble Dectin-1 as a tool to detect beta-glucans. Journal of Immunological Methods, 314(1-2), 164-169. https://doi.org/10.1016/j.jim.2006.05.013