Stimulation of actin and myosin synthesis in rat gastrocnemius muscle by clenbuterol: Evidence for translational control

J E HESKETH, Gillian Patricia Campbell, Gerald Lobley, Charlotte Maltin, F ACAMOVIC, R M PALMER

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Abstract

1. A transient rise in fractional rates of protein and actomyosin synthesis was observed in gastrocneumius muscles of rats fed clenbuterol for 1-2 days but the muscle RNA:protein ratio was unchanged, therefore protein synthesis per unit RNA (k(RNA)) also increased.

2. Myosin heavy and light chains and actin showed increased incorporation of [H-3]phenylalanine at 2 days; these changes were proportional to increases in total protein synthesis.

3. The ratios actin mRNA : 18S RNA and fast myosin heavy chain mRNA : 18S RNA were unaffected by clenbuterol.

4. The data suggest that the clenbuterol-induced increase in muscle protein synthesis involves both translational control and increased tissue RNA.

Original languageEnglish
Pages (from-to)23-27
Number of pages5
JournalComparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
Volume102
Issue number1
DOIs
Publication statusPublished - May 1992

Keywords

  • protein-synthesis
  • skeletal-muscle
  • messenger-RNA
  • agonist clenbuterol
  • metabolism
  • ractopamine
  • growth
  • degradation
  • hypertrophy
  • ibvivo

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