Stimulation of actin and myosin synthesis in rat gastrocnemius muscle by clenbuterol: Evidence for translational control

J E  HESKETH; Gillian Patricia Campbell; Gerald Lobley; Charlotte Maltin; F  ACAMOVIC; R M  PALMER

doi:10.1016/0742-8413(92)90037-8

Stimulation of actin and myosin synthesis in rat gastrocnemius muscle by clenbuterol: Evidence for translational control

J E HESKETH, Gillian Patricia Campbell, Gerald Lobley, Charlotte Maltin, F ACAMOVIC, R M PALMER

The Rowett Institute of Nutrition and Health

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Abstract

1. A transient rise in fractional rates of protein and actomyosin synthesis was observed in gastrocneumius muscles of rats fed clenbuterol for 1-2 days but the muscle RNA:protein ratio was unchanged, therefore protein synthesis per unit RNA (k(RNA)) also increased.

2. Myosin heavy and light chains and actin showed increased incorporation of [H-3]phenylalanine at 2 days; these changes were proportional to increases in total protein synthesis.

3. The ratios actin mRNA : 18S RNA and fast myosin heavy chain mRNA : 18S RNA were unaffected by clenbuterol.

4. The data suggest that the clenbuterol-induced increase in muscle protein synthesis involves both translational control and increased tissue RNA.

Original language	English
Pages (from-to)	23-27
Number of pages	5
Journal	Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
Volume	102
Issue number	1
DOIs	https://doi.org/10.1016/0742-8413(92)90037-8
Publication status	Published - May 1992

Keywords

protein-synthesis
skeletal-muscle
messenger-RNA
agonist clenbuterol
metabolism
ractopamine
growth
degradation
hypertrophy
ibvivo

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Stimulation of actin and myosin synthesis in rat gastrocnemius muscle by clenbuterol: Evidence for translational control . / HESKETH, J E ; Campbell, Gillian Patricia; Lobley, Gerald et al.
In: Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, Vol. 102, No. 1, 05.1992, p. 23-27.

Research output: Contribution to journal › Article › peer-review

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KW - ractopamine

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KW - degradation

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Stimulation of actin and myosin synthesis in rat gastrocnemius muscle by clenbuterol: Evidence for translational control

Abstract

Keywords

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