Structures of two major histocompatibility complex class I genes of the rainbow trout (Oncorrhynchus mykiss).

B. P. Shum, P. M. Mason, K. E. Magor, L. R. Flodin, Rene Josephus Maria Stet, P. Parham

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    19 Citations (Scopus)

    Abstract

    Here we describe two rainbow trout major histocompatibility complex (MHC) class I genes characterized from lambda phage genomic clones prepared from a single fish. Clone GC71 contains all exons except a leader peptide-encoding exon. An open reading frame is maintained, and thus the gene MhcOnmy-U71 could be expressed in this individual. The class I gene found on clone GC41 lacks exons encoding the leader peptide and cytoplasmic domain. This gene, MhcOnmy-U41p, is a pseudogene due to a deletion in the alpha(2) domain-encoding exon causing premature termination. Both the Onmy-U71 and Onmy-U41p genes are distinguished by long introns between the exons encoding the alpha(1) and alpha(2) domains. Clone GC41 also contains the 3' exons of the LMP71 PSMB8 gene encoding the gamma-interferon-induced proteosome subunit of rainbow trout.

    Original languageEnglish
    Pages (from-to)193-199
    Number of pages6
    JournalImmunogenetics
    Volume54
    DOIs
    Publication statusPublished - 2002

    Keywords

    • comparative immunology
    • evolution
    • MHC class I genes
    • LMP7
    • rainbow trout
    • MHC CLASS-I
    • PUFFERFISH FUGU RUBRIPES
    • ZEBRAFISH
    • LINKAGE
    • IDENTIFICATION
    • EXPRESSION
    • DIVERSITY
    • REGION
    • RERIO

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