Subtle population structuring within a highly vagile marine invertebrate, the veined squid Loligo forbesi, demonstrated with microsatellite DNA markers

P W Shaw, G J Pierce, P R Boyle

Research output: Contribution to journalArticle

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Abstract

Microsatellite DNA markers were applied for the first time in a population genetic study of a cephalopod and compared with previous estimates of genetic differentiation obtained using allozyme and mitochondrial DNA (mtDNA) markers. Levels of genetic variation detected with microsatellites were much higher than found with previous markers (mean number of alleles per locus = 10.6, mean expected heterozygosity (H-E) = 0.79; allozyme H-E = 0.08; mtDNA restriction fragment length polymorphism (RFLP) H-E = 0.16). In agreement with previous studies, microsatellites demonstrated genetic uniformity across the population occupying the European shelf seas of the North East Atlantic, and extreme genetic differentiation of the Azores population (R-ST/F-ST = 0.252/0.245; allozyme F-ST = 0.536; mtDNA F-ST = 0.789). In contrast to other markers, microsatellites detected more subtle, and significant, levels of differentiation between the populations of the North East Atlantic offshore banks (Rockall and Faroes) and the shelf population (R-ST = 0.048 and 0.057). Breakdown of extensive gene flow among these populations is indicated, with hydrographic (water depth) and hydrodynamic (isolating current regimes) factors suggested as possible barriers to migration. The demonstration of genetic subdivision in an abundant, highly mobile marine invertebrate has implications for the interpretation of dispersal and population dynamics, and consequent management, of such a commercially exploited species. Relative levels of differentiation indicated by the three different marker systems, and the use of measures of differentiation (assuming different mutation models), are discussed.

Original languageEnglish
Pages (from-to)407-417
Number of pages11
JournalMolecular Ecology
Volume8
Publication statusPublished - 1999

Keywords

  • Cephalopoda
  • Loligo
  • microsatellite DNA
  • population genetics
  • squid
  • stock structure
  • COD GADUS-MORHUA
  • NORTHEAST ATLANTIC-OCEAN
  • GENETIC DIFFERENTIATION
  • NATURAL-POPULATIONS
  • NORTHWEST ATLANTIC
  • ALLELE FREQUENCIES
  • MUTATION
  • POLYMORPHISM
  • STOCK
  • LOCI

Cite this

Subtle population structuring within a highly vagile marine invertebrate, the veined squid Loligo forbesi, demonstrated with microsatellite DNA markers. / Shaw, P W ; Pierce, G J ; Boyle, P R .

In: Molecular Ecology, Vol. 8, 1999, p. 407-417.

Research output: Contribution to journalArticle

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AU - Pierce, G J

AU - Boyle, P R

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N2 - Microsatellite DNA markers were applied for the first time in a population genetic study of a cephalopod and compared with previous estimates of genetic differentiation obtained using allozyme and mitochondrial DNA (mtDNA) markers. Levels of genetic variation detected with microsatellites were much higher than found with previous markers (mean number of alleles per locus = 10.6, mean expected heterozygosity (H-E) = 0.79; allozyme H-E = 0.08; mtDNA restriction fragment length polymorphism (RFLP) H-E = 0.16). In agreement with previous studies, microsatellites demonstrated genetic uniformity across the population occupying the European shelf seas of the North East Atlantic, and extreme genetic differentiation of the Azores population (R-ST/F-ST = 0.252/0.245; allozyme F-ST = 0.536; mtDNA F-ST = 0.789). In contrast to other markers, microsatellites detected more subtle, and significant, levels of differentiation between the populations of the North East Atlantic offshore banks (Rockall and Faroes) and the shelf population (R-ST = 0.048 and 0.057). Breakdown of extensive gene flow among these populations is indicated, with hydrographic (water depth) and hydrodynamic (isolating current regimes) factors suggested as possible barriers to migration. The demonstration of genetic subdivision in an abundant, highly mobile marine invertebrate has implications for the interpretation of dispersal and population dynamics, and consequent management, of such a commercially exploited species. Relative levels of differentiation indicated by the three different marker systems, and the use of measures of differentiation (assuming different mutation models), are discussed.

AB - Microsatellite DNA markers were applied for the first time in a population genetic study of a cephalopod and compared with previous estimates of genetic differentiation obtained using allozyme and mitochondrial DNA (mtDNA) markers. Levels of genetic variation detected with microsatellites were much higher than found with previous markers (mean number of alleles per locus = 10.6, mean expected heterozygosity (H-E) = 0.79; allozyme H-E = 0.08; mtDNA restriction fragment length polymorphism (RFLP) H-E = 0.16). In agreement with previous studies, microsatellites demonstrated genetic uniformity across the population occupying the European shelf seas of the North East Atlantic, and extreme genetic differentiation of the Azores population (R-ST/F-ST = 0.252/0.245; allozyme F-ST = 0.536; mtDNA F-ST = 0.789). In contrast to other markers, microsatellites detected more subtle, and significant, levels of differentiation between the populations of the North East Atlantic offshore banks (Rockall and Faroes) and the shelf population (R-ST = 0.048 and 0.057). Breakdown of extensive gene flow among these populations is indicated, with hydrographic (water depth) and hydrodynamic (isolating current regimes) factors suggested as possible barriers to migration. The demonstration of genetic subdivision in an abundant, highly mobile marine invertebrate has implications for the interpretation of dispersal and population dynamics, and consequent management, of such a commercially exploited species. Relative levels of differentiation indicated by the three different marker systems, and the use of measures of differentiation (assuming different mutation models), are discussed.

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KW - Loligo

KW - microsatellite DNA

KW - population genetics

KW - squid

KW - stock structure

KW - COD GADUS-MORHUA

KW - NORTHEAST ATLANTIC-OCEAN

KW - GENETIC DIFFERENTIATION

KW - NATURAL-POPULATIONS

KW - NORTHWEST ATLANTIC

KW - ALLELE FREQUENCIES

KW - MUTATION

KW - POLYMORPHISM

KW - STOCK

KW - LOCI

M3 - Article

VL - 8

SP - 407

EP - 417

JO - Molecular Ecology

JF - Molecular Ecology

SN - 0962-1083

ER -