Systemic CD4(+) T cell phenotype and activation status in intermediate uveitis

C. C. Murphy, Linda Duncan, John Vincent Forrester, A. D. Dick

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33 Citations (Scopus)

Abstract

Aim: To investigate peripheral blood lymphocyte phenotype in patients with intermediate uveitis using CD69, chemokine receptor, and cytokine expression.

Methods: Peripheral blood lymphocytes of 18 patients with idiopathic intermediate uveitis and 6 patients with presumed sarcoid intermediate uveitis were evaluated for CD4(+) T cell expression of CD69, CCR4, CCR5, CXCR3 and the intracellular cytokines IFNgamma, TNFalpha, and interleukin ( IL)-10 by flow cytometry, and for IL-2, IL-4, IL-5, IL-10, IFNgamma, and TNFalpha production following unstimulated and activated culture using cytokine bead array and compared with healthy control subjects.

Results: The expression of CD69 and TNFa by peripheral blood CD4+ lymphocytes of patients with idiopathic intermediate uveitis and presumed sarcoid intermediate uveitis was significantly higher than control subjects ( p = 0.002 and p< 0.05, respectively). The ratios of the concentrations of IL-2: IL-5 and IFN&gamma;: IL-5 in supernatants of activated peripheral blood lymphocyte cultures were significantly higher in patients with presumed sarcoid intermediate uveitis than control subjects.

Conclusions: This study implicates TNFa in the pathogenesis of intermediate uveitis, highlighting the potential role of anti-TNF treatments for this disease. Studies of Th1: Th2 cytokine ratios suggested polarisation of the immune response towards Th1 in presumed sarcoid intermediate uveitis despite clinically quiescent systemic disease.

Original languageEnglish
Pages (from-to)412-416
Number of pages4
JournalBritish Journal of Ophthalmology
Volume88
Issue number3
DOIs
Publication statusPublished - 2004

Keywords

  • EXPERIMENTAL AUTOIMMUNE UVEORETINITIS
  • NECROSIS-FACTOR-ALPHA
  • INTERFERON-GAMMA
  • DISEASE-ACTIVITY
  • BEHCETS-DISEASE
  • AQUEOUS-HUMOR
  • SERUM-LEVELS
  • LYMPHOCYTES
  • EXPRESSION
  • CYTOKINES

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