Temporal repression of core circadian genes is mediated through EARLY FLOWERING 3 in Arabidopsis

Laura E. Dixon, Kirsten Knox, Laszlo Kozma-Bognar, Megan M. Southern, Alexandra Pokhilko, Andrew J. Millar

Research output: Contribution to journalArticle

136 Citations (Scopus)

Abstract

The circadian clock provides robust, similar to 24 hr biological rhythms throughout the eukaryotes. The clock gene circuit in plants comprises interlocking transcriptional feedback loops, reviewed in [1], whereby the morning-expressed transcription factors CIRCADIAN CLOCK-ASSOCIATED 1 (CCA1) and LATE ELONGATED HYPOCOTYL (LHY) repress the expression of evening genes, notably TIMING OF CAB EXPRESSION 1 (TOC1). EARLY FLOWERING 3 (ELF3) has been implicated as a repressor of light signaling to the clock [2, 3] and, paradoxically, as an activator of the light-induced genes CCA1 and LHY [4, 5]. We use cca1-11 lhy-21 elf3-4 plants to separate the repressive function of ELF3 from its downstream targets CCA1 and LHY. We further demonstrate that ELF3 associates physically with the promoter of PSEUDO-RESPONSE REGULATOR 9 (PRR9), a repressor of CCA1 and LHY expression, in a time-dependent fashion. The repressive function of ELF3 is thus consistent with indirect activation of LHY and CCA1, in a double-negative connection via a direct ELF3 target, PRR9. This mechanism reconciles the functions of ELF3 in the clock network during the night and points to further effects of ELF3 during the day.

Original languageEnglish
Pages (from-to)120-125
Number of pages6
JournalCurrent Biology
Volume21
Issue number2
DOIs
Publication statusPublished - 25 Jan 2011

Keywords

  • photoperiodic control
  • distinct roles
  • clock
  • protein
  • ELF3
  • time
  • thaliana
  • gigantea
  • encodes
  • rhythms

Cite this

Dixon, L. E., Knox, K., Kozma-Bognar, L., Southern, M. M., Pokhilko, A., & Millar, A. J. (2011). Temporal repression of core circadian genes is mediated through EARLY FLOWERING 3 in Arabidopsis. Current Biology, 21(2), 120-125. https://doi.org/10.1016/j.cub.2010.12.013

Temporal repression of core circadian genes is mediated through EARLY FLOWERING 3 in Arabidopsis. / Dixon, Laura E.; Knox, Kirsten; Kozma-Bognar, Laszlo; Southern, Megan M.; Pokhilko, Alexandra; Millar, Andrew J.

In: Current Biology, Vol. 21, No. 2, 25.01.2011, p. 120-125.

Research output: Contribution to journalArticle

Dixon, LE, Knox, K, Kozma-Bognar, L, Southern, MM, Pokhilko, A & Millar, AJ 2011, 'Temporal repression of core circadian genes is mediated through EARLY FLOWERING 3 in Arabidopsis', Current Biology, vol. 21, no. 2, pp. 120-125. https://doi.org/10.1016/j.cub.2010.12.013
Dixon, Laura E. ; Knox, Kirsten ; Kozma-Bognar, Laszlo ; Southern, Megan M. ; Pokhilko, Alexandra ; Millar, Andrew J. / Temporal repression of core circadian genes is mediated through EARLY FLOWERING 3 in Arabidopsis. In: Current Biology. 2011 ; Vol. 21, No. 2. pp. 120-125.
@article{de5230bea5784616ad9bb80c3437e298,
title = "Temporal repression of core circadian genes is mediated through EARLY FLOWERING 3 in Arabidopsis",
abstract = "The circadian clock provides robust, similar to 24 hr biological rhythms throughout the eukaryotes. The clock gene circuit in plants comprises interlocking transcriptional feedback loops, reviewed in [1], whereby the morning-expressed transcription factors CIRCADIAN CLOCK-ASSOCIATED 1 (CCA1) and LATE ELONGATED HYPOCOTYL (LHY) repress the expression of evening genes, notably TIMING OF CAB EXPRESSION 1 (TOC1). EARLY FLOWERING 3 (ELF3) has been implicated as a repressor of light signaling to the clock [2, 3] and, paradoxically, as an activator of the light-induced genes CCA1 and LHY [4, 5]. We use cca1-11 lhy-21 elf3-4 plants to separate the repressive function of ELF3 from its downstream targets CCA1 and LHY. We further demonstrate that ELF3 associates physically with the promoter of PSEUDO-RESPONSE REGULATOR 9 (PRR9), a repressor of CCA1 and LHY expression, in a time-dependent fashion. The repressive function of ELF3 is thus consistent with indirect activation of LHY and CCA1, in a double-negative connection via a direct ELF3 target, PRR9. This mechanism reconciles the functions of ELF3 in the clock network during the night and points to further effects of ELF3 during the day.",
keywords = "photoperiodic control, distinct roles, clock, protein, ELF3, time, thaliana, gigantea, encodes, rhythms",
author = "Dixon, {Laura E.} and Kirsten Knox and Laszlo Kozma-Bognar and Southern, {Megan M.} and Alexandra Pokhilko and Millar, {Andrew J.}",
year = "2011",
month = "1",
day = "25",
doi = "10.1016/j.cub.2010.12.013",
language = "English",
volume = "21",
pages = "120--125",
journal = "Current Biology",
issn = "0960-9822",
publisher = "Cell Press",
number = "2",

}

TY - JOUR

T1 - Temporal repression of core circadian genes is mediated through EARLY FLOWERING 3 in Arabidopsis

AU - Dixon, Laura E.

AU - Knox, Kirsten

AU - Kozma-Bognar, Laszlo

AU - Southern, Megan M.

AU - Pokhilko, Alexandra

AU - Millar, Andrew J.

PY - 2011/1/25

Y1 - 2011/1/25

N2 - The circadian clock provides robust, similar to 24 hr biological rhythms throughout the eukaryotes. The clock gene circuit in plants comprises interlocking transcriptional feedback loops, reviewed in [1], whereby the morning-expressed transcription factors CIRCADIAN CLOCK-ASSOCIATED 1 (CCA1) and LATE ELONGATED HYPOCOTYL (LHY) repress the expression of evening genes, notably TIMING OF CAB EXPRESSION 1 (TOC1). EARLY FLOWERING 3 (ELF3) has been implicated as a repressor of light signaling to the clock [2, 3] and, paradoxically, as an activator of the light-induced genes CCA1 and LHY [4, 5]. We use cca1-11 lhy-21 elf3-4 plants to separate the repressive function of ELF3 from its downstream targets CCA1 and LHY. We further demonstrate that ELF3 associates physically with the promoter of PSEUDO-RESPONSE REGULATOR 9 (PRR9), a repressor of CCA1 and LHY expression, in a time-dependent fashion. The repressive function of ELF3 is thus consistent with indirect activation of LHY and CCA1, in a double-negative connection via a direct ELF3 target, PRR9. This mechanism reconciles the functions of ELF3 in the clock network during the night and points to further effects of ELF3 during the day.

AB - The circadian clock provides robust, similar to 24 hr biological rhythms throughout the eukaryotes. The clock gene circuit in plants comprises interlocking transcriptional feedback loops, reviewed in [1], whereby the morning-expressed transcription factors CIRCADIAN CLOCK-ASSOCIATED 1 (CCA1) and LATE ELONGATED HYPOCOTYL (LHY) repress the expression of evening genes, notably TIMING OF CAB EXPRESSION 1 (TOC1). EARLY FLOWERING 3 (ELF3) has been implicated as a repressor of light signaling to the clock [2, 3] and, paradoxically, as an activator of the light-induced genes CCA1 and LHY [4, 5]. We use cca1-11 lhy-21 elf3-4 plants to separate the repressive function of ELF3 from its downstream targets CCA1 and LHY. We further demonstrate that ELF3 associates physically with the promoter of PSEUDO-RESPONSE REGULATOR 9 (PRR9), a repressor of CCA1 and LHY expression, in a time-dependent fashion. The repressive function of ELF3 is thus consistent with indirect activation of LHY and CCA1, in a double-negative connection via a direct ELF3 target, PRR9. This mechanism reconciles the functions of ELF3 in the clock network during the night and points to further effects of ELF3 during the day.

KW - photoperiodic control

KW - distinct roles

KW - clock

KW - protein

KW - ELF3

KW - time

KW - thaliana

KW - gigantea

KW - encodes

KW - rhythms

U2 - 10.1016/j.cub.2010.12.013

DO - 10.1016/j.cub.2010.12.013

M3 - Article

VL - 21

SP - 120

EP - 125

JO - Current Biology

JF - Current Biology

SN - 0960-9822

IS - 2

ER -