The activity of the high-affinity K+ uptake system Kdp sensitizes cells of Escherichia coli to methylglyoxal

Gail Patricia Ferguson; A D Chacko; C H Lee; I R Booth

The activity of the high-affinity K+ uptake system Kdp sensitizes cells of Escherichia coli to methylglyoxal

Gail Patricia Ferguson, A D Chacko, C H Lee, I R Booth

Research output: Contribution to journal › Article › peer-review

Abstract

Expression of the Kdp system sensitizes cells to methylglyoxal (MG) whether this electrophile is added externally or is synthesized endogenously. The basis of this enhanced sensitivity is the maintenance of a higher cytoplasmic pH (pHi) in cells expressing Kdp. In such cells, MG elicits rapid cytoplasmic acidification via KefB and KefC, but the steady-state pHi attained is still too high to confer protection Lowering pHi further by incubation with acetate increases the sensitivity of cells to MG.

Original language	English
Pages (from-to)	3957-61
Number of pages	5
Journal	Journal of Bacteriology
Volume	178
Issue number	13
Publication status	Published - 1 Jul 1996

Keywords

Adenosine Triphosphatases
Antiporters
Bacterial Proteins
Carrier Proteins
Cation Transport Proteins
Escherichia coli
Escherichia coli Proteins
Hydrogen-Ion Concentration
Potassium
Potassium Channels
Potassium-Hydrogen Antiporters
Pyruvaldehyde

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title = "The activity of the high-affinity K+ uptake system Kdp sensitizes cells of Escherichia coli to methylglyoxal",

abstract = "Expression of the Kdp system sensitizes cells to methylglyoxal (MG) whether this electrophile is added externally or is synthesized endogenously. The basis of this enhanced sensitivity is the maintenance of a higher cytoplasmic pH (pHi) in cells expressing Kdp. In such cells, MG elicits rapid cytoplasmic acidification via KefB and KefC, but the steady-state pHi attained is still too high to confer protection Lowering pHi further by incubation with acetate increases the sensitivity of cells to MG.",

keywords = "Adenosine Triphosphatases, Antiporters, Bacterial Proteins, Carrier Proteins, Cation Transport Proteins, Escherichia coli, Escherichia coli Proteins, Hydrogen-Ion Concentration, Potassium, Potassium Channels, Potassium-Hydrogen Antiporters, Pyruvaldehyde",

author = "Ferguson, {Gail Patricia} and Chacko, {A D} and Lee, {C H} and Booth, {I R}",

year = "1996",

month = jul,

day = "1",

language = "English",

volume = "178",

pages = "3957--61",

journal = "Journal of Bacteriology",

issn = "0021-9193",

publisher = "American Society for Microbiology",

number = "13",

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TY - JOUR

T1 - The activity of the high-affinity K+ uptake system Kdp sensitizes cells of Escherichia coli to methylglyoxal

AU - Ferguson, Gail Patricia

AU - Chacko, A D

AU - Lee, C H

AU - Booth, I R

PY - 1996/7/1

Y1 - 1996/7/1

N2 - Expression of the Kdp system sensitizes cells to methylglyoxal (MG) whether this electrophile is added externally or is synthesized endogenously. The basis of this enhanced sensitivity is the maintenance of a higher cytoplasmic pH (pHi) in cells expressing Kdp. In such cells, MG elicits rapid cytoplasmic acidification via KefB and KefC, but the steady-state pHi attained is still too high to confer protection Lowering pHi further by incubation with acetate increases the sensitivity of cells to MG.

AB - Expression of the Kdp system sensitizes cells to methylglyoxal (MG) whether this electrophile is added externally or is synthesized endogenously. The basis of this enhanced sensitivity is the maintenance of a higher cytoplasmic pH (pHi) in cells expressing Kdp. In such cells, MG elicits rapid cytoplasmic acidification via KefB and KefC, but the steady-state pHi attained is still too high to confer protection Lowering pHi further by incubation with acetate increases the sensitivity of cells to MG.

KW - Adenosine Triphosphatases

KW - Antiporters

KW - Bacterial Proteins

KW - Carrier Proteins

KW - Cation Transport Proteins

KW - Escherichia coli

KW - Escherichia coli Proteins

KW - Hydrogen-Ion Concentration

KW - Potassium

KW - Potassium Channels

KW - Potassium-Hydrogen Antiporters

KW - Pyruvaldehyde

M3 - Article

C2 - 8682804

SN - 0021-9193

VL - 178

SP - 3957

EP - 3961

JO - Journal of Bacteriology

JF - Journal of Bacteriology

IS - 13

ER -

The activity of the high-affinity K+ uptake system Kdp sensitizes cells of Escherichia coli to methylglyoxal

Abstract

Keywords

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