The activity of the high-affinity K+ uptake system Kdp sensitizes cells of Escherichia coli to methylglyoxal

Gail Patricia Ferguson, A D Chacko, C H Lee, I R Booth

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Expression of the Kdp system sensitizes cells to methylglyoxal (MG) whether this electrophile is added externally or is synthesized endogenously. The basis of this enhanced sensitivity is the maintenance of a higher cytoplasmic pH (pHi) in cells expressing Kdp. In such cells, MG elicits rapid cytoplasmic acidification via KefB and KefC, but the steady-state pHi attained is still too high to confer protection Lowering pHi further by incubation with acetate increases the sensitivity of cells to MG.
Original languageEnglish
Pages (from-to)3957-61
Number of pages5
JournalJournal of Bacteriology
Volume178
Issue number13
Publication statusPublished - 1 Jul 1996

Keywords

  • Adenosine Triphosphatases
  • Antiporters
  • Bacterial Proteins
  • Carrier Proteins
  • Cation Transport Proteins
  • Escherichia coli
  • Escherichia coli Proteins
  • Hydrogen-Ion Concentration
  • Potassium
  • Potassium Channels
  • Potassium-Hydrogen Antiporters
  • Pyruvaldehyde

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