The activity of the high-affinity K+ uptake system Kdp sensitizes cells of Escherichia coli to methylglyoxal

Gail Patricia Ferguson, A D Chacko, C H Lee, I R Booth

Research output: Contribution to journalArticle

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Abstract

Expression of the Kdp system sensitizes cells to methylglyoxal (MG) whether this electrophile is added externally or is synthesized endogenously. The basis of this enhanced sensitivity is the maintenance of a higher cytoplasmic pH (pHi) in cells expressing Kdp. In such cells, MG elicits rapid cytoplasmic acidification via KefB and KefC, but the steady-state pHi attained is still too high to confer protection Lowering pHi further by incubation with acetate increases the sensitivity of cells to MG.
Original languageEnglish
Pages (from-to)3957-61
Number of pages5
JournalJournal of Bacteriology
Volume178
Issue number13
Publication statusPublished - 1 Jul 1996

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Pyruvaldehyde
Escherichia coli
Acetates

Keywords

  • Adenosine Triphosphatases
  • Antiporters
  • Bacterial Proteins
  • Carrier Proteins
  • Cation Transport Proteins
  • Escherichia coli
  • Escherichia coli Proteins
  • Hydrogen-Ion Concentration
  • Potassium
  • Potassium Channels
  • Potassium-Hydrogen Antiporters
  • Pyruvaldehyde

Cite this

Ferguson, G. P., Chacko, A. D., Lee, C. H., & Booth, I. R. (1996). The activity of the high-affinity K+ uptake system Kdp sensitizes cells of Escherichia coli to methylglyoxal. Journal of Bacteriology, 178(13), 3957-61.

The activity of the high-affinity K+ uptake system Kdp sensitizes cells of Escherichia coli to methylglyoxal. / Ferguson, Gail Patricia; Chacko, A D; Lee, C H; Booth, I R.

In: Journal of Bacteriology, Vol. 178, No. 13, 01.07.1996, p. 3957-61.

Research output: Contribution to journalArticle

Ferguson, GP, Chacko, AD, Lee, CH & Booth, IR 1996, 'The activity of the high-affinity K+ uptake system Kdp sensitizes cells of Escherichia coli to methylglyoxal', Journal of Bacteriology, vol. 178, no. 13, pp. 3957-61.
Ferguson, Gail Patricia ; Chacko, A D ; Lee, C H ; Booth, I R. / The activity of the high-affinity K+ uptake system Kdp sensitizes cells of Escherichia coli to methylglyoxal. In: Journal of Bacteriology. 1996 ; Vol. 178, No. 13. pp. 3957-61.
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