The development of a DNA based method aimed at identifying the fish species present in food products

Georgina Louise Hold, Valerie Joan Russell, S. E. Pryde, H. Rehbein, J. Quinterio, R. Vidal, M. Rey-Mendez, C. G. Sotelo, R. I. Perez-Martin, A. Santos, C. Rosa

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Analysis of restriction fragment length polymorphism (RFLP) profiles of a 464 bp amplicon obtained from the mitochondrial cytochrome b gene was used to differentiate between several different fish species. The method was tested by a collaborative study in which 12 European laboratories participated to ascertain whether the method was reproducible. Each laboratory was required to identify 10 unknown samples by comparison with RFLP profiles from authentic species. From a total of 120 tests performed, unknown samples were correctly identified in 96% of cases. Further work attempting to use the method to analyze mixed and processed fish samples was also performed. In all cases the species contained within mixed samples were correctly identified, indicating:the efficacy of the method for detecting fraudulent substitution of fish species in food products.

Original languageEnglish
Pages (from-to)1175-1179
Number of pages4
JournalJournal of Agricultural and Food Chemistry
Volume49
Issue number3
DOIs
Publication statusPublished - 2001

Keywords

  • species identification
  • PCR-RFLP
  • cytochrome b
  • interlaboratory study
  • capillary zone electrophoresis
  • fragment-length-polymorphism
  • trout onchorhynchus-mykiss
  • salmon salmo-salar
  • identification
  • differentiation
  • gene

Cite this

Hold, G. L., Russell, V. J., Pryde, S. E., Rehbein, H., Quinterio, J., Vidal, R., Rey-Mendez, M., Sotelo, C. G., Perez-Martin, R. I., Santos, A., & Rosa, C. (2001). The development of a DNA based method aimed at identifying the fish species present in food products. Journal of Agricultural and Food Chemistry, 49(3), 1175-1179. https://doi.org/10.1021/jf001149x