The development of a DNA based method aimed at identifying the fish species present in food products

Georgina Louise Hold; Valerie Joan Russell; S. E. Pryde; H. Rehbein; J. Quinterio; R. Vidal; M. Rey-Mendez; C. G. Sotelo; R. I. Perez-Martin; A. Santos; C. Rosa

doi:10.1021/jf001149x

The development of a DNA based method aimed at identifying the fish species present in food products

Georgina Louise Hold, Valerie Joan Russell, S. E. Pryde, H. Rehbein, J. Quinterio, R. Vidal, M. Rey-Mendez, C. G. Sotelo, R. I. Perez-Martin, A. Santos, C. Rosa

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59 Citations (Scopus)

Abstract

Analysis of restriction fragment length polymorphism (RFLP) profiles of a 464 bp amplicon obtained from the mitochondrial cytochrome b gene was used to differentiate between several different fish species. The method was tested by a collaborative study in which 12 European laboratories participated to ascertain whether the method was reproducible. Each laboratory was required to identify 10 unknown samples by comparison with RFLP profiles from authentic species. From a total of 120 tests performed, unknown samples were correctly identified in 96% of cases. Further work attempting to use the method to analyze mixed and processed fish samples was also performed. In all cases the species contained within mixed samples were correctly identified, indicating:the efficacy of the method for detecting fraudulent substitution of fish species in food products.

Original language	English
Pages (from-to)	1175-1179
Number of pages	4
Journal	Journal of Agricultural and Food Chemistry
Volume	49
Issue number	3
DOIs	https://doi.org/10.1021/jf001149x
Publication status	Published - 2001

Keywords

species identification
PCR-RFLP
cytochrome b
interlaboratory study
capillary zone electrophoresis
fragment-length-polymorphism
trout onchorhynchus-mykiss
salmon salmo-salar
identification
differentiation
gene

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10.1021/jf001149x

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title = "The development of a DNA based method aimed at identifying the fish species present in food products",

abstract = "Analysis of restriction fragment length polymorphism (RFLP) profiles of a 464 bp amplicon obtained from the mitochondrial cytochrome b gene was used to differentiate between several different fish species. The method was tested by a collaborative study in which 12 European laboratories participated to ascertain whether the method was reproducible. Each laboratory was required to identify 10 unknown samples by comparison with RFLP profiles from authentic species. From a total of 120 tests performed, unknown samples were correctly identified in 96% of cases. Further work attempting to use the method to analyze mixed and processed fish samples was also performed. In all cases the species contained within mixed samples were correctly identified, indicating:the efficacy of the method for detecting fraudulent substitution of fish species in food products.",

keywords = "species identification, PCR-RFLP, cytochrome b, interlaboratory study, capillary zone electrophoresis, fragment-length-polymorphism, trout onchorhynchus-mykiss, salmon salmo-salar, identification, differentiation, gene",

author = "Hold, {Georgina Louise} and Russell, {Valerie Joan} and Pryde, {S. E.} and H. Rehbein and J. Quinterio and R. Vidal and M. Rey-Mendez and Sotelo, {C. G.} and Perez-Martin, {R. I.} and A. Santos and C. Rosa",

year = "2001",

doi = "10.1021/jf001149x",

language = "English",

volume = "49",

pages = "1175--1179",

journal = "Journal of Agricultural and Food Chemistry",

issn = "0021-8561",

publisher = "American Chemical Society",

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T1 - The development of a DNA based method aimed at identifying the fish species present in food products

AU - Hold, Georgina Louise

AU - Russell, Valerie Joan

AU - Pryde, S. E.

AU - Rehbein, H.

AU - Quinterio, J.

AU - Vidal, R.

AU - Rey-Mendez, M.

AU - Sotelo, C. G.

AU - Perez-Martin, R. I.

AU - Santos, A.

AU - Rosa, C.

PY - 2001

Y1 - 2001

N2 - Analysis of restriction fragment length polymorphism (RFLP) profiles of a 464 bp amplicon obtained from the mitochondrial cytochrome b gene was used to differentiate between several different fish species. The method was tested by a collaborative study in which 12 European laboratories participated to ascertain whether the method was reproducible. Each laboratory was required to identify 10 unknown samples by comparison with RFLP profiles from authentic species. From a total of 120 tests performed, unknown samples were correctly identified in 96% of cases. Further work attempting to use the method to analyze mixed and processed fish samples was also performed. In all cases the species contained within mixed samples were correctly identified, indicating:the efficacy of the method for detecting fraudulent substitution of fish species in food products.

AB - Analysis of restriction fragment length polymorphism (RFLP) profiles of a 464 bp amplicon obtained from the mitochondrial cytochrome b gene was used to differentiate between several different fish species. The method was tested by a collaborative study in which 12 European laboratories participated to ascertain whether the method was reproducible. Each laboratory was required to identify 10 unknown samples by comparison with RFLP profiles from authentic species. From a total of 120 tests performed, unknown samples were correctly identified in 96% of cases. Further work attempting to use the method to analyze mixed and processed fish samples was also performed. In all cases the species contained within mixed samples were correctly identified, indicating:the efficacy of the method for detecting fraudulent substitution of fish species in food products.

KW - species identification

KW - PCR-RFLP

KW - cytochrome b

KW - interlaboratory study

KW - capillary zone electrophoresis

KW - fragment-length-polymorphism

KW - trout onchorhynchus-mykiss

KW - salmon salmo-salar

KW - identification

KW - differentiation

KW - gene

U2 - 10.1021/jf001149x

DO - 10.1021/jf001149x

M3 - Article

SN - 0021-8561

VL - 49

SP - 1175

EP - 1179

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

IS - 3

ER -

The development of a DNA based method aimed at identifying the fish species present in food products

Abstract

Keywords

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