The effect of acute and chronic administration of the beta-agonist, cimaterol, on protein synthesisin ovine skin and muscle

J E NASH, H J G ROCHA, Vivien Buchan, Alexander Graham Calder, Eric Milne, J F QUIRKE, Gerald Lobley

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The action of intravenous infusion of the beta-agonist cimaterol (2.5 mg/d) on whole-body N retention and protein synthesis in peripheral tissues was examined in growing sheep. Wool growth was determined from skin patch clippings and adjusted to total fibre production. Protein synthesis was measured, using sequential large dose injections of [1-C-13]valine, leucine and phenylalanine and then [ring-d(5)]-phenylalanine, on biopsy samples from skin and m. longissimus dorsi taken before beta-agonist administration, at day 3 and day 15 of cimaterol infusion, and 15 d after withdrawal of the drug. Cimaterol increased total N retention by 1.9-2.3 g N/d (P < 0.01) over three successive 5 d periods. In contrast, wool growth was significantly reduced by 0.7 g N/d (P < 0.001) and the proportion of total N retained in wool declined from 0.71 to 0.25 (P < 0.01). The reduction in wool growth was accompanied by a decrease in protein fractional synthesis rate (PSR) in skin (11.6 v. 6.3 %/d, P < 0.01). Muscle protein FSR, on the other hand, was markedly stimulated during cimaterol infusion (1.45 v. 3.01%/d, P < 0.001) as was RNA concentration (P < 0.001), RNA:protein (P < 0.001) and protein:DNA (P < 0.05). The estimated increase in total protein synthesis in muscle (+24 to 30 g/d) due to cimaterol administration was counterbalanced by reductions for skin (-25 to 27 g/d); this may account for the lack of changes in whole-body protein synthesis following beta-agonist administration reported in other studies. Although N retention rapidly returned to control values following withdrawal of the drug, both wool growth and skin protein synthesis remained depressed, while muscle protein FSR declined, but not to pre-treatment values. These results suggest a persistent action of cimaterol, but whether this is a function of residue concentrations or long-term metabolic responses is not known.

Original languageEnglish
Pages (from-to)501-513
Number of pages13
JournalBritish Journal of Nutrition
Volume71
Issue number4
Publication statusPublished - Apr 1994

Keywords

  • beta-agonists
  • cimaterol
  • protein synthesis
  • wool growth growth
  • sheep
  • young growing sheep
  • skeletal-muscle
  • adrenergic agonist
  • body-composition
  • wether lambs
  • energy-metabolism
  • fed ractopamine
  • veal calves
  • amino-acids
  • food-intake

Cite this

NASH, J. E., ROCHA, H. J. G., Buchan, V., Calder, A. G., Milne, E., QUIRKE, J. F., & Lobley, G. (1994). The effect of acute and chronic administration of the beta-agonist, cimaterol, on protein synthesisin ovine skin and muscle. British Journal of Nutrition, 71(4), 501-513.

The effect of acute and chronic administration of the beta-agonist, cimaterol, on protein synthesisin ovine skin and muscle. / NASH, J E ; ROCHA, H J G ; Buchan, Vivien; Calder, Alexander Graham; Milne, Eric; QUIRKE, J F ; Lobley, Gerald.

In: British Journal of Nutrition, Vol. 71, No. 4, 04.1994, p. 501-513.

Research output: Contribution to journalArticle

NASH, J E ; ROCHA, H J G ; Buchan, Vivien ; Calder, Alexander Graham ; Milne, Eric ; QUIRKE, J F ; Lobley, Gerald. / The effect of acute and chronic administration of the beta-agonist, cimaterol, on protein synthesisin ovine skin and muscle. In: British Journal of Nutrition. 1994 ; Vol. 71, No. 4. pp. 501-513.
@article{4bbaa8249b024783bc06a6ed2831339e,
title = "The effect of acute and chronic administration of the beta-agonist, cimaterol, on protein synthesisin ovine skin and muscle",
abstract = "The action of intravenous infusion of the beta-agonist cimaterol (2.5 mg/d) on whole-body N retention and protein synthesis in peripheral tissues was examined in growing sheep. Wool growth was determined from skin patch clippings and adjusted to total fibre production. Protein synthesis was measured, using sequential large dose injections of [1-C-13]valine, leucine and phenylalanine and then [ring-d(5)]-phenylalanine, on biopsy samples from skin and m. longissimus dorsi taken before beta-agonist administration, at day 3 and day 15 of cimaterol infusion, and 15 d after withdrawal of the drug. Cimaterol increased total N retention by 1.9-2.3 g N/d (P < 0.01) over three successive 5 d periods. In contrast, wool growth was significantly reduced by 0.7 g N/d (P < 0.001) and the proportion of total N retained in wool declined from 0.71 to 0.25 (P < 0.01). The reduction in wool growth was accompanied by a decrease in protein fractional synthesis rate (PSR) in skin (11.6 v. 6.3 {\%}/d, P < 0.01). Muscle protein FSR, on the other hand, was markedly stimulated during cimaterol infusion (1.45 v. 3.01{\%}/d, P < 0.001) as was RNA concentration (P < 0.001), RNA:protein (P < 0.001) and protein:DNA (P < 0.05). The estimated increase in total protein synthesis in muscle (+24 to 30 g/d) due to cimaterol administration was counterbalanced by reductions for skin (-25 to 27 g/d); this may account for the lack of changes in whole-body protein synthesis following beta-agonist administration reported in other studies. Although N retention rapidly returned to control values following withdrawal of the drug, both wool growth and skin protein synthesis remained depressed, while muscle protein FSR declined, but not to pre-treatment values. These results suggest a persistent action of cimaterol, but whether this is a function of residue concentrations or long-term metabolic responses is not known.",
keywords = "beta-agonists, cimaterol, protein synthesis, wool growth growth, sheep, young growing sheep, skeletal-muscle, adrenergic agonist, body-composition, wether lambs, energy-metabolism, fed ractopamine, veal calves, amino-acids, food-intake",
author = "NASH, {J E} and ROCHA, {H J G} and Vivien Buchan and Calder, {Alexander Graham} and Eric Milne and QUIRKE, {J F} and Gerald Lobley",
year = "1994",
month = "4",
language = "English",
volume = "71",
pages = "501--513",
journal = "British Journal of Nutrition",
issn = "0007-1145",
publisher = "Cambridge Univ. Press.",
number = "4",

}

TY - JOUR

T1 - The effect of acute and chronic administration of the beta-agonist, cimaterol, on protein synthesisin ovine skin and muscle

AU - NASH, J E

AU - ROCHA, H J G

AU - Buchan, Vivien

AU - Calder, Alexander Graham

AU - Milne, Eric

AU - QUIRKE, J F

AU - Lobley, Gerald

PY - 1994/4

Y1 - 1994/4

N2 - The action of intravenous infusion of the beta-agonist cimaterol (2.5 mg/d) on whole-body N retention and protein synthesis in peripheral tissues was examined in growing sheep. Wool growth was determined from skin patch clippings and adjusted to total fibre production. Protein synthesis was measured, using sequential large dose injections of [1-C-13]valine, leucine and phenylalanine and then [ring-d(5)]-phenylalanine, on biopsy samples from skin and m. longissimus dorsi taken before beta-agonist administration, at day 3 and day 15 of cimaterol infusion, and 15 d after withdrawal of the drug. Cimaterol increased total N retention by 1.9-2.3 g N/d (P < 0.01) over three successive 5 d periods. In contrast, wool growth was significantly reduced by 0.7 g N/d (P < 0.001) and the proportion of total N retained in wool declined from 0.71 to 0.25 (P < 0.01). The reduction in wool growth was accompanied by a decrease in protein fractional synthesis rate (PSR) in skin (11.6 v. 6.3 %/d, P < 0.01). Muscle protein FSR, on the other hand, was markedly stimulated during cimaterol infusion (1.45 v. 3.01%/d, P < 0.001) as was RNA concentration (P < 0.001), RNA:protein (P < 0.001) and protein:DNA (P < 0.05). The estimated increase in total protein synthesis in muscle (+24 to 30 g/d) due to cimaterol administration was counterbalanced by reductions for skin (-25 to 27 g/d); this may account for the lack of changes in whole-body protein synthesis following beta-agonist administration reported in other studies. Although N retention rapidly returned to control values following withdrawal of the drug, both wool growth and skin protein synthesis remained depressed, while muscle protein FSR declined, but not to pre-treatment values. These results suggest a persistent action of cimaterol, but whether this is a function of residue concentrations or long-term metabolic responses is not known.

AB - The action of intravenous infusion of the beta-agonist cimaterol (2.5 mg/d) on whole-body N retention and protein synthesis in peripheral tissues was examined in growing sheep. Wool growth was determined from skin patch clippings and adjusted to total fibre production. Protein synthesis was measured, using sequential large dose injections of [1-C-13]valine, leucine and phenylalanine and then [ring-d(5)]-phenylalanine, on biopsy samples from skin and m. longissimus dorsi taken before beta-agonist administration, at day 3 and day 15 of cimaterol infusion, and 15 d after withdrawal of the drug. Cimaterol increased total N retention by 1.9-2.3 g N/d (P < 0.01) over three successive 5 d periods. In contrast, wool growth was significantly reduced by 0.7 g N/d (P < 0.001) and the proportion of total N retained in wool declined from 0.71 to 0.25 (P < 0.01). The reduction in wool growth was accompanied by a decrease in protein fractional synthesis rate (PSR) in skin (11.6 v. 6.3 %/d, P < 0.01). Muscle protein FSR, on the other hand, was markedly stimulated during cimaterol infusion (1.45 v. 3.01%/d, P < 0.001) as was RNA concentration (P < 0.001), RNA:protein (P < 0.001) and protein:DNA (P < 0.05). The estimated increase in total protein synthesis in muscle (+24 to 30 g/d) due to cimaterol administration was counterbalanced by reductions for skin (-25 to 27 g/d); this may account for the lack of changes in whole-body protein synthesis following beta-agonist administration reported in other studies. Although N retention rapidly returned to control values following withdrawal of the drug, both wool growth and skin protein synthesis remained depressed, while muscle protein FSR declined, but not to pre-treatment values. These results suggest a persistent action of cimaterol, but whether this is a function of residue concentrations or long-term metabolic responses is not known.

KW - beta-agonists

KW - cimaterol

KW - protein synthesis

KW - wool growth growth

KW - sheep

KW - young growing sheep

KW - skeletal-muscle

KW - adrenergic agonist

KW - body-composition

KW - wether lambs

KW - energy-metabolism

KW - fed ractopamine

KW - veal calves

KW - amino-acids

KW - food-intake

M3 - Article

VL - 71

SP - 501

EP - 513

JO - British Journal of Nutrition

JF - British Journal of Nutrition

SN - 0007-1145

IS - 4

ER -