Abstract
Short-term laboratory experiments were performed to investigate the effect of location on protozoan grazing of a genetically modified bacterial inoculum in soil. Pseudomonas fluorescens (strain 10586, containing chromosomally borne genes encoding bioluminescence and antibiotic resistances) was introduced into varying pore size classes by adjustment of the soil matric potential with reference to the moisture release characteristic. The soil ciliate protozoan Colpoda steinii was subsequently introduced to the soil at conditions close to field capacity to ensure initial location in larger pores.
When the Ps. fluorescens was predominantly located in small pores (less than 6 mum pore neck diameter), the decline in viable cell concentration was less than that when located in larger pores. This suggests that the bacterial inocula introduced into soil may be protected by spatial compartmentalisation. This protection may be from protozoan grazing, in which case the predator activity of the introduced Colpoda inoculum was not significant in comparison to that of the indigenous protozoa. Further work is therefore required to determine the mechanism of protection but the findings demonstrate that the antecedent matric potential and pore size characteristics will be critical in determining the survival characteristics of microbial inocula in soil.
| Original language | English |
|---|---|
| Pages (from-to) | 633-640 |
| Number of pages | 8 |
| Journal | Geoderma |
| Volume | 56 |
| Issue number | 1-4 |
| Publication status | Published - 15 Mar 1993 |
Keywords
- LEGUMINOSARUM BIOVAR TRIFOLII
- POPULATION-DYNAMICS
- PREDATORS
- SURVIVAL
- PREY
Cite this
THE EFFECT OF LOCATION IN SOIL ON PROTOZOAL GRAZING OF A GENETICALLY MODIFIED BACTERIAL INOCULUM. / Wright, Alison Jane; Killham, Kenneth Stuart; Glover, Lesley Anne; Prosser, James Ivor.
In: Geoderma, Vol. 56, No. 1-4, 15.03.1993, p. 633-640.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - THE EFFECT OF LOCATION IN SOIL ON PROTOZOAL GRAZING OF A GENETICALLY MODIFIED BACTERIAL INOCULUM
AU - Wright, Alison Jane
AU - Killham, Kenneth Stuart
AU - Glover, Lesley Anne
AU - Prosser, James Ivor
PY - 1993/3/15
Y1 - 1993/3/15
N2 - Short-term laboratory experiments were performed to investigate the effect of location on protozoan grazing of a genetically modified bacterial inoculum in soil. Pseudomonas fluorescens (strain 10586, containing chromosomally borne genes encoding bioluminescence and antibiotic resistances) was introduced into varying pore size classes by adjustment of the soil matric potential with reference to the moisture release characteristic. The soil ciliate protozoan Colpoda steinii was subsequently introduced to the soil at conditions close to field capacity to ensure initial location in larger pores.When the Ps. fluorescens was predominantly located in small pores (less than 6 mum pore neck diameter), the decline in viable cell concentration was less than that when located in larger pores. This suggests that the bacterial inocula introduced into soil may be protected by spatial compartmentalisation. This protection may be from protozoan grazing, in which case the predator activity of the introduced Colpoda inoculum was not significant in comparison to that of the indigenous protozoa. Further work is therefore required to determine the mechanism of protection but the findings demonstrate that the antecedent matric potential and pore size characteristics will be critical in determining the survival characteristics of microbial inocula in soil.
AB - Short-term laboratory experiments were performed to investigate the effect of location on protozoan grazing of a genetically modified bacterial inoculum in soil. Pseudomonas fluorescens (strain 10586, containing chromosomally borne genes encoding bioluminescence and antibiotic resistances) was introduced into varying pore size classes by adjustment of the soil matric potential with reference to the moisture release characteristic. The soil ciliate protozoan Colpoda steinii was subsequently introduced to the soil at conditions close to field capacity to ensure initial location in larger pores.When the Ps. fluorescens was predominantly located in small pores (less than 6 mum pore neck diameter), the decline in viable cell concentration was less than that when located in larger pores. This suggests that the bacterial inocula introduced into soil may be protected by spatial compartmentalisation. This protection may be from protozoan grazing, in which case the predator activity of the introduced Colpoda inoculum was not significant in comparison to that of the indigenous protozoa. Further work is therefore required to determine the mechanism of protection but the findings demonstrate that the antecedent matric potential and pore size characteristics will be critical in determining the survival characteristics of microbial inocula in soil.
KW - LEGUMINOSARUM BIOVAR TRIFOLII
KW - POPULATION-DYNAMICS
KW - PREDATORS
KW - SURVIVAL
KW - PREY
M3 - Article
VL - 56
SP - 633
EP - 640
JO - Geoderma
JF - Geoderma
SN - 0016-7061
IS - 1-4
ER -