The effects of graded levels of calorie restriction: III. Impact of short term calorie and protein restriction on mean daily body temperature and torpor use in the C57BL/6 mouse

Sharon E. Mitchell, Camille Delville, Penelope Konstantopedos, Davina Derous, Cara L. Green, Luonan Chen, Jing-Dong J. Han, Yingchun Wang, Daniel E.L. Promislow, Alex Douglas, David Lusseau, John R. Speakman

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A commonly observed response in mammals to calorie restriction (CR) is reduced body temperature (T b ). We explored how the T b of male C57BL/6 mice responded to graded CR (10 to 40%), compared to the response to equivalent levels of protein restriction (PR) over 3 months. Under CR there was a dynamic change in daily T b over the first 30–35 days, which stabilized thereafter until day 70 after which a further decline was noted. The time to reach stability was dependent on restriction level. Body mass negatively correlated with T b under ad libitum feeding and positively correlated under CR. The average T b over the last 20 days was significantly related to the levels of body fat, structural tissue, leptin and insulin-like growth factor-1. Some mice, particularly those under higher levels of CR, showed periods of daily torpor later in the restriction period. None of the changes in T b under CR were recapitulated by equivalent levels of PR. We conclude that changes in T b under CR are a response only to the shortfall in calorie intake. The linear relationship between average T b and the level of restriction supports the idea that T b changes are an integral aspect of the lifespan effect.
Original languageEnglish
Pages (from-to)18314-18337
Number of pages24
Issue number21
Publication statusPublished - 22 Jul 2015



  • Gerotarget
  • Body Temperature
  • calorie restriction
  • dietary restriction
  • protein restriction
  • Torpor

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