The human beta-glucan receptor is widely expressed and functionally equivalent to murine Dectin-1 on primary cells

Janet A Willment, Andrew S J Marshall, Delyth M Reid, David L Williams, Simon Y C Wong, Siamon Gordon, Gordon D Brown

Research output: Contribution to journalArticle

184 Citations (Scopus)

Abstract

We identified the C-type-lectin-like receptor, Dectin-1, as the major receptor for fungal beta-glucans on murine macrophages and have demonstrated that it plays a significant role in the cellular response to these carbohydrates. Using two novel, isoform-specific mAb, we show here that human Dectin-1, the beta-glucan receptor (betaGR), is widely expressed and present on all monocyte populations as well as macrophages, DC, neutrophils and eosinophils. This receptor is also expressed on B cells and a subpopulation of T cells, demonstrating that human Dectin-1 is not myeloid restricted. Both major functional betaGR isoforms - betaGR-A and betaGR-B - were expressed by these cell populations in peripheral blood; however, only betaGR-B was significantly expressed on mature monocyte-derived macrophages and immature DC, suggesting cell-specific control of isoform expression. Inflammatory cells, recruited in vivo using a new skin-window technique, demonstrated that Dectin-1 expression was not significantly modulated on macrophages during inflammation, but is decreased on recruited granulocytes. Despite previous reports detailing the involvement of other beta-glucan receptors on mature human macrophages, we have demonstrated that Dectin-1 acted as the major beta-glucan receptor on these cells and contributed to the inflammatory response to these carbohydrates.
Original languageEnglish
Pages (from-to)1539-1547
Number of pages9
JournalEuropean Journal of Immunology
Volume35
Issue number5
Early online date7 Apr 2005
DOIs
Publication statusPublished - May 2005

Keywords

  • Animals
  • Antibodies, Monoclonal
  • Dendritic Cells
  • Eosinophils
  • Flow Cytometry
  • Humans
  • Macrophages
  • Membrane Proteins
  • Mice
  • Nerve Tissue Proteins
  • Neutrophils
  • Receptors, Immunologic
  • Species Specificity

Cite this

Willment, J. A., Marshall, A. S. J., Reid, D. M., Williams, D. L., Wong, S. Y. C., Gordon, S., & Brown, G. D. (2005). The human beta-glucan receptor is widely expressed and functionally equivalent to murine Dectin-1 on primary cells. European Journal of Immunology, 35(5), 1539-1547. https://doi.org/10.1002/eji.200425725