The metal tolerance profile of Thlaspi goesingense is mimicked in Arabidopsis thaliana heterologously expressing serine acetyl-transferase

John L Freeman, David E Salt

Research output: Contribution to journalArticle

26 Citations (Scopus)
4 Downloads (Pure)

Abstract

Background: The Ni hyperaccumulator Thlaspi goesingense is tolerant to Ni congruent to Zn congruent to Co and slightly resistant to > Cd. We previously observed that elevated glutathione, driven by constitutive activation of serine acetyltransferase ( SAT), plays a role in the Ni tolerance of T. goesingense.

Results: Here we show that the elevated shoot concentration of glutathione, previously shown to cause elevated Ni tolerance in Arabidopsis thaliana heterologously expressing T. goesingense mitochondrial serine acetyltransferase ( SATm), also causes tolerance to Co and Zn while slightly enhancing resistance to Cd. The level of tolerance afforded to each metal is ranked Ni congruent to Co, > Zn > Cd. The Ni congruent to Co, > Zn tolerances are positively correlated with both the accumulation of glutathione ( GSH) and the ability to resist the oxidative damage induced by these different metals. Based on the relative concentrations of each metal used a relatively low level of resistance to Cd was observed in both T. goesingense and TgSATm expressing lines and Cd resistance was least correlated to GSH accumulation.

Conclusion: Such data supports the conclusion that elevated glutathione levels, driven by constitutively enhanced SAT activity in the hyperaccumulator T. goesingense, plays an important role in the Ni, Co and Zn tolerance of this and other Thlaspi species. The hyper-activation of S assimilation through SAT is an excellent strategy for engineering enhanced metal tolerance in transgenic plants potentially used for phytoremediation.

Original languageEnglish
Article number63
Number of pages10
JournalBMC Plant Biology
Volume7
DOIs
Publication statusPublished - 28 Nov 2007

Keywords

  • gamma-glutamylcysteine synthetase
  • oxidative stress
  • plant-responses
  • molecular mechanisms
  • active oxygen
  • gene family
  • glutathione
  • nickel
  • zinc
  • acetyltransferase

Cite this

@article{42a8139d0957486facec8436352f61fd,
title = "The metal tolerance profile of Thlaspi goesingense is mimicked in Arabidopsis thaliana heterologously expressing serine acetyl-transferase",
abstract = "Background: The Ni hyperaccumulator Thlaspi goesingense is tolerant to Ni congruent to Zn congruent to Co and slightly resistant to > Cd. We previously observed that elevated glutathione, driven by constitutive activation of serine acetyltransferase ( SAT), plays a role in the Ni tolerance of T. goesingense.Results: Here we show that the elevated shoot concentration of glutathione, previously shown to cause elevated Ni tolerance in Arabidopsis thaliana heterologously expressing T. goesingense mitochondrial serine acetyltransferase ( SATm), also causes tolerance to Co and Zn while slightly enhancing resistance to Cd. The level of tolerance afforded to each metal is ranked Ni congruent to Co, > Zn > Cd. The Ni congruent to Co, > Zn tolerances are positively correlated with both the accumulation of glutathione ( GSH) and the ability to resist the oxidative damage induced by these different metals. Based on the relative concentrations of each metal used a relatively low level of resistance to Cd was observed in both T. goesingense and TgSATm expressing lines and Cd resistance was least correlated to GSH accumulation.Conclusion: Such data supports the conclusion that elevated glutathione levels, driven by constitutively enhanced SAT activity in the hyperaccumulator T. goesingense, plays an important role in the Ni, Co and Zn tolerance of this and other Thlaspi species. The hyper-activation of S assimilation through SAT is an excellent strategy for engineering enhanced metal tolerance in transgenic plants potentially used for phytoremediation.",
keywords = "gamma-glutamylcysteine synthetase, oxidative stress, plant-responses, molecular mechanisms, active oxygen, gene family, glutathione, nickel, zinc, acetyltransferase",
author = "Freeman, {John L} and Salt, {David E}",
year = "2007",
month = "11",
day = "28",
doi = "10.1186/1471-2229-7-63",
language = "English",
volume = "7",
journal = "BMC Plant Biology",
issn = "1471-2229",
publisher = "BioMed Central",

}

TY - JOUR

T1 - The metal tolerance profile of Thlaspi goesingense is mimicked in Arabidopsis thaliana heterologously expressing serine acetyl-transferase

AU - Freeman, John L

AU - Salt, David E

PY - 2007/11/28

Y1 - 2007/11/28

N2 - Background: The Ni hyperaccumulator Thlaspi goesingense is tolerant to Ni congruent to Zn congruent to Co and slightly resistant to > Cd. We previously observed that elevated glutathione, driven by constitutive activation of serine acetyltransferase ( SAT), plays a role in the Ni tolerance of T. goesingense.Results: Here we show that the elevated shoot concentration of glutathione, previously shown to cause elevated Ni tolerance in Arabidopsis thaliana heterologously expressing T. goesingense mitochondrial serine acetyltransferase ( SATm), also causes tolerance to Co and Zn while slightly enhancing resistance to Cd. The level of tolerance afforded to each metal is ranked Ni congruent to Co, > Zn > Cd. The Ni congruent to Co, > Zn tolerances are positively correlated with both the accumulation of glutathione ( GSH) and the ability to resist the oxidative damage induced by these different metals. Based on the relative concentrations of each metal used a relatively low level of resistance to Cd was observed in both T. goesingense and TgSATm expressing lines and Cd resistance was least correlated to GSH accumulation.Conclusion: Such data supports the conclusion that elevated glutathione levels, driven by constitutively enhanced SAT activity in the hyperaccumulator T. goesingense, plays an important role in the Ni, Co and Zn tolerance of this and other Thlaspi species. The hyper-activation of S assimilation through SAT is an excellent strategy for engineering enhanced metal tolerance in transgenic plants potentially used for phytoremediation.

AB - Background: The Ni hyperaccumulator Thlaspi goesingense is tolerant to Ni congruent to Zn congruent to Co and slightly resistant to > Cd. We previously observed that elevated glutathione, driven by constitutive activation of serine acetyltransferase ( SAT), plays a role in the Ni tolerance of T. goesingense.Results: Here we show that the elevated shoot concentration of glutathione, previously shown to cause elevated Ni tolerance in Arabidopsis thaliana heterologously expressing T. goesingense mitochondrial serine acetyltransferase ( SATm), also causes tolerance to Co and Zn while slightly enhancing resistance to Cd. The level of tolerance afforded to each metal is ranked Ni congruent to Co, > Zn > Cd. The Ni congruent to Co, > Zn tolerances are positively correlated with both the accumulation of glutathione ( GSH) and the ability to resist the oxidative damage induced by these different metals. Based on the relative concentrations of each metal used a relatively low level of resistance to Cd was observed in both T. goesingense and TgSATm expressing lines and Cd resistance was least correlated to GSH accumulation.Conclusion: Such data supports the conclusion that elevated glutathione levels, driven by constitutively enhanced SAT activity in the hyperaccumulator T. goesingense, plays an important role in the Ni, Co and Zn tolerance of this and other Thlaspi species. The hyper-activation of S assimilation through SAT is an excellent strategy for engineering enhanced metal tolerance in transgenic plants potentially used for phytoremediation.

KW - gamma-glutamylcysteine synthetase

KW - oxidative stress

KW - plant-responses

KW - molecular mechanisms

KW - active oxygen

KW - gene family

KW - glutathione

KW - nickel

KW - zinc

KW - acetyltransferase

U2 - 10.1186/1471-2229-7-63

DO - 10.1186/1471-2229-7-63

M3 - Article

VL - 7

JO - BMC Plant Biology

JF - BMC Plant Biology

SN - 1471-2229

M1 - 63

ER -