The microbial community structure of different permeable sandy sediments characterized by the investigation of bacterial fatty acids and fluorescence in situ hybridization

SI Buhring*, M Elvert, U Witte

*Corresponding author for this work

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

This study describes the microbial community structure of three sandy sediment stations that differed with respect to median grain size and permeability in the German Bight of the Southern North Sea. The microbial community was investigated using lipid biomarker analyses and fluorescence in situ hybridization. For further characterization we determined the stable carbon isotope composition of the biomarkers. Biomarkers identified belong to different bacterial groups such as members of the Cytophaga-Flavobacterium cluster and sulfate-reducing bacteria (SRB). To support these findings, investigations using different fluorescent in situ hybridization probes were performed, specifically targeting Cytophaga-Flavobacterium, gamma-Proteobacteria and different members of the SRB. Depth profiles of bacterial fatty acid relative abundances revealed elevated subsurface peaks for the fine sediment, whereas at the other sandy sediment stations the concentrations were less variable with depth. Although oxygen penetrates deeper into the coarser and more permeable sediments, the SRB biomarkers are similarly abundant, indicating suboxic to anoxic niches in these environments. We detected SRB in all sediment types as well as in the surface and at greater depth, which suggests that SRB play a more important role in oxygenated marine sediments than previously thought.

Original languageEnglish
Pages (from-to)281-293
Number of pages13
JournalEnvironmental Microbiology
Volume7
Issue number2
DOIs
Publication statusPublished - Feb 2005

Keywords

  • SULFATE-REDUCING BACTERIA
  • ANAEROBIC METHANE OXIDATION
  • MARINE-SEDIMENTS
  • ADVECTIVE TRANSPORT
  • ORGANIC-MATTER
  • SEA SEDIMENTS
  • WADDEN SEA
  • VERTICAL-DISTRIBUTION
  • STABLE-ISOTOPES
  • CARBON ISOTOPES

Cite this

@article{4bf33c3695fb4b439831934e7c240cc7,
title = "The microbial community structure of different permeable sandy sediments characterized by the investigation of bacterial fatty acids and fluorescence in situ hybridization",
abstract = "This study describes the microbial community structure of three sandy sediment stations that differed with respect to median grain size and permeability in the German Bight of the Southern North Sea. The microbial community was investigated using lipid biomarker analyses and fluorescence in situ hybridization. For further characterization we determined the stable carbon isotope composition of the biomarkers. Biomarkers identified belong to different bacterial groups such as members of the Cytophaga-Flavobacterium cluster and sulfate-reducing bacteria (SRB). To support these findings, investigations using different fluorescent in situ hybridization probes were performed, specifically targeting Cytophaga-Flavobacterium, gamma-Proteobacteria and different members of the SRB. Depth profiles of bacterial fatty acid relative abundances revealed elevated subsurface peaks for the fine sediment, whereas at the other sandy sediment stations the concentrations were less variable with depth. Although oxygen penetrates deeper into the coarser and more permeable sediments, the SRB biomarkers are similarly abundant, indicating suboxic to anoxic niches in these environments. We detected SRB in all sediment types as well as in the surface and at greater depth, which suggests that SRB play a more important role in oxygenated marine sediments than previously thought.",
keywords = "SULFATE-REDUCING BACTERIA, ANAEROBIC METHANE OXIDATION, MARINE-SEDIMENTS, ADVECTIVE TRANSPORT, ORGANIC-MATTER, SEA SEDIMENTS, WADDEN SEA, VERTICAL-DISTRIBUTION, STABLE-ISOTOPES, CARBON ISOTOPES",
author = "SI Buhring and M Elvert and U Witte",
year = "2005",
month = "2",
doi = "10.1111/j.1462-2920.2004.00710.x",
language = "English",
volume = "7",
pages = "281--293",
journal = "Environmental Microbiology",
issn = "1462-2912",
publisher = "BLACKWELL PUBLISHING LTD",
number = "2",

}

TY - JOUR

T1 - The microbial community structure of different permeable sandy sediments characterized by the investigation of bacterial fatty acids and fluorescence in situ hybridization

AU - Buhring, SI

AU - Elvert, M

AU - Witte, U

PY - 2005/2

Y1 - 2005/2

N2 - This study describes the microbial community structure of three sandy sediment stations that differed with respect to median grain size and permeability in the German Bight of the Southern North Sea. The microbial community was investigated using lipid biomarker analyses and fluorescence in situ hybridization. For further characterization we determined the stable carbon isotope composition of the biomarkers. Biomarkers identified belong to different bacterial groups such as members of the Cytophaga-Flavobacterium cluster and sulfate-reducing bacteria (SRB). To support these findings, investigations using different fluorescent in situ hybridization probes were performed, specifically targeting Cytophaga-Flavobacterium, gamma-Proteobacteria and different members of the SRB. Depth profiles of bacterial fatty acid relative abundances revealed elevated subsurface peaks for the fine sediment, whereas at the other sandy sediment stations the concentrations were less variable with depth. Although oxygen penetrates deeper into the coarser and more permeable sediments, the SRB biomarkers are similarly abundant, indicating suboxic to anoxic niches in these environments. We detected SRB in all sediment types as well as in the surface and at greater depth, which suggests that SRB play a more important role in oxygenated marine sediments than previously thought.

AB - This study describes the microbial community structure of three sandy sediment stations that differed with respect to median grain size and permeability in the German Bight of the Southern North Sea. The microbial community was investigated using lipid biomarker analyses and fluorescence in situ hybridization. For further characterization we determined the stable carbon isotope composition of the biomarkers. Biomarkers identified belong to different bacterial groups such as members of the Cytophaga-Flavobacterium cluster and sulfate-reducing bacteria (SRB). To support these findings, investigations using different fluorescent in situ hybridization probes were performed, specifically targeting Cytophaga-Flavobacterium, gamma-Proteobacteria and different members of the SRB. Depth profiles of bacterial fatty acid relative abundances revealed elevated subsurface peaks for the fine sediment, whereas at the other sandy sediment stations the concentrations were less variable with depth. Although oxygen penetrates deeper into the coarser and more permeable sediments, the SRB biomarkers are similarly abundant, indicating suboxic to anoxic niches in these environments. We detected SRB in all sediment types as well as in the surface and at greater depth, which suggests that SRB play a more important role in oxygenated marine sediments than previously thought.

KW - SULFATE-REDUCING BACTERIA

KW - ANAEROBIC METHANE OXIDATION

KW - MARINE-SEDIMENTS

KW - ADVECTIVE TRANSPORT

KW - ORGANIC-MATTER

KW - SEA SEDIMENTS

KW - WADDEN SEA

KW - VERTICAL-DISTRIBUTION

KW - STABLE-ISOTOPES

KW - CARBON ISOTOPES

U2 - 10.1111/j.1462-2920.2004.00710.x

DO - 10.1111/j.1462-2920.2004.00710.x

M3 - Article

VL - 7

SP - 281

EP - 293

JO - Environmental Microbiology

JF - Environmental Microbiology

SN - 1462-2912

IS - 2

ER -